ECMDB: 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit (P0ABW0)

Identification

Name:

3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit

Synonyms:

Digoxigenin ferredoxin subunit

Gene Name:

hcaC

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in oxidoreductase activity

Specific Function:

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP- dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively. This protein seems to be a 2Fe-2S ferredoxin

Cellular Location:

Not Available

SMPDB Pathways:

2-Oxopent-4-enoate metabolism PW001890
2-Oxopent-4-enoate metabolism 2 PW002035
Phenylalanine metabolism PW000921

KEGG Pathways:

Phenylalanine metabolism ec00360

KEGG Reactions:

1.0

→

1.0

1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0NADH + 1.0Oxygen → 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

1.04-Chlorobiphenyl

1.0

↔

1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl

1.0

1.04-Chlorobiphenyl + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl + 1.0NAD
ReactionCard

1.04-Chlorobiphenyl

1.0

↔

1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl

1.0

1.04-Chlorobiphenyl + 1.0Oxygen + 1.0NADPH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl + 1.0NADP
ReactionCard

1.0Biphenyl

1.0

↔

1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl

1.0

1.0Biphenyl + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl + 1.0NAD
ReactionCard

1.0Biphenyl

1.0

↔

1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl

1.0

1.0Biphenyl + 1.0Oxygen + 1.0NADPH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl + 1.0NADP
ReactionCard

1.0

↔

1.0

1.0Hydrocinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

↔

1.0

1.0trans-Cinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

→

1.0

1.0Hydrocinnamic acid + 1.0NADH + 1.0Oxygen → 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0Oxygen + 1.0NADH → 1.0Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0NADH + 1.0Oxygen → 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

↔

1.0

1.0Hydrocinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

→

1.0

1.0Hydrocinnamic acid + 1.0NADH + 1.0Oxygen → 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Hydrogen ion + 1.0NADH + 1.0Oxygen + 1.0Hydrocinnamic acid → 1.0Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21422	Cinnamic acid	MetaboCard
ECMDB20129	cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol	MetaboCard
ECMDB21320	Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol	MetaboCard
ECMDB20130	cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol	MetaboCard
ECMDB00764	Hydrocinnamic acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB00217	NADP	MetaboCard
ECMDB04111	NADPH	MetaboCard
ECMDB04124	Oxygen	MetaboCard
ECMDB00930	trans-Cinnamic acid	MetaboCard

GO Classification:

Function
2 iron, 2 sulfur cluster binding
binding
catalytic activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
Process
metabolic process
oxidation reduction

Gene Properties

Blattner:

b2540

Gene Orientation

Clockwise

Centisome Percentage:

57.52

Left Sequence End

2668930

Right Sequence End

2669250

Gene Sequence:

>321 bp
ATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTAACCACCGTCGGC
GCTGCCGAGAGAACTTTAACCCCACAACAACAGCGTATGACCTCCTGTAATCAGCAGGCG
ACGGCGCAGGCGTTGAAAGGGGATGCTCGTAAGACCTACATGAGTGATTGCCTGAAGAAC
AGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGC
AATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCC
TGCCTCAAGAAAGCCGCCTGA

Protein Properties

Pfam Domain Function:

Rieske (PF00355 )

Protein Residues:

106

Protein Molecular Weight:

11329

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit
MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSHGNASMSEGYLEDDATV
ECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLPEAQP

References

External Links:

Resource	Link
Uniprot ID:	P0ABW0
Uniprot Name:	HCAC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674525
Ecogene ID:	EG13458
Ecocyc:	EG13458
ColiBase:	b2540
Kegg Gene:	b2540
EchoBASE ID:	EB3231
CCDB:	HCAC_ECOLI
BacMap:	16130465

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Diaz, E., Ferrandez, A., Garcia, J. L. (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." J Bacteriol 180:2915-2923. Pubmed: 9603882
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837