Identification |
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Name: | Hydrogenase-2 large chain |
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Synonyms: | - HYD2
- Membrane-bound hydrogenase 2 large subunit
- NiFe hydrogenase
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Gene Name: | hybC |
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Enzyme Class: | |
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Biological Properties |
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General Function: | Involved in nickel cation binding |
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Specific Function: | This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake |
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Cellular Location: | Cell membrane; Peripheral membrane protein |
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SMPDB Pathways: | Not Available |
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KEGG Pathways: | - Microbial metabolism in diverse environments ec01120
- Trinitrotoluene degradation ec00633
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KEGG Reactions: | |
1.02,4-Diamino-6-nitrotoluene | ↔ | 1.02,4-Diamino-6-hydroxylaminotoluene |
| 1.02,4-Diamino-6-nitrotoluene ↔ 1.02,4-Diamino-6-hydroxylaminotoluene ReactionCard | |
1.0 | + | 1.0Acceptor | ↔ | 1.0Reduced acceptor |
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Complex Reactions: | | | | | |
2.0 | + | 1.0 | + | 1.0Menaquinone 8 | → | 1.0 | + | 2.0 |
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Metabolites: | |
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GO Classification: | Function |
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binding | catalytic activity | cation binding | ferredoxin hydrogenase activity | ion binding | metal ion binding | nickel ion binding | oxidoreductase activity | oxidoreductase activity, acting on hydrogen as donor | oxidoreductase activity, acting on hydrogen as donor, iron-sulfur protein as acceptor | transition metal ion binding | Process |
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metabolic process | oxidation reduction |
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Gene Properties |
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Blattner: | b2994 |
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Gene Orientation | Counterclockwise |
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Centisome Percentage: | 67.66 |
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Left Sequence End | 3139308 |
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Right Sequence End | 3141011 |
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Gene Sequence: | >1704 bp
ATGGCTTTTGTATCAGAAAGAGAAATTGTAAGAAAGATATTTAGCAAAAAAATTGACTTT
ACGATCTTGGCATTTTTCTATATTTCCTCTATTTTTTTTCTTCTTTGTAGTGGCGTTCTC
TTTCAATATTTTACCGCTGCCTTCACAAAAGGTAATTGTTATGAATGCTCAATGAAACTT
GATTACATAAAACAATTTTATTTTTCACTGGAAACCGCATGGTACCTCATATCTGCTGTT
GCAGTATTTATTGCATCTGTTTTTATACAGCATAGAATTAAAGCTTATTTAACATTATTA
GCTATTACATGGATTGTACTAACAATAACAGATGTGGCGTTAATACACGCCTTAGACAAT
ATAGCCATGAATAATATTTTGTTAAATATACTATATAATCTTTTTGGGGCGATTTTATTG
TCACTGTTTATGTGTTTATCAAATTCCCTCCTTTTCCACCTTAATAAGATAAAACATATT
CCAATGATTTTATCGGCAATGATTCCATTAGTTTCCGCAATAATAATAGCCATACTCATA
ACCGCTGTTATATATTTATTGTTTGCCCGGCAGGCGGTAGAGATTGAGATGGATATATCT
GAAGGTTCAGACATCGCATATGTAGGTGTAAAAGATAATGAGGAATCATTTGGGTTTTTA
AATGATAAAAAAACAGACACACCAACGTATCTGGATGTAATCAAAAATGGTAGTTTGATA
TATAATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCC
CTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCTTTTCTGAATGTAAAA
AAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGAATCCGCTGAT
GTGATGCCTAAAGCAGCATCCAGGTTAAGTTTGAGCAAGCAAGATGATAAATTTATGCTA
GCCTCAAGTGTTACTGACTCTCAAATAAAATTTAAATCAAACAATGCACAATTGATGGTT
GCTTTCGCATTTATGCCAATAACAACGAATGGTATTTTACATGATTATACATACGATATA
ATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGAT
AAAAATAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAAC
ATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAGATGATATTATTAAT
TACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATAAAAAAACATAC
CAGAAGCTTGATAAAATATATGATGACATAAGCAATGGAAAATTATCTTCACTGAGAGCA
ACAGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAG
ATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATA
TATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACT
TATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATA
TTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGAT
GTGAATGAAAATTTATTCTTGTAA |
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Protein Properties |
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Pfam Domain Function: | |
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Protein Residues: | 567 |
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Protein Molecular Weight: | 62491 |
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Protein Theoretical pI: | 6 |
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Signaling Regions: | |
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Transmembrane Regions: | |
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Protein Sequence: | >Hydrogenase-2 large chain
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
SALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLP
PEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFP
GGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDG
KYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHS
TLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTI
HSFDPCMACAVHVVDADGNEVVSVKVL |
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References |
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External Links: | |
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General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Menon, N. K., Chatelus, C. Y., Dervartanian, M., Wendt, J. C., Shanmugam, K. T., Peck, H. D. Jr, Przybyla, A. E. (1994). "Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2." J Bacteriol 176:4416-4423. Pubmed: 8021226
- Sargent, F., Ballantine, S. P., Rugman, P. A., Palmer, T., Boxer, D. H. (1998). "Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit--identification of a soluble precursor of the small subunit in a hypB mutant." Eur J Biochem 255:746-754. Pubmed: 9738917
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