Ribokinase (P0A9J6)

Identification
Name:Ribokinase
Synonyms:Not Available
Gene Name:rbsK
Enzyme Class:
Biological Properties
General Function:Involved in phosphotransferase activity, alcohol group as acceptor
Specific Function:ATP + D-ribose = ADP + D-ribose 5-phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Ribose ↔ 1.0ADP + 1.0Hydrogen ion + 1.0D-Ribose-5-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Ribose ↔ 1.0ADP + 1.0D-Ribose-5-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Deoxyribose 5-phosphate + 1.0ADP ↔ 1.0Deoxyribose + 1.0Adenosine triphosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Ribose + 1.0Adenosine triphosphate → 1.0D-Ribose-5-phosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Ribose → 1.0ADP + 1.0D-Ribose-5-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB02033D-Ribose-5-phosphateMetaboCard
ECMDB03224DeoxyriboseMetaboCard
ECMDB01031Deoxyribose 5-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00283RiboseMetaboCard
GO Classification:
Function
catalytic activity
phosphotransferase activity, alcohol group as acceptor
ribokinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
alcohol metabolic process
D-ribose metabolic process
metabolic process
monosaccharide metabolic process
pentose metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3752
Gene OrientationClockwise
Centisome Percentage:84.82
Left Sequence End3935317
Right Sequence End3936246
Gene Sequence:
>930 bp
ATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCT
GTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATC
GACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTC
TCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGAT
CGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTC
AGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGG
AAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTG
CCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTG
AAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTC
GCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTC
TGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCG
GCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGG
GACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAG
CTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTC
TCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAA
ACCCTGCTGCAACAGCTCAAGCTTTGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:309
Protein Molecular Weight:32290
Protein Theoretical pI:5
PDB File:1GQT
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribokinase
MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKGANQAVAAGRSGANIAF
IACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVNGEGENVIGIHAGANAAL
SPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPARELPDELLAL
VDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIRTVLITLGSRGVWASVNGEGQRV
PGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIRFAHAAAAIAVTRKGAQPSVPWREE
IDAFLDRQR
References
External Links:
ResourceLink
Uniprot ID:P0A9J6
Uniprot Name:RBSK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674412
PDB ID:1GQT
Ecogene ID:EG10818
Ecocyc:EG10818
ColiBase:b3752
Kegg Gene:b3752
EchoBASE ID:EB0811
CCDB:RBSK_ECOLI
BacMap:16131620
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hope, J. N., Bell, A. W., Hermodson, M. A., Groarke, J. M. (1986). "Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase." J Biol Chem 261:7663-7668. Pubmed: 3011794
  • Sigrell, J. A., Cameron, A. D., Jones, T. A., Mowbray, S. L. (1997). "Purification, characterization, and crystallization of Escherichia coli ribokinase." Protein Sci 6:2474-2476. Pubmed: 9385653
  • Sigrell, J. A., Cameron, A. D., Jones, T. A., Mowbray, S. L. (1998). "Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8 A resolution: insights into a new family of kinase structures." Structure 6:183-193. Pubmed: 9519409
  • Sigrell, J. A., Cameron, A. D., Mowbray, S. L. (1999). "Induced fit on sugar binding activates ribokinase." J Mol Biol 290:1009-1018. Pubmed: 10438599