Galactose/methyl galactoside import ATP-binding protein MglA (P0AAG8)

Identification
Name:Galactose/methyl galactoside import ATP-binding protein MglA
Synonyms:Not Available
Gene Name:mglA
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex MglABC involved in galactose/methyl galactoside import. Responsible for energy coupling to the transport system (Probable)
Cellular Location:Cell inner membrane; Peripheral membrane protein (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0D-Galactose → 1.0ADP + 1.0D-Galactose + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0D-Glucose → 1.0ADP + 1.0D-Glucose + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00143D-GalactoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
carbohydrate transmembrane transporter activity
carbohydrate-transporting ATPase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
transport
Gene Properties
Blattner:b2149
Gene OrientationCounterclockwise
Centisome Percentage:48.19
Left Sequence End2235791
Right Sequence End2237311
Gene Sequence:
>1521 bp
ATGGTCAGCTCAACGACTCCGTCCTCCGGGGAATACTTGTTGGAAATGAGCGGTATCAAC
AAGTCCTTTCCTGGTGTTAAGGCACTTGATAACGTTAATTTAAAAGTCCGGCCACATTCT
ATCCATGCATTAATGGGGGAAAACGGTGCAGGAAAATCGACATTATTAAAATGCCTGTTT
GGTATTTATCAAAAAGACTCCGGCACCATTTTATTCCAGGGTAAAGAGATCGATTTCCAT
TCTGCAAAAGAAGCCCTGGAAAATGGTATTTCGATGGTACACCAGGAGTTAAACCTGGTA
TTACAACGTTCGGTGATGGATAACATGTGGCTGGGGCGATATCCCACCAAAGGCATGTTT
GTCGATCAGGACAAAATGTACCGCGAAACCAAAGCGATTTTTGACGAACTGGATATTGAT
ATCGATCCGCGTGCGCGCGTCGGCACATTATCCGTTTCGCAAATGCAGATGATCGAAATC
GCCAAAGCGTTTTCCTATAACGCGAAAATTGTGATTATGGATGAACCGACTTCTTCGTTA
ACCGAAAAAGAGGTCAATCATCTGTTCACTATTATTCGTAAATTAAAAGAGCGCGGCTGC
GGTATTGTTTATATCTCGCATAAAATGGAAGAAATCTTCCAGTTATGTGATGAAGTTACC
GTATTGCGCGACGGTCAGTGGATCGCCACCGAACCGCTGGCAGGACTGACGATGGACAAG
ATCATCGCCATGATGGTTGGGCGTTCTCTTAACCAGCGTTTCCCTGACAAAGAAAACAAA
CCGGGCGAAGTCATCCTCGAGGTACGTAACCTGACGTCACTGCGCCAGCCGTCGATTCGC
GATGTCTCGTTTGATCTGCATAAAGGGGAGATCCTCGGTATTGCCGGTCTGGTGGGGGCG
AAACGTACCGATATTGTTGAGACGTTATTTGGTATTCGCGAGAAATCGGCTGGCACCATA
ACGTTGCACGGCAAACAGATCAATAACCATAATGCCAACGAAGCCATAAACCATGGATTT
GCACTGGTAACTGAGGAGCGCCGCTCAACGGGAATTTATGCCTATCTGGATATTGGTTTT
AACTCGTTAATTTCCAATATTCGCAACTACAAAAATAAAGTTGGTTTACTGGATAACTCG
CGGATGAAAAGCGATACCCAGTGGGTGATTGATTCGATGCGGGTAAAAACGCCGGGTCAT
CGGACGCAAATTGGTTCGCTCTCCGGTGGTAATCAGCAAAAGGTGATTATTGGTCGCTGG
CTACTAACGCAACCAGAAATATTAATGCTCGATGAACCGACGCGCGGTATTGATGTCGGG
GCGAAGTTTGAAATTTATCAGTTAATTGCCGAACTGGCGAAGAAAGGCAAGGGGATTATT
ATTATCTCCTCTGAAATGCCTGAGTTGTTAGGGATAACAGACCGTATTCTGGTCATGAGC
AATGGTCTCGTTTCCGGAATTGTCGATACAAAAACAACAACGCAAAACGAAATTCTGCGT
CTTGCGTCTTTGCACCTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:506
Protein Molecular Weight:56415
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Galactose/methyl galactoside import ATP-binding protein MglA
MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLF
GIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQRSVMDNMWLGRYPTKGMF
VDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSL
TEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMDK
IIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQPSIRDVSFDLHKGEILGIAGLVGA
KRTDIVETLFGIREKSAGTITLHGKQINNHNANEAINHGFALVTEERRSTGIYAYLDIGF
NSLISNIRNYKNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSLSGGNQQKVIIGRW
LLTQPEILMLDEPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMPELLGITDRILVMS
NGLVSGIVDTKTTTQNEILRLASLHL
References
External Links:
ResourceLink
Uniprot ID:P0AAG8
Uniprot Name:MGLA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675263
Ecogene ID:EG10592
Ecocyc:EG10592
ColiBase:b2149
Kegg Gene:b2149
EchoBASE ID:EB0587
CCDB:MGLA_ECOLI
BacMap:16130087
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Boos, W., Sarvas, M. O. (1970). "Close linkage between a galactose binding protein and the beta-methylgalactoside permease in Escherichia coli." Eur J Biochem 13:526-533. Pubmed: 4910389
  • Harayama, S., Bollinger, J., Iino, T., Hazelbauer, G. L. (1983). "Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning." J Bacteriol 153:408-415. Pubmed: 6294056
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hogg, R. W., Voelker, C., Von Carlowitz, I. (1991). "Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12." Mol Gen Genet 229:453-459. Pubmed: 1719366
  • Rotman, B., Guzman, R. (1982). "Identification of the mglA gene product in the beta-methylgalactoside transport system of Escherichia coli using plasmid DNA deletions generated in vitro." J Biol Chem 257:9030-9034. Pubmed: 6807987
  • Scholle, A., Vreemann, J., Blank, V., Nold, A., Boos, W., Manson, M. D. (1987). "Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors." Mol Gen Genet 208:247-253. Pubmed: 3302609