Identification
Name:Glyoxylate carboligase
Synonyms:
  • Tartronate-semialdehyde synthase
Gene Name:gcl
Enzyme Class:
Biological Properties
General Function:Involved in tartronate-semialdehyde synthase activity
Specific Function:Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde)
Cellular Location:Not Available
SMPDB Pathways:
  • glycolate and glyoxylate degradation PW000827
  • glycolate and glyoxylate degradation II PW002021
KEGG Pathways:
KEGG Reactions:
2.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
2.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
2.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
EcoCyc Reactions:
2.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
2.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00119Glyoxylic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB06938Tartronate semialdehydeMetaboCard
GO Classification:
Function
binding
carbon-carbon lyase activity
carboxy-lyase activity
catalytic activity
cation binding
ion binding
lyase activity
magnesium ion binding
metal ion binding
tartronate-semialdehyde synthase activity
thiamin pyrophosphate binding
vitamin binding
Process
aldehyde catabolic process
cellular aldehyde metabolic process
cellular metabolic process
glyoxylate catabolic process
metabolic process
Gene Properties
Blattner:b0507
Gene OrientationClockwise
Centisome Percentage:11.49
Left Sequence End533140
Right Sequence End534921
Gene Sequence:
>1782 bp
ATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCG
TGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTC
AGTGGGCCGCTGCTTATCAGCTATTTTATCGACAATATGGTAGCGAAAAATAACCTGCCG
TTGAAAGTGGTTGCAGGGCTGGCTGCGGCGTATGTTGGGCTGCAACTGTTTGCCGCCGGG
CTACATTACGCGCAGTCGCTGCTGTTTAATCGGGCGGCAGTAGGCGTAGTGCAACAGTTG
CGTACCGACGTGATGGATGCTGCGTTACGCCAGCCATTAAGCGAGTTTGATACCCAACCC
GTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTT
ACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATG
TTCAGCCTCGACTGGCGAATGGCACTGGTGGCGATAATGATTTTCCCGGTGGTGCTGGTG
GTAATGGTGATATACCAGCGTTACAGCACGCCGATTGTCCGTCGTGTGCGCGCCTATTTG
GCGGATATCAACGACGGCTTTAACGAAATCATCAATGGCATGAGCGTTATCCAGCAGTTT
CGTCAGCAGGCGCGATTTGGCGAACGTATGGGGGAGGCCAGTCGTTCACACTATATGGCG
AGGATGCAAACCCTGCGCCTCGACGGTTTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCA
TCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAA
GTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAA
CTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAA
CTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACC
ATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAACATT
AATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAA
AGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTT
GATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTG
CAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGAT
ATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGT
AGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGG
CAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTT
GATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCG
GCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGAT
GCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAA
CTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCGAAGAG
CTGGCAGCCAGCGTGCGTGAAGAGGAATCATTGAGCGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:593
Protein Molecular Weight:64731
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glyoxylate carboligase
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
YTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
LPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMG
WGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVH
IDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLL
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
LGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
LMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE
References
External Links:
ResourceLink
Uniprot ID:P0AEP7
Uniprot Name:GCL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674589
Ecogene ID:EG11583
Ecocyc:EG11583
ColiBase:b0507
Kegg Gene:b0507
EchoBASE ID:EB1542
CCDB:GCL_ECOLI
BacMap:16128491
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chang, Y. Y., Wang, A. Y., Cronan, J. E. Jr (1993). "Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family." J Biol Chem 268:3911-3919. Pubmed: 8440684
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553