Arsenate reductase (P0AB96)

Identification
Name:Arsenate reductase
Synonyms:
  • Arsenical pump modifier
Gene Name:arsC
Enzyme Class:
Biological Properties
General Function:Involved in arsenate reductase (glutaredoxin) activity
Specific Function:Reduction of arsenate [As(V)] to arsenite [As(III)]. This protein expands the substrate specificity of ArsAB pump which can extrude arsenite and antimonite to allow for arsenate pumping and resistance
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Glutaredoxin ↔ 1.0Thumb+1.0Glutaredoxin disulfide+1.0Thumb
1.0Arsenate ion + 1.0Glutaredoxin ↔ 1.0Arsenite + 1.0Glutaredoxin disulfide + 1.0Water
ReactionCard
Complex Reactions:
1.0Thumb+2.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Arsenate + 2.0Glutathione → 1.0Arsenite + 1.0Glutathione disulfide + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21394ArsenateMetaboCard
ECMDB21309ArseniteMetaboCard
ECMDB00125GlutathioneMetaboCard
ECMDB21221Glutathione disulfideMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
arsenate reductase (glutaredoxin) activity
arsenate reductase activity
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b3503
Gene OrientationClockwise
Centisome Percentage:78.63
Left Sequence End3648260
Right Sequence End3648685
Gene Sequence:
>426 bp
GTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAAC
CGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCAT
GGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGA
TACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGC
CAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCAT
CTTGAGCGACAGTTCGCCGTAACGGAACCAAATCAGGTGTGGTGCGGTGATGTGACCTAT
AGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAA
TATTGA
Protein Properties
Pfam Domain Function:
Protein Residues:141
Protein Molecular Weight:15853
Protein Theoretical pI:5
PDB File:1I9D
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Arsenate reductase
MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDELVKLIADMGISVRALLR
KNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLGTRLCRPSEVVLEILPDAQ
KGAFSKEDGEKVVDEAGKRLK
References
External Links:
ResourceLink
Uniprot ID:P0AB96
Uniprot Name:ARSC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674401
PDB ID:1I9D
Ecogene ID:EG12237
Ecocyc:EG12237
ColiBase:b3503
Kegg Gene:b3503
EchoBASE ID:EB2149
CCDB:ARSC_ECOLI
BacMap:16131375
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Diorio, C., Cai, J., Marmor, J., Shinder, R., DuBow, M. S. (1995). "An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria." J Bacteriol 177:2050-2056. Pubmed: 7721697
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620