ECMDB

Identification

Name:

Hypoxanthine phosphoribosyltransferase

Synonyms:

HPRT

Gene Name:

hpt

Enzyme Class:

2.4.2.8

Biological Properties

General Function:

Involved in hypoxanthine phosphoribosyltransferase activity

Specific Function:

This enzyme acts exclusively on hypoxanthine; it does not act on guanine

Cellular Location:

Cytoplasm

SMPDB Pathways:

Not Available

KEGG Pathways:

Drug metabolism - other enzymes ec00983
Metabolic pathways eco01100
Purine metabolism ec00230

KEGG Reactions:

1.0

→

1.0

1.0Guanine + 1.0Phosphoribosyl pyrophosphate → 1.0Guanosine monophosphate + 1.0Pyrophosphate
ReactionCard

1.0

↔

1.0

1.0Hypoxanthine + 1.0Phosphoribosyl pyrophosphate ↔ 1.0Inosinic acid + 1.0Pyrophosphate
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1.0

↔

1.0

1.0Phosphoribosyl pyrophosphate + 1.0Xanthine ↔ 1.0Pyrophosphate + 1.0Xanthylic acid
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1.0

↔

1.0

1.0Adenine + 1.0Phosphoribosyl pyrophosphate ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate
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1.0

↔

1.0

1.0Adenosine monophosphate + 1.0Pyrophosphate ↔ 1.0Adenine + 1.0Phosphoribosyl pyrophosphate
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1.0

↔

1.0

1.0Inosinic acid + 1.0Pyrophosphate ↔ 1.0Hypoxanthine + 1.0Phosphoribosyl pyrophosphate
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1.0

↔

1.0

1.0Guanosine monophosphate + 1.0Pyrophosphate ↔ 1.0Guanine + 1.0Phosphoribosyl pyrophosphate
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1.0

↔

1.0

1.0Xanthylic acid + 1.0Pyrophosphate ↔ 1.0Xanthine + 1.0Phosphoribosyl pyrophosphate
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1.06-Mercaptopurine

1.0

↔

1.06-Thioinosine-5'-monophosphate

1.0

1.06-Mercaptopurine + 1.0Phosphoribosyl pyrophosphate ↔ 1.06-Thioinosine-5'-monophosphate + 1.0Pyrophosphate
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1.06-Methylmercaptopurine

1.0

↔

1.06-Methylthiopurine 5'-monophosphate ribonucleotide

1.0

1.06-Methylmercaptopurine + 1.0Phosphoribosyl pyrophosphate ↔ 1.06-Methylthiopurine 5'-monophosphate ribonucleotide + 1.0Pyrophosphate
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1.0Thioguanine

1.0

↔

1.06-Thioguanosine monophosphate

1.0

1.0Thioguanine + 1.0Phosphoribosyl pyrophosphate ↔ 1.06-Thioguanosine monophosphate + 1.0Pyrophosphate
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1.0

←

1.0

1.0Xanthylic acid + 1.0Pyrophosphate ← 1.0Xanthine + 1.0Phosphoribosyl pyrophosphate
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EcoCyc Reactions:

1.0

→

1.0

1.0Guanine + 1.0Phosphoribosyl pyrophosphate → 1.0Guanosine monophosphate + 1.0Pyrophosphate
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1.0

↔

1.0

1.0Hypoxanthine + 1.0Phosphoribosyl pyrophosphate ↔ 1.0Inosinic acid + 1.0Pyrophosphate
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1.0

↔

1.0

1.0Phosphoribosyl pyrophosphate + 1.0Xanthine ↔ 1.0Pyrophosphate + 1.0Xanthylic acid
ReactionCard

1.0

↔

1.0

1.0Adenine + 1.0Phosphoribosyl pyrophosphate ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate
ReactionCard

1.0

←

1.0

1.0Xanthylic acid + 1.0Pyrophosphate ← 1.0Xanthine + 1.0Phosphoribosyl pyrophosphate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Inosinic acid + 1.0Pyrophosphate → 1.0Hypoxanthine + 1.0Phosphoribosyl pyrophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00034	Adenine	MetaboCard
ECMDB00045	Adenosine monophosphate	MetaboCard
ECMDB00132	Guanine	MetaboCard
ECMDB01397	Guanosine monophosphate	MetaboCard
ECMDB00157	Hypoxanthine	MetaboCard
ECMDB04085	Inosinic acid	MetaboCard
ECMDB00280	Phosphoribosyl pyrophosphate	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard
ECMDB00292	Xanthine	MetaboCard
ECMDB01554	Xanthylic acid	MetaboCard

GO Classification:

Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
hypoxanthine phosphoribosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
Process
biosynthetic process
cellular biosynthetic process
cellular nitrogen compound metabolic process
heterocycle biosynthetic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
purine ribonucleoside salvage
purine salvage

Gene Properties

Blattner:

b0125

Gene Orientation

Clockwise

Centisome Percentage:

3.05

Left Sequence End

141431

Right Sequence End

141967

Gene Sequence:

>537 bp
ATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAA
CTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGT
CTGCTGCGTGGCTCATTTATGTTTATGGCGGACCTGTGCCGTGAAGTTCAGGTATCTCAT
GAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTG
AAAATCCTCAAAGATCTGGATGAAGATATCCGTGGCAAGGACGTGCTGATTGTTGAAGAT
ATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCG
AAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCG
GTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTAC
GCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAA

Protein Properties

Pfam Domain Function:

Pribosyltran (PF00156 )

Protein Residues:

178

Protein Molecular Weight:

20115

Protein Theoretical pI:

PDB File:

1GRV

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Hypoxanthine phosphoribosyltransferase
MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRGSFMFMADLCREVQVSH
EVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDIIDSGNTLSKVREILSLREP
KSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDYAQRYRHLPYIGKVILLDE

References

External Links:

Resource	Link
Uniprot ID:	P0A9M2
Uniprot Name:	HPRT_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674341
PDB ID:	1GRV
Ecogene ID:	EG20098
Ecocyc:	EG20098
ColiBase:	b0125
Kegg Gene:	b0125
EchoBASE ID:	EB4143
CCDB:	HPRT_ECOLI
BacMap:	90111088

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
Guddat, L. W., Vos, S., Martin, J. L., Keough, D. T., de Jersey, J. (2002). "Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase." Protein Sci 11:1626-1638. Pubmed: 12070315
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553

Hypoxanthine phosphoribosyltransferase (P0A9M2)