Enterobactin synthase component E (P10378)

Identification
Name:Enterobactin synthase component E
Synonyms:
  • Enterochelin synthase E
  • 2,3-dihydroxybenzoate-AMP ligase
  • Dihydroxybenzoic acid-activating enzyme
  • S-dihydroxybenzoyltransferase
Gene Name:entE
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-entB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with L-serine
Cellular Location:Not Available
SMPDB Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
  • Enterobactin Biosynthesis PW002048
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.02-Pyrocatechuic acid ↔ 1.0Pyrophosphate + 1.0(2,3-Dihydroxybenzoyl)adenylic acid
ReactionCard
SMPDB Reactions:
6.0Thumb+3.0L-Serine+3.0Thumb+3.0Thumb → 6.0Thumb+1.0enterobactin+6.0Pyrophosphate+3.0Thumb
6.0Adenosine triphosphate + 3.0L-Serine + 3.02,3-Dihydroxybenzoic acid + 3.0L-Serine → 6.0Adenosine monophosphate + 1.0enterobactin + 6.0Pyrophosphate + 3.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Adenosine triphosphate + 1.02,3-Dihydroxybenzoic acid → 1.0Pyrophosphate + 1.0(2,3-Dihydroxybenzoyl)adenylic acid
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02,3-Dihydroxybenzoic acid + 1.0Adenosine triphosphate + 1.0Hydrogen ion → 1.0(2,3-Dihydroxybenzoyl)adenylic acid + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0a holo-[EntB isochorismatase/aryl-carrier protein] → 1.0a 2,3-dihydroxybenzoyl-[EntB isochorismatase/aryl-carrier protein]+1.0Thumb+1.0Thumb
1.0(2,3-Dihydroxybenzoyl)adenylic acid + 1.0a holo-[EntB isochorismatase/aryl-carrier protein] → 1.0a 2,3-dihydroxybenzoyl-[EntB isochorismatase/aryl-carrier protein] + 1.0Adenosine monophosphate + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Pyrocatechuic acid + 1.0Adenosine triphosphate + 1.0Hydrogen ion → 1.0(2,3-Dihydroxybenzoyl)adenylic acid + 1.0Pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.02-Pyrocatechuic acid → 1.0Pyrophosphate + 1.0(2,3-Dihydroxybenzoyl)adenylic acid
ReactionCard
1.0Thumb+1.0holo-entB → 1.0Thumb+1.0acyl-holo-entB
1.0(2,3-Dihydroxybenzoyl)adenylic acid + 1.0holo-entB → 1.0Adenosine monophosphate + 1.0acyl-holo-entB
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04053(2,3-Dihydroxybenzoyl)adenylic acidMetaboCard
ECMDB241842,3-Dihydroxybenzoic acidMetaboCard
ECMDB003972-Pyrocatechuic acidMetaboCard
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00187L-SerineMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
(2,3-dihydroxybenzoyl)adenylate synthase activity
adenylyltransferase activity
catalytic activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular metabolic process
cofactor metabolic process
metabolic process
siderophore biosynthetic process
siderophore metabolic process
Gene Properties
Blattner:b0594
Gene OrientationClockwise
Centisome Percentage:13.48
Left Sequence End625293
Right Sequence End626903
Gene Sequence:
>1611 bp
ATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAG
GGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTAC
CACTCCCGTGACCTGAAAAACTGGTCGCTGGTCAGCACCCCGTTGGACCGCGTGTCGATG
CTGGACATGAAGGGCAACCCGGACTCCGGCGGCATCTGGGCGCCGTGCCTGAGCTACGCC
GACGGTAAATTCTGGCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAAC
GGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCG
ATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTAT
ATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAG
GCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACG
CCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATG
GCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATC
GACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAG
AACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTAC
ATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGC
GGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGAC
GGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTA
GCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTT
GACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCG
CGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCG
ACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCG
CCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGC
TACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGAC
CACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAG
AGCGTCTGGCTGCGGGTGGACGTGGATACGCTGGTCTACCGCTACAGCTACTCGTTTGAT
GGCGAGACGTGGCACACCGTGCCGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTAC
ATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGC
GGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:536
Protein Molecular Weight:59112
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Enterobactin synthase component E
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
PALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGEHNLQDAINHPAEDFTATPSP
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALIEGESRAQLA
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
References
External Links:
ResourceLink
Uniprot ID:P10378
Uniprot Name:ENTE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674415
Ecogene ID:EG10263
Ecocyc:EG10263
ColiBase:b0594
Kegg Gene:b0594
EchoBASE ID:EB0259
CCDB:ENTE_ECOLI
BacMap:16128577
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gehring, A. M., Bradley, K. A., Walsh, C. T. (1997). "Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate." Biochemistry 36:8495-8503. Pubmed: 9214294
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Liu, J., Duncan, K., Walsh, C. T. (1989). "Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase." J Bacteriol 171:791-798. Pubmed: 2521622
  • Saravani, G. A., Martin, D. R. (1990). "Opacity factor from group A streptococci is an apoproteinase." FEMS Microbiol Lett 56:35-39. Pubmed: 2110093
  • Staab, J. F., Elkins, M. F., Earhart, C. F. (1989). "Nucleotide sequence of the Escherichia coli entE gene." FEMS Microbiol Lett 50:15-19. Pubmed: 2525505