Glycine betaine-binding periplasmic protein (P0AFM2)

Identification
Name:Glycine betaine-binding periplasmic protein
Synonyms:Not Available
Gene Name:proX
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Member of a multicomponent binding-protein-dependent transport system (the proU transporter) which serves as the glycine betaine/L-proline transporter
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:Not Available
SMPDB Reactions:
1.0L-Proline+1.0Thumb+1.0Thumb+1.0Thumb → 1.0L-Proline+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Proline + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Proline → 1.0L-Proline + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0L-Proline → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0L-Proline
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Carnitine → 1.0ADP + 1.0Carnitine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Crotonobetaine → 1.0ADP + 1.0Crotonobetaine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00062CarnitineMetaboCard
ECMDB21321CrotonobetaineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00162L-ProlineMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b2679
Gene OrientationClockwise
Centisome Percentage:60.46
Left Sequence End2805154
Right Sequence End2806146
Gene Sequence:
>993 bp
ATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACG
ATAGGGATGGGATTTGCCTGGCGCTATGCCAGCCAGGTTTGGCAGGTCAGCCACTGGTTA
GGGGATGGGCTGGTGATTCTGGCGATGATCATCTGGGGATTATTGACTAGCGCATTTATT
GCCCGACTCATACGCTTTCCGCATAGCGTGCTGGCGGAAGTTCGCCATCCAGTGCTGAGC
AGTTTTGTGAGTTTGTTTCCGGCAACGACGATGCTGGTGGCGATTGGTTTTGTTCCGTGG
TTTCGCCCACTGGCGGTGTGCCTGTTCAGTTTTGGTGTCGTGGTTCAGTTGGCTTATGCC
GCCTGGCAAACTGCGGGATTATGGCGCGGATCTCACCCTGAAGAAGCTACCACGCCTGGA
CTGTATCTGCCGACAGTTGCCAACAACTTTATCAGCGCAATGGCCTGTGGTGCGTTGGGC
TACACCGACGCCGGTCTGGTGTTTTTAGGCGCAGGCGTTTTCTCATGGCTAAGCCTTGAA
CCGGTGATCTTGCAGCGTCTGCGCAGTTCGGGAGAATTACCCACGGCACTGCGGACATCA
CTCGGCATTCAGCTCGCTCCTGCGCTGGTGGCTTGTAGTGCCTGGCTGAGCGTCAACGGC
GGCGAGGGTGACACGCTGGCGAAAATGCTTTTCGGTTATGGACTGCTGCAACTGCTGTTT
ATGCTACGTCTGATGCCATGGTATCTCTCCCAGCCATTTAATGCTTCATTCTGGAGTTTC
TCGTTCGGCGTATCTGCACTGGCAACCACCGGTTTGCATCTGGGGAGTGGCAGCGATAAT
GGATTTTTCCATACGCTGGCGGTGCCGCTGTTTATCTTTACCAATTTTATTATTGCAATA
CTGCTCATCCGTACTTTTGCGCTTCTGATGCAGGGAAAATTGTTAGTCAGAACCGAGCGC
GCCGTTTTAATGAAAGCAGAGGACAAAGAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:330
Protein Molecular Weight:36022
Protein Theoretical pI:6
PDB File:1R9Q
Signaling Regions:
  • 1-21
Transmembrane Regions:
  • None
Protein Sequence:
>Glycine betaine-binding periplasmic protein
MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQTLLVSRALEKLGYTVN
KPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKKFYREGVFVNGAAQGYLID
KKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPGWGCEGAINHQLAAYELTNTV
THNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNELKPGKDVVWLQVPFSALPGDKNA
DTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLFAIMQLPVADINAQNAIMHDGKASE
GDIQGHVDGWIKAHQQQFDGWVNEALAAQK
References
External Links:
ResourceLink
Uniprot ID:P0AFM2
Uniprot Name:PROX_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742336
PDB ID:1R9Q
Ecogene ID:EG10773
Ecocyc:EG10773
ColiBase:b2679
Kegg Gene:b2679
EchoBASE ID:EB0766
CCDB:PROX_ECOLI
BacMap:16130593
General Reference:
  • Barron, A., Jung, J. U., Villarejo, M. (1987). "Purification and characterization of a glycine betaine binding protein from Escherichia coli." J Biol Chem 262:11841-11846. Pubmed: 3305496
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gowrishankar, J. (1989). "Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli." J Bacteriol 171:1923-1931. Pubmed: 2649479
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Schiefner, A., Breed, J., Bosser, L., Kneip, S., Gade, J., Holtmann, G., Diederichs, K., Welte, W., Bremer, E. (2004). "Cation-pi interactions as determinants for binding of the compatible solutes glycine betaine and proline betaine by the periplasmic ligand-binding protein ProX from Escherichia coli." J Biol Chem 279:5588-5596. Pubmed: 14612446
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837