Putrescine transport system permease protein potI (P0AFL1)

Identification
Name:Putrescine transport system permease protein potI
Synonyms:Not Available
Gene Name:potI
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Required for the activity of the bacterial periplasmic transport system of putrescine
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Putrescine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Putrescine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01414PutrescineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b0857
Gene OrientationClockwise
Centisome Percentage:19.32
Left Sequence End896307
Right Sequence End897152
Gene Sequence:
>846 bp
ATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTT
ACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGCTG
GTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCG
ATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCG
ATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT
GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCG
CTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATG
CTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCT
TCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACG
CCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGC
TGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGG
CCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCG
GAAATCAACGCCCTGGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCC
TGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGT
GGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:281
Protein Molecular Weight:30540
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 14-33
  • 69-88
  • 116-135
  • 141-160
  • 187-206
  • 244-263
Protein Sequence:
>Putrescine transport system permease protein potI
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDA
MMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLS
LLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGAT
PLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARRG
References
External Links:
ResourceLink
Uniprot ID:P0AFL1
Uniprot Name:POTI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651390
Ecogene ID:EG11632
Ecocyc:EG11632
ColiBase:b0857
Kegg Gene:b0857
EchoBASE ID:EB1589
CCDB:POTI_ECOLI
BacMap:16128825
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pistocchi, R., Kashiwagi, K., Miyamoto, S., Nukui, E., Sadakata, Y., Kobayashi, H., Igarashi, K. (1993). "Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome." J Biol Chem 268:146-152. Pubmed: 8416922