5,10-methylenetetrahydrofolate reductase (P0AEZ1)

Identification
Name:5,10-methylenetetrahydrofolate reductase
Synonyms:Not Available
Gene Name:metF
Enzyme Class:
Biological Properties
General Function:Involved in methylenetetrahydrofolate reductase (NADPH) activity
Specific Function:5-methyltetrahydrofolate + NAD(P)(+) = 5,10- methylenetetrahydrofolate + NAD(P)H
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
2.0Hydrogen ion + 1.05,10-Methylene-THF + 1.0NADH → 1.05-Methyltetrahydrofolic acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.05-Methyltetrahydrofolic acid + 1.0NADP ↔ 1.05,10-Methylene-THF + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.05-Methyltetrahydrofolic acid + 1.0NAD ↔ 1.05,10-Methylene-THF + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.05-Methyltetrahydrofolic acid + 1.0NAD + 1.0NADP ↔ 1.05,10-Methylene-THF + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.05,10-Methylene-THF+1.0NADPH+1.0Thumb+1.0Thumb+1.0Thumb → 1.05-Methyltetrahydrofolic acid+1.0Thumb+1.0Thumb
1.05,10-Methylene-THF + 1.0NADPH + 1.0Hydrogen ion + 1.05,10-Methylene-THF + 1.0NADPH → 1.05-Methyltetrahydrofolic acid + 1.0NADP + 1.05-Methyltetrahydrofolic acid
ReactionCard
EcoCyc Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
2.0Hydrogen ion + 1.05,10-Methylene-THF + 1.0NADH → 1.05-Methyltetrahydrofolic acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.05-Methyltetrahydrofolic acid + 1.0NAD ↔ 1.05,10-Methylene-THF + 1.0NADH + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0NAD(P)(+) → 1.0Thumb+1.0NAD(P)H
1.05-Methyltetrahydrofolic acid + 1.0NAD(P)(+) → 1.05,10-Methylene-THF + 1.0NAD(P)H
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB013545,10-Methylene-THFMetaboCard
ECMDB013965-Methyltetrahydrofolic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
catalytic activity
methylenetetrahydrofolate reductase (NADPH) activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-NH group of donors
oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
methionine biosynthetic process
methionine metabolic process
oxidation reduction
sulfur amino acid biosynthetic process
sulfur amino acid metabolic process
Gene Properties
Blattner:b3941
Gene OrientationClockwise
Centisome Percentage:89.03
Left Sequence End4130639
Right Sequence End4131529
Gene Sequence:
>891 bp
ATGAGCGGCTTACCTCTTATTTCGCGCCGTCGACTGTTAACGGCGATGGCGCTTTCTCCG
TTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCG
CTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCG
GATACCATCAACTATCGCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGAC
GTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATG
GTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTCGTATTGCGCCGGGTCGC
GGATTTAACTTCAGTGACGGCAAACAGCCGTTGGCGATGGCGCGTAAATCGCTGACGGAA
ATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGAC
TTTATCCGCAGCATGAAACCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACG
ACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCAGGAAATT
CTTGATGAGTACGGCATCCCAAATGCCTGGCAAGGGGAAACCAACTTCTGGGGCAGTACC
GCCGTCAGTATCGATCGTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCAC
GACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTT
GTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCG
GCAATGCACTTTGTGCGCGTTCTGGATAACGCCATCGGAGGTAAAGCGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:296
Protein Molecular Weight:33102
Protein Theoretical pI:6
PDB File:1B5T
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>5,10-methylenetetrahydrofolate reductase
MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTY
GANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGD
LPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRA
ITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMTNVRIPAWMAQMFD
GLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRPGL
References
External Links:
ResourceLink
Uniprot ID:P0AEZ1
Uniprot Name:METF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674358
PDB ID:1B5T
Ecogene ID:EG10585
Ecocyc:EG10585
ColiBase:b3941
Kegg Gene:b3941
EchoBASE ID:EB0580
CCDB:METF_ECOLI
BacMap:16131779
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Guenther, B. D., Sheppard, C. A., Tran, P., Rozen, R., Matthews, R. G., Ludwig, M. L. (1999). "The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia." Nat Struct Biol 6:359-365. Pubmed: 10201405
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
  • Saint-Girons, I., Duchange, N., Zakin, M. M., Park, I., Margarita, D., Ferrara, P., Cohen, G. N. (1983). "Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region." Nucleic Acids Res 11:6723-6732. Pubmed: 6356036