NAD(P) transhydrogenase subunit beta (P0AB67)

Identification
Name:NAD(P) transhydrogenase subunit beta
Synonyms:
  • Nicotinamide nucleotide transhydrogenase subunit beta
  • Pyridine nucleotide transhydrogenase subunit beta
Gene Name:pntB
Enzyme Class:
Biological Properties
General Function:Involved in NAD(P)+ transhydrogenase (AB-specific) activity
Specific Function:The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • NAD phosphorylation and dephosphorylation PW002081
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NAD + 1.0NADPH ↔ 1.0NADH + 1.0NADP
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADH + 1.0NADP → 1.0NADPH + 1.0NAD
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NAD + 1.0NADPH ↔ 1.0NADH + 1.0NADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+2.0Thumb → 2.0Thumb+1.0Thumb+1.0Thumb
1.0NADH + 1.0NADP + 2.0Hydrogen ion → 2.0Hydrogen ion + 1.0NAD + 1.0NADPH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
binding
catalytic activity
NAD(P)+ transhydrogenase (AB-specific) activity
NAD(P)+ transhydrogenase activity
NADP or NADPH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
oxidoreductase activity, acting on NADH or NADPH, NAD or NADP as acceptor
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b1602
Gene OrientationCounterclockwise
Centisome Percentage:36.06
Left Sequence End1672996
Right Sequence End1674384
Gene Sequence:
>1389 bp
ATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCGATCCTGTTTATCTTCAGT
CTGGCCGGTCTTTCGAAACATGAAACGTCTCGCCAGGGTAACAACTTCGGTATCGCCGGG
ATGGCGATTGCGTTAATCGCAACCATTTTTGGACCGGATACGGGTAATGTTGGCTGGATC
TTGCTGGCGATGGTCATTGGTGGGGCAATTGGTATCCGTCTGGCGAAGAAAGTTGAAATG
ACCGAAATGCCAGAACTGGTGGCGATCCTGCATAGCTTCGTGGGTCTGGCGGCAGTGCTG
GTTGGCTTTAACAGCTATCTGCATCATGACGCGGGAATGGCACCGATTCTGGTCAATATT
CACCTGACGGAAGTGTTCCTCGGTATCTTCATCGGGGCGGTAACGTTCACGGGTTCGGTG
GTGGCGTTCGGCAAACTGTGTGGCAAGATTTCGTCTAAACCATTGATGCTGCCAAACCGT
CACAAAATGAACCTGGCGGCTCTGGTCGTTTCCTTCCTGCTGCTGATTGTATTTGTTCGC
ACGGACAGCGTCGGCCTGCAAGTGCTGGCATTGCTGATAATGACCGCAATTGCGCTGGTA
TTCGGCTGGCATTTAGTCGCCTCCATCGGTGGTGCAGATATGCCAGTGGTGGTGTCGATG
CTGAACTCGTACTCCGGCTGGGCGGCTGCGGCTGCGGGCTTTATGCTCAGCAACGACCTG
CTGATTGTGACCGGTGCGCTGGTCGGTTCTTCGGGGGCTATCCTTTCTTACATTATGTGT
AAGGCGATGAACCGTTCCTTTATCAGCGTTATTGCGGGTGGTTTCGGCACCGACGGCTCT
TCTACTGGCGATGATCAGGAAGTGGGTGAGCACCGCGAAATCACCGCAGAAGAGACAGCG
GAACTGCTGAAAAACTCCCATTCAGTGATCATTACTCCGGGGTACGGCATGGCAGTCGCG
CAGGCGCAATATCCTGTCGCTGAAATTACTGAGAAATTGCGCGCTCGTGGTATTAATGTG
CGTTTCGGTATCCACCCGGTCGCGGGGCGTTTGCCTGGACATATGAACGTATTGCTGGCT
GAAGCAAAAGTACCGTATGACATCGTGCTGGAAATGGACGAGATCAATGATGACTTTGCT
GATACCGATACCGTACTGGTGATTGGTGCTAACGATACGGTTAACCCGGCGGCGCAGGAT
GATCCGAAGAGTCCGATTGCTGGTATGCCTGTGCTGGAAGTGTGGAAAGCGCAGAACGTG
ATTGTCTTTAAACGTTCGATGAACACTGGCTATGCTGGTGTGCAAAACCCGCTGTTCTTC
AAGGAAAACACCCACATGCTGTTTGGTGACGCCAAAGCCAGCGTGGATGCAATCCTGAAA
GCTCTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:462
Protein Molecular Weight:48723
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • 4-24
  • 46-66
  • 83-103
  • 116-136
  • 165-185
  • 189-209
  • 216-236
  • 240-260
  • 309-329
Protein Sequence:
>NAD(P) transhydrogenase subunit beta
MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWI
LLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNI
HLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRHKMNLAALVVSFLLLIVFVR
TDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETA
ELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLA
EAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDDPKSPIAGMPVLEVWKAQNV
IVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL
References
External Links:
ResourceLink
Uniprot ID:P0AB67
Uniprot Name:PNTB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742636
Ecogene ID:EG10745
Ecocyc:EG10745
ColiBase:b1602
Kegg Gene:b1602
EchoBASE ID:EB0738
CCDB:PNTB_ECOLI
BacMap:16129560
General Reference:
  • Ahmad, S., Glavas, N. A., Bragg, P. D. (1992). "A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change." Eur J Biochem 207:733-739. Pubmed: 1633824
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Clarke, D. M., Loo, T. W., Gillam, S., Bragg, P. D. (1986). "Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli." Eur J Biochem 158:647-653. Pubmed: 3525165
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Tong, R. C., Glavas, N. A., Bragg, P. D. (1991). "Topological analysis of the pyridine nucleotide transhydrogenase of Escherichia coli using proteolytic enzymes." Biochim Biophys Acta 1080:19-28. Pubmed: 1932078