7-alpha-hydroxysteroid dehydrogenase (P0AET8)

Identification
Name:7-alpha-hydroxysteroid dehydrogenase
Synonyms:
  • 7-alpha-HSDH
Gene Name:hdhA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:7-alpha-dehydroxylation of cholic acid, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Cholic acid + 1.0NAD ↔ 1.0NADH + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB231673-Alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanateMetaboCard
ECMDB21424Cholic acidMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
GO Classification:
Function
binding
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b1619
Gene OrientationCounterclockwise
Centisome Percentage:36.54
Left Sequence End1695297
Right Sequence End1696064
Gene Sequence:
>768 bp
ATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACC
GGGCTTGTACAGCGTGGTGGTGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTT
TACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAACCCAGCTTTAATTCCCCCATC
GCCAAACTTGTCCAGGACTACCCGATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAG
GCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGG
CGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGC
AACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAA
TTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCC
TCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGC
TATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGC
GTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAAT
GATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATT
ATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:255
Protein Molecular Weight:26778
Protein Theoretical pI:5
PDB File:1FMC
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>7-alpha-hydroxysteroid dehydrogenase
MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVDEIQQLGG
QAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADFRRAYELNVF
SFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEK
NIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVS
GQILTVSGGGVQELN
References
External Links:
ResourceLink
Uniprot ID:P0AET8
Uniprot Name:HDHA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902959
PDB ID:1FMC
Ecogene ID:EG10425
Ecocyc:EG10425
ColiBase:b1619
Kegg Gene:b1619
EchoBASE ID:EB0420
CCDB:HDHA_ECOLI
BacMap:16129577
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Tanabe, T., Tanaka, N., Uchikawa, K., Kabashima, T., Ito, K., Nonaka, T., Mitsui, Y., Tsuru, M., Yoshimoto, T. (1998). "Roles of the Ser146, Tyr159, and Lys163 residues in the catalytic action of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli." J Biochem 124:634-641. Pubmed: 9722677
  • Tanaka, N., Nonaka, T., Tanabe, T., Yoshimoto, T., Tsuru, D., Mitsui, Y. (1996). "Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli." Biochemistry 35:7715-7730. Pubmed: 8672472
  • Yoshimoto, T., Higashi, H., Kanatani, A., Lin, X. S., Nagai, H., Oyama, H., Kurazono, K., Tsuru, D. (1991). "Cloning and sequencing of the 7 alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme." J Bacteriol 173:2173-2179. Pubmed: 2007545