Hydrogenase-1 large chain (P0ACD8)

Identification
Name:Hydrogenase-1 large chain
Synonyms:
  • HYD1
  • Membrane-bound hydrogenase 1 large subunit
  • NiFe hydrogenase
Gene Name:hyaB
Enzyme Class:
Biological Properties
General Function:Involved in nickel cation binding
Specific Function:This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth
Cellular Location:Cell membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
  • Microbial metabolism in diverse environments ec01120
  • Trinitrotoluene degradation ec00633
KEGG Reactions:
1.02,4-Diamino-6-nitrotoluene ↔ 1.02,4-Diamino-6-hydroxylaminotoluene
1.02,4-Diamino-6-nitrotoluene ↔ 1.02,4-Diamino-6-hydroxylaminotoluene
ReactionCard
1.0Thumb+1.0Acceptor ↔ 1.0Reduced acceptor
1.0Hydrogen (gas) + 1.0Acceptor ↔ 1.0Reduced acceptor
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Electron+2.0Thumb+1.0menaquinone-8 → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Electron + 2.0Hydrogen ion + 1.0menaquinone-8 → 1.0Menaquinol 8 + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Ubiquinone-8 → 1.0Ubiquinol-8 + 2.0Hydrogen ion
ReactionCard
1.0Thumb+2.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
1.02-Demethylmenaquinone 8 + 2.0Hydrogen ion + 1.0Hydrogen (gas) → 1.02-Demethylmenaquinol 8 + 2.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb+1.0Menaquinone 8 → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Menaquinone 8 → 1.0Menaquinol 8 + 2.0Hydrogen ion
ReactionCard
1.0Thumb+1.0A → 1.0AH(2)
1.0Hydrogen (gas) + 1.0A → 1.0AH(2)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ferredoxin hydrogenase activity
ion binding
metal ion binding
nickel ion binding
oxidoreductase activity
oxidoreductase activity, acting on hydrogen as donor
oxidoreductase activity, acting on hydrogen as donor, iron-sulfur protein as acceptor
transition metal ion binding
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b0973
Gene OrientationClockwise
Centisome Percentage:22.25
Left Sequence End1032477
Right Sequence End1034270
Gene Sequence:
>1794 bp
ATGAGCACTCAGTACGAAACTCAGGGATACACCATCAATAATGCCGGACGCCGCCTGGTG
GTCGACCCGATTACGCGCATCGAAGGCCACATGCGCTGCGAAGTGAATATTAACGATCAG
AATGTGATCACCAATGCCGTCTCCTGCGGCACCATGTTTCGCGGGCTGGAGATCATCCTA
CAAGGGCGCGACCCGCGCGATGCGTGGGCGTTCGTTGAACGTATCTGCGGCGTCTGTACT
GGCGTACACGCCCTGGCTTCGGTTTACGCCATCGAAGATGCTATCGGTATTAAAGTGCCG
GACAACGCCAATATCATCCGCAACATTATGCTGGCAACGCTCTGGTGCCACGATCATCTG
GTGCACTTCTATCAGCTTGCCGGGATGGACTGGATCGATGTGTTAGATGCGCTGAAAGCC
GACCCGCGGAAAACCTCCGAACTGGCGCAAAGTCTCTCCTCTTGGCCGAAATCATCCCCT
GGCTATTTCTTCGACGTACAAAACCGCCTGAAAAAATTTGTTGAAGGCGGGCAGTTGGGG
ATCTTCCGCAATGGCTACTGGGGGCACCCGCAGTACAAACTGCCGCCAGAAGCTAACCTG
ATGGGCTTTGCCCACTATCTCGAAGCTCTCGATTTCCAGCGTGAAATTGTCAAAATCCAC
GCGGTCTTTGGCGGTAAAAACCCGCATCCAAACTGGATTGTCGGCGGGATGCCTTGCGCC
ATCAACATTGACGAAAGCGGCGCGGTCGGGGCAGTCAATATGGAACGCCTGAACCTGGTG
CAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTA
GCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTCTGATAAATGC
GTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTG
ATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTT
GATCCGCAGCAGGTGCAGGAGTTTGTCGACCACGCCTGGTATCGATATCCCAACGATCAG
GTCGGGCGTCATCCGTTCGATGGCATCACCGACCCGTGGTACAACCCCGGCGATGTCAAA
GGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTACTCGTGGATCAAAGCG
CCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTAT
CACAAAGGCGATGCTGCGACCGTTGAGTCGGTCGATCGCATGATGTCGGCGTTGAACCTG
CCGCTTTCCGGTATCCAGTCAACGTTAGGCCGCATTTTGTGCCGCGCGCACGAAGCGCAG
TGGGCCGCAGGTAAGTTGCAGTATTTCTTCGACAAGCTGATGACCAACCTGAAAAACGGC
AATCTCGCCACTGCTTCCACGGAAAAATGGGAACCTGCAACCTGGCCGACAGAGTGCCGT
GGTGTCGGTTTTACCGAAGCGCCGCGCGGGGCGTTAGGCCACTGGGCCGCCATTCGCGAT
GGCAAGATTGATCTCTACCAGTGCGTGGTGCCGACCACCTGGAACGCCAGCCCGCGCGAT
CCCAAAGGGCAGATTGGCGCTTATGAAGCGGCGCTGATGAACACCAAAATGGCGATCCCC
GAGCAACCGCTGGAGATCCTGCGTACTCTGCACAGCTTTGACCCGTGCCTCGCCTGTTCA
ACACACGTGCTGGGCGACGACGGTAGCGAGCTGATCTCCGTGCAGGTGCGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:597
Protein Molecular Weight:66253
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Hydrogenase-1 large chain
MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVITNAVSCGTMFRGLEIIL
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHL
VHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPGYFFDVQNRLKKFVEGGQLG
IFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCA
INIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKC
VLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYRYPNDQ
VGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAPRWRGNAMEVGPLARTLIAY
HKGDAATVESVDRMMSALNLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNG
NLATASTEKWEPATWPTECRGVGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRD
PKGQIGAYEAALMNTKMAIPEQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR
References
External Links:
ResourceLink
Uniprot ID:P0ACD8
Uniprot Name:MBHL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674807
Ecogene ID:EG10469
Ecocyc:EG10469
ColiBase:b0973
Kegg Gene:b0973
EchoBASE ID:EB0464
CCDB:MBHL_ECOLI
BacMap:16128939
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Menon, N. K., Robbins, J., Peck, H. D. Jr, Chatelus, C. Y., Choi, E. S., Przybyla, A. E. (1990). "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames." J Bacteriol 172:1969-1977. Pubmed: 2180913
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232