D-3-phosphoglycerate dehydrogenase (P0A9T0)

Identification
Name:D-3-phosphoglycerate dehydrogenase
Synonyms:
  • PGDH
Gene Name:serA
Enzyme Class:
Biological Properties
General Function:Involved in amino acid binding
Specific Function:3-phospho-D-glycerate + NAD(+) = 3- phosphonooxypyruvate + NADH
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: cysteine biosynthesis from serine PW000977
  • cysteine biosynthesis PW000800
  • serine biosynthesis and metabolism PW000809
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Phospho-D-glycerate + 1.0NAD ↔ 1.0Phosphohydroxypyruvic acid + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Phospho-D-glycerate + 1.0NAD → 1.0Phosphohydroxypyruvic acid + 1.0NADH
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.03-Phospho-D-glycerate + 1.0NAD + 1.0D-2-Hydroxyglutaric acid ↔ 1.0Phosphohydroxypyruvic acid + 1.0NADH + 1.0Hydrogen ion + 1.0alpha-Ketoglutarate
ReactionCard
SMPDB Reactions:
1.03-Phosphoglyceric acid+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Phosphoglyceric acid + 1.0NAD + 1.03-Phosphoglycerate → 1.0NADH + 1.0Hydrogen ion + 1.0Phosphohydroxypyruvic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Phosphoglycerate + 1.0NAD → 1.0Phosphohydroxypyruvic acid + 1.0Hydrogen ion + 1.0NADH
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0D-2-Hydroxyglutaric acid + 1.0NAD → 1.0Oxoglutaric acid + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB216453-Phospho-D-glycerateMetaboCard
ECMDB041313-PhosphoglycerateMetaboCard
ECMDB00606D-2-Hydroxyglutaric acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB04123Oxoglutaric acidMetaboCard
ECMDB01024Phosphohydroxypyruvic acidMetaboCard
GO Classification:
Function
amino acid binding
binding
carboxylic acid binding
catalytic activity
cofactor binding
NAD or NADH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
metabolic process
Gene Properties
Blattner:b2913
Gene OrientationCounterclockwise
Centisome Percentage:65.85
Left Sequence End3055200
Right Sequence End3056432
Gene Sequence:
>1233 bp
ATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTC
TGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATG
TTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCG
TATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGT
CGCCGTCCGGTGATGCTGGCGGGAGTGGTGTGGTTTATCGTCACCTGTCTGGCAATATTG
CTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGT
TTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATC
AAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTG
GGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTG
GCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGC
GAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGC
TTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCC
CAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTG
CAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCG
CGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTG
GTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTA
AGTATTTATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTT
GCCAGCGATATGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATC
TTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTT
AATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAA
GATAAACAGATGGGAAATTCTCACGAAGGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:410
Protein Molecular Weight:44175
Protein Theoretical pI:6
PDB File:1PSD
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>D-3-phosphoglycerate dehydrogenase
MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDDEQLKESIRDAHFIGLR
SRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVFNAPFSNTRSVAELVIGEL
LLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYGHIGTQLGILAESLGMYVYFY
DIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTKNMMGAKEISLMKPGSLLINASR
GTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDPFTSPLCEFDNVLLTPHIGGSTQEA
QENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPLHGGRRLMHIHENRPGVLTALNKIFAE
QGVNIAAQYLQTSAQMGYVVIDIEADEDVAEKALQAMKAIPGTIRARLLY
References
External Links:
ResourceLink
Uniprot ID:P0A9T0
Uniprot Name:SERA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062426
PDB ID:1PSD
Ecogene ID:EG10944
Ecocyc:EG10944
ColiBase:b2913
Kegg Gene:b2913
EchoBASE ID:EB0937
CCDB:SERA_ECOLI
BacMap:16130814
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Rex, J. H., Aronson, B. D., Somerville, R. L. (1991). "The tdh and serA operons of Escherichia coli: mutational analysis of the regulatory elements of leucine-responsive genes." J Bacteriol 173:5944-5953. Pubmed: 1917830
  • Schuller, D. J., Grant, G. A., Banaszak, L. J. (1995). "The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase." Nat Struct Biol 2:69-76. Pubmed: 7719856
  • Tobey, K. L., Grant, G. A. (1986). "The nucleotide sequence of the serA gene of Escherichia coli and the amino acid sequence of the encoded protein, D-3-phosphoglycerate dehydrogenase." J Biol Chem 261:12179-12183. Pubmed: 3017965