Identification
Name:3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase
Synonyms:
  • 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
  • 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate dehydrogenase
  • CI-dihydrodiol dehydrogenase
  • Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
  • Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase
  • PP-dihydrodiol dehydrogenase
Gene Name:hcaB
Enzyme Class:
Biological Properties
General Function:Not Available
Specific Function:Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively (By similarity). Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3-dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively (By similarity).
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Microbial metabolism in diverse environments ec01120
  • Phenylalanine metabolism ec00360
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.02-Hydroxy-3-(4-hydroxyphenyl)propenoic acid+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB249152-Hydroxy-3-(4-hydroxyphenyl)propenoic acidMetaboCard
ECMDB200643-(2,3-Dihydroxyphenyl)propanoateMetaboCard
ECMDB040613-(2,3-Dihydroxyphenyl)propionic acidMetaboCard
ECMDB20129cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diolMetaboCard
ECMDB21320Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diolMetaboCard
ECMDB20130cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diolMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB20197Trans-2,3-DihydroxycinnamateMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b2541
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>ENA|AAC75594|AAC75594.1 Escherichia coli str. K-12 substr. MG1655 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
ATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTG
GCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCG
GCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATATTCTGGCGGTGGAAGGTAAC
GTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTGACTCGTTCCGGCAAG
CTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACT
CCCGCAGAGACGCTCGAAACCGGCTTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTAC
CTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTC
ACACTGTCAAATGCCGCCTGGTATCCTGGCGGCGGTGGCCCGCTGTACACCGCCAGTAAA
CATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTG
AATGGCGTCGGCCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAA
AGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCCGCCATTTTACCGCTG
CAATTTTTCCCGCAACCGGCGGATTTTACGGGGCCGTATGTGATGTTGACATCGCGGCGC
AATAATCGCGCATTAAGCGGTGTGATGATCAACGCTGATGCGGGTTTAGCGATTCGCGGC
ATTCGCCACGTAGCGGCTGGGCTGGATCTTTAA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:Not Available
Protein Molecular Weight:Not Available
Protein Theoretical pI:Not Available
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>sp|P0CI31|HCAB_ECOLI 3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase OS=Escherichia coli (strain K12) GN=hcaB PE=3 SV=1
MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGN
VTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGY
LLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASKHAATGLIRQLAYELAPKVRV
NGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLTSRR
NNRALSGVMINADAGLAIRGIRHVAAGLDL
References
External Links:
ResourceLink
Uniprot ID:P0CI31
ColiBase:b2541
Kegg Gene:b2541
EchoBASE ID:EB3232
BacMap:16130466
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Diaz, E., Ferrandez, A., Garcia, J. L. (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." J Bacteriol 180:2915-2923. Pubmed: 9603882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837