Autoinducer 2 import system permease protein lsrD (P0AFS1)

Identification
Name:Autoinducer 2 import system permease protein lsrD
Synonyms:
  • AI-2 import system permease protein lsrD
Gene Name:lsrD
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Part of the ABC transporter complex lsrABCD involved in autoinducer 2 (AI-2) import. Probably responsible for the translocation of the substrate across the membrane (Probable)
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
SMPDB Reactions:
1.0(2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran+1.0Phosphoribosyl-ATP+1.0Thumb+1.0Thumb+1.0Thumb → 1.0(2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran+1.0Adenosine diphosphate+1.0Thumb+1.0Pyrophosphate+1.0Thumb
1.0(2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran + 1.0Phosphoribosyl-ATP + 1.0Water + 1.0(2R,4S)-2-Methyl-2,3,3,4-tetrahydroxytetrahydrofuran + 1.0Phosphoribosyl-ATP → 1.0(2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran + 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0Pyrophosphate + 1.0ADP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21368(2R,4S)-2-Methyl-2,3,3,4-tetrahydroxytetrahydrofuranMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04138Phosphoribosyl-ATPMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b1515
Gene OrientationClockwise
Centisome Percentage:34.53
Left Sequence End1602071
Right Sequence End1603063
Gene Sequence:
>993 bp
ATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACG
ATAGGGATGGGATTTGCCTGGCGCTATGCCAGCCAGGTTTGGCAGGTCAGCCACTGGTTA
GGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTATT
GCCCGACTCATACGCTTTCCGCATAGCGTGCTGGCGGAAGTTCGCCATCCAGTGCTGAGC
AGTTTTGTGAGTTTGTTTCCGGCAACGACGATGCTGGTGGCGATTGGTTTTGTTCCGTGG
TTTCGCCCACTGGCGGTGTGCCTGTTCAGTTTTGGTGTCGTGGTTCAGTTGGCTTATGCC
GCCTGGCAAACTGCGGGATTATGGCGCGGATCTCACCCTGAAGAAGCTACCACGCCTGGA
CTGTATCTGCCGACAGTTGCCAACAACTTTATCAGCGCAATGGCCTGTGGTGCGTTGGGC
TACACCGACGCCGGTCTGGTGTTTTTAGGCGCAGGCGTTTTCTCATGGCTAAGCCTTGAA
CCGGTGATCTTGCAGCGTCTGCGCAGTTCGGGAGAATTACCCACGGCACTGCGGACATCA
CTCGGCATTCAGCTCGCTCCTGCGCTGGTGGCTTGTAGTGCCTGGCTGAGCGTCAACGGC
GGCGAGGGTGACACGCTGGCGAAAATGCTTTTCGGTTATGGACTGCTGCAACTGCTGTTT
ATGCTACGTCTGATGCCATGGTATCTCTCCCAGCCATTTAATGCTTCATTCTGGAGTTTC
TCGTTCGGCGTATCTGCACTGGCAACCACCGGTTTGCATCTGGGGAGTGGCAGCGATAAT
GGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATA
CTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGC
GCCGTTTTAATGAAAGCAGAGGACAAAGAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:330
Protein Molecular Weight:34456
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 5-25
  • 43-63
  • 68-88
  • 89-109
  • 116-136
  • 160-180
  • 210-230
  • 238-258
  • 259-279
  • 286-306
Protein Sequence:
>Autoinducer 2 import system permease protein lsrD
MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFICIGIVALPLTMVIVSGG
IDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGLINAGLIIYTKVNPLVITL
GTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDVLGLPVPLIIFLICLLVFWLW
LHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMTGLASAVAAVLLVSYFGSARSDL
GASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLVGYLQQGLQMAGVPNQVSSALSGAL
LIVVVVGRSVSLHRQQIKEWLARRANNPLP
References
External Links:
ResourceLink
Uniprot ID:P0AFS1
Uniprot Name:LSRD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742336
Ecogene ID:EG13808
Ecocyc:EG13808
ColiBase:b1515
Kegg Gene:b1515
CCDB:LSRD_ECOLI
BacMap:16129474
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Wang, L., Hashimoto, Y., Tsao, C. Y., Valdes, J. J., Bentley, W. E. (2005). "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." J Bacteriol 187:2066-2076. Pubmed: 15743955
  • Xavier, K. B., Bassler, B. L. (2005). "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." J Bacteriol 187:238-248. Pubmed: 15601708