KHG/KDPG aldolase (P0A955)

Identification
Name:KHG/KDPG aldolase
Synonyms:
  • 4-hydroxy-2-oxoglutarate aldolase
  • 2-keto-4-hydroxyglutarate aldolase
  • KHG-aldolase
  • 2-dehydro-3-deoxy-phosphogluconate aldolase
  • 2-keto-3-deoxy-6-phosphogluconate aldolase
  • KDPG-aldolase
  • Phospho-2-dehydro-3-deoxygluconate aldolase
  • Phospho-2-keto-3-deoxygluconate aldolase
Gene Name:eda
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Keto-3-deoxy-6-phosphogluconic acid ↔ 1.0D-Glyceraldehyde 3-phosphate + 1.0Pyruvic acid
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1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.04-Hydroxy-2-oxoglutaric acid ↔ 1.0Pyruvic acid + 1.0Glyoxylic acid
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1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-4-Hydroxy-2-oxoglutarate ↔ 1.0Pyruvic acid + 1.0Glyoxylic acid
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SMPDB Reactions:
1.0Thumb → 1.0D-Glyceraldehyde 3-phosphate+1.0Thumb+1.0Thumb
1.02-Keto-3-deoxy-6-phosphogluconic acid → 1.0D-Glyceraldehyde 3-phosphate + 1.0Pyruvic acid + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Keto-3-deoxy-6-phosphogluconic acid ↔ 1.0D-Glyceraldehyde 3-phosphate + 1.0Pyruvic acid
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1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Oxalacetic acid → 1.0Carbon dioxide + 1.0Pyruvic acid
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1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.04-Hydroxy-2-oxoglutaric acid ↔ 1.0Pyruvic acid + 1.0Glyoxylic acid
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.04-Hydroxy-2-oxoglutaric acid → 1.0Pyruvic acid + 1.0Glyoxylic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB013762-Keto-3-deoxy-6-phosphogluconic acidMetaboCard
ECMDB020704-Hydroxy-2-oxoglutaric acidMetaboCard
ECMDB04030Carbon dioxideMetaboCard
ECMDB20135D-4-Hydroxy-2-oxoglutarateMetaboCard
ECMDB01112D-Glyceraldehyde 3-phosphateMetaboCard
ECMDB00119Glyoxylic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00223Oxalacetic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Function
catalytic activity
Process
metabolic process
Gene Properties
Blattner:b1850
Gene OrientationCounterclockwise
Centisome Percentage:41.60
Left Sequence End1930139
Right Sequence End1930780
Gene Sequence:
>642 bp
ATGCGCAGTAAGTATATCGTCATTGAGGGGCTGGAAGGCGCAGGCAAAACTACCGCGCGT
AATGTGGTGGTTGAGACGCTCGAGCAACTGGGTATCCGCGACATGGTTTTCACTCGGGAA
CCTGGCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTA
GGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAA
CTGGTAGAAACGGTCATCAAACCAGCTCTGGCTAACGGCACCTGGGTGATTGGCGATCGC
CACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTG
GCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGAT
GTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAG
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAA
GATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATC
CGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:213
Protein Molecular Weight:22284
Protein Theoretical pI:5
PDB File:1FQ0
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>KHG/KDPG aldolase
MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAI
AKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSE
LMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLC
IGGSWLVPADALEAGDYDRITKLAREAVEGAKL
References
External Links:
ResourceLink
Uniprot ID:P0A955
Uniprot Name:ALKH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062665
PDB ID:1FQ0
Ecogene ID:EG10256
Ecocyc:EG10256
ColiBase:b1850
Kegg Gene:b1850
EchoBASE ID:EB0252
CCDB:ALKH_ECOLI
BacMap:16129803
General Reference:
  • Allard, J., Grochulski, P., Sygusch, J. (2001). "Covalent intermediate trapped in 2-keto-3-deoxy-6- phosphogluconate (KDPG) aldolase structure at 1.95-A resolution." Proc Natl Acad Sci U S A 98:3679-3684. Pubmed: 11274385
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carter, A. T., Pearson, B. M., Dickinson, J. R., Lancashire, W. E. (1993). "Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway." Gene 130:155-156. Pubmed: 8344525
  • Egan, S. E., Fliege, R., Tong, S., Shibata, A., Wolf, R. E. Jr, Conway, T. (1992). "Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon." J Bacteriol 174:4638-4646. Pubmed: 1624451
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Patil, R. V., Dekker, E. E. (1992). "Cloning, nucleotide sequence, overexpression, and inactivation of the Escherichia coli 2-keto-4-hydroxyglutarate aldolase gene." J Bacteriol 174:102-107. Pubmed: 1339418
  • Vlahos, C. J., Dekker, E. E. (1988). "The complete amino acid sequence and identification of the active-site arginine peptide of Escherichia coli 2-keto-4-hydroxyglutarate aldolase." J Biol Chem 263:11683-11691. Pubmed: 3136164
  • Vlahos, C. J., Dekker, E. E. (1990). "Active-site residues of 2-keto-4-hydroxyglutarate aldolase from Escherichia coli. Bromopyruvate inactivation and labeling of glutamate 45." J Biol Chem 265:20384-20389. Pubmed: 1978721
  • Wymer, N., Buchanan, L. V., Henderson, D., Mehta, N., Botting, C. H., Pocivavsek, L., Fierke, C. A., Toone, E. J., Naismith, J. H. (2001). "Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli." Structure 9:1-9. Pubmed: 11342129