Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit (P0AAJ3)

Identification
Name:Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit
Synonyms:
  • Anaerobic formate dehydrogenase iron-sulfur subunit
  • Formate dehydrogenase-N subunit beta
  • FDH-N subunit beta
Gene Name:fdnH
Enzyme Class:Not Available
Biological Properties
General Function:Involved in electron carrier activity
Specific Function:Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit
Cellular Location:Cell membrane; Single-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Formic acid + 1.0NAD ↔ 1.0Hydrogen ion + 1.0Carbon dioxide + 1.0NADH
ReactionCard
Complex Reactions:
2.0Thumb+1.0Menaquinone 8+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
2.0Hydrogen ion + 1.0Menaquinone 8 + 1.0Formic acid → 1.0Menaquinol 8 + 1.0Carbon dioxide + 1.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
2.0Hydrogen ion + 1.0Ubiquinone-8 + 1.0Formic acid → 1.0Ubiquinol-8 + 1.0Carbon dioxide + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
binding
catalytic activity
electron carrier activity
formate dehydrogenase activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Process
cellular metabolic process
cellular respiration
energy derivation by oxidation of organic compounds
generation of precursor metabolites and energy
metabolic process
Gene Properties
Blattner:b1475
Gene OrientationClockwise
Centisome Percentage:33.37
Left Sequence End1548485
Right Sequence End1549369
Gene Sequence:
>885 bp
ATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCT
TCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATC
GGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGG
CACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATG
CGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGT
ATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAG
TACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATT
GCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGC
ACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGTCCG
ACCGGGGCTATCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTG
GCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGT
GGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTG
CCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTG
GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGC
CCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:294
Protein Molecular Weight:32239
Protein Theoretical pI:6
PDB File:1KQG
Signaling Regions:
  • None
Transmembrane Regions:
  • 257-279
Protein Sequence:
>Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit
MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKACQVACSEWNDIRDEVG
HCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMHCEDPGCLKACPSAGAIIQ
YANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKCTLCVDRVSVGQEPACVKTCP
TGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEGVGGTHVMYVLHHADQPELYHGL
PKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHYIGIGPNKEVDDDEEDHHE
References
External Links:
ResourceLink
Uniprot ID:P0AAJ3
Uniprot Name:FDNH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742408
PDB ID:1KQG
Ecogene ID:EG11228
Ecocyc:EG11228
ColiBase:b1475
Kegg Gene:b1475
EchoBASE ID:EB1210
CCDB:FDNH_ECOLI
BacMap:16129434
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Berg, B. L., Li, J., Heider, J., Stewart, V. (1991). "Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine." J Biol Chem 266:22380-22385. Pubmed: 1834669
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jormakka, M., Tornroth, S., Byrne, B., Iwata, S. (2002). "Molecular basis of proton motive force generation: structure of formate dehydrogenase-N." Science 295:1863-1868. Pubmed: 11884747