Bifunctional adenosylcobalamin biosynthesis protein cobU (P0AE76)

Identification
Name:Bifunctional adenosylcobalamin biosynthesis protein cobU
Synonyms:
  • Adenosylcobinamide kinase
  • Adenosylcobinamide-phosphate guanylyltransferase
Gene Name:cobU
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:ATP-dependent phosphorylation of adenosylcobinamide and adds GMP to adenosylcobinamide phosphate
Cellular Location:Not Available
SMPDB Pathways:
  • adenosylcobalamin salvage from cobinamide PW001884
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosyl cobinamide + 1.0Adenosine triphosphate → 1.0Adenosyl cobinamide phosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosyl cobinamide phosphate + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0Adenosylcobinamide-GDP + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosyl cobinamide + 1.0Adenosine triphosphate ↔ 1.0Adenosyl cobinamide phosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosyl cobinamide phosphate + 1.0Guanosine triphosphate ↔ 1.0Adenosylcobinamide-GDP + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosyl cobinamide + 1.0Guanosine triphosphate ↔ 1.0Adenosyl cobinamide phosphate + 1.0Guanosine diphosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Guanosine triphosphate + 1.0Adenosyl cobinamide ↔ 1.0Adenosyl cobinamide phosphate + 1.0ADP + 1.0Guanosine diphosphate
ReactionCard
SMPDB Reactions:
1.0Adenosyl cobinamide+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0adenosylcobinamide phosphate+1.0Thumb
1.0Adenosyl cobinamide + 1.0Adenosine triphosphate + 1.0Adenosyl cobinamide → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0adenosylcobinamide phosphate + 1.0ADP
ReactionCard
1.0adenosylcobinamide phosphate+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Adenosylcobinamide-GDP+1.0Thumb
1.0adenosylcobinamide phosphate + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0Pyrophosphate + 1.0Adenosylcobinamide-GDP + 1.0Adenosylcobinamide-GDP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosyl cobinamide + 1.0Adenosine triphosphate → 1.0Adenosyl cobinamide phosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosyl cobinamide phosphate + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0Adenosylcobinamide-GDP + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB06903Adenosyl cobinamideMetaboCard
ECMDB12184Adenosyl cobinamide phosphateMetaboCard
ECMDB12185Adenosylcobinamide-GDPMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
adenosylcobinamide kinase activity
binding
catalytic activity
guanylyltransferase activity
kinase activity
nucleotide binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular biosynthetic process
cofactor biosynthetic process
metabolic process
Gene Properties
Blattner:b1993
Gene OrientationCounterclockwise
Centisome Percentage:44.47
Left Sequence End2063243
Right Sequence End2063788
Gene Sequence:
>546 bp
ATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAAC
CAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATG
ATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGT
TTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGGCATTGATTTG
TGGATTGAGCTGGGGCTGCCGGATGAGCGGCGGATTAAGAAAGCCTGCACCCAGGCCGCA
GAAGTGGCGCTGTTTACCTATAATAGTCGGGCGGCGCAAATCTGGTGGCAGCAAAATCAG
AGCAAATGTGTGCAGTTTGCCAATCTTTCCGTCTGGTATCTGGACGATGAACAACTGGCG
AAAGTAAGCGCCTTTGCCGATCGTACCATGACGCTGCAGGCAACGATTCAGGATGGCGTG
ATCTGGTTATCGGATGATAAGAATAATCTGGAAGTGAACTTAACCGCCTGGCAACAACCT
TCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:181
Protein Molecular Weight:20164
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Bifunctional adenosylcobalamin biosynthesis protein cobU
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVER
WQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLI
AACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKI
K
References
External Links:
ResourceLink
Uniprot ID:P0AE76
Uniprot Name:COBU_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674376
Ecogene ID:EG13238
Ecocyc:EG13238
ColiBase:b1993
Kegg Gene:b1993
EchoBASE ID:EB3027
CCDB:COBU_ECOLI
BacMap:16129934
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Lawrence, J. G., Roth, J. R. (1995). "The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli." J Bacteriol 177:6371-6380. Pubmed: 7592411