Tryptophan synthase alpha chain (P0A877)

Identification
Name:Tryptophan synthase alpha chain
Synonyms:Not Available
Gene Name:trpA
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Glycine, serine and threonine metabolism ec00260
  • Metabolic pathways eco01100
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
  • Tryptophan metabolism ec00380
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Indole + 1.0L-Serine → 1.0Water + 1.0L-Tryptophan
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Indoleglycerol phosphate + 1.0L-Serine → 1.0D-Glyceraldehyde 3-phosphate + 1.0Water + 1.0L-Tryptophan
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1.0Thumb → 1.0Thumb+1.0Thumb
1.0Indoleglycerol phosphate → 1.0D-Glyceraldehyde 3-phosphate + 1.0Indole
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Serine + 1.0Indole ↔ 1.0L-Tryptophan + 1.0Water
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1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Indoleglycerol phosphate ↔ 1.0Indole + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Serine + 1.0Indoleglycerol phosphate ↔ 1.0L-Tryptophan + 1.0D-Glyceraldehyde 3-phosphate + 1.0Water
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Serine + 1.0Indoleglycerol phosphate + 1.0Indole ↔ 1.0L-Tryptophan + 1.0D-Glyceraldehyde 3-phosphate + 1.0Water
ReactionCard
SMPDB Reactions:
1.0(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate+1.0Thumb → 1.0D-Glyceraldehyde 3-phosphate+1.0Thumb+1.0Thumb
1.0(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + 1.0(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate → 1.0D-Glyceraldehyde 3-phosphate + 1.0Indole + 1.0D-Glyceraldehyde 3-phosphate
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EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Indole + 1.0L-Serine → 1.0Water + 1.0L-Tryptophan
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1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Indoleglycerol phosphate + 1.0L-Serine → 1.0D-Glyceraldehyde 3-phosphate + 1.0Water + 1.0L-Tryptophan
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Indoleglycerol phosphate → 1.0D-Glyceraldehyde 3-phosphate + 1.0Indole
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0glyceraldehyde 3-phosphate+1.0Thumb
1.0L-Serine + 1.0Indoleglycerol phosphate → 1.0L-Tryptophan + 1.0glyceraldehyde 3-phosphate + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24202(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphateMetaboCard
ECMDB01112D-Glyceraldehyde 3-phosphateMetaboCard
ECMDB00738IndoleMetaboCard
ECMDB20160Indoleglycerol phosphateMetaboCard
ECMDB00187L-SerineMetaboCard
ECMDB00929L-TryptophanMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
catalytic activity
hydro-lyase activity
lyase activity
tryptophan synthase activity
Process
cellular amino acid and derivative metabolic process
cellular amino acid derivative metabolic process
cellular biogenic amine metabolic process
cellular metabolic process
indolalkylamine metabolic process
metabolic process
tryptophan metabolic process
Gene Properties
Blattner:b1260
Gene OrientationCounterclockwise
Centisome Percentage:28.33
Left Sequence End1314440
Right Sequence End1315246
Gene Sequence:
>807 bp
ATGGAACGCTACGAATCTCTGTTTGCCCAGTTGAAGGAGCGCAAAGAAGGCGCATTCGTT
CCTTTCGTCACGCTCGGTGATCCGGGCATTGAGCAGTCATTGAAAATTATCGATACGCTA
ATTGAAGCCGGTGCTGACGCGCTGGAGTTAGGTATCCCCTTCTCCGACCCACTGGCGGAT
GGCCCGACGATTCAAAACGCCACTCTGCGCGCCTTTGCGGCAGGTGTGACTCCGGCACAA
TGTTTTGAAATGCTGGCACTGATTCGCCAGAAACACCCGACCATTCCCATTGGCCTGTTG
ATGTATGCCAATCTGGTGTTTAACAAAGGCATTGATGAGTTTTATGCCCAGTGCGAAAAA
GTCGGCGTCGATTCGGTGCTGGTTGCCGATGTGCCAGTTGAAGAGTCCGCGCCCTTCCGC
CAGGCCGCGTTGCGTCATAATGTCGCACCTATCTTCATCTGCCCGCCAAATGCCGATGAC
GACCTGCTGCGCCAGATAGCCTCTTACGGTCGTGGTTACACCTATTTGCTGTCACGAGCA
GGCGTGACCGGCGCAGAAAACCGCGCCGCGTTACCCCTCAATCATCTGGTTGCGAAGCTG
AAAGAGTACAACGCTGCACCTCCATTGCAGGGATTTGGTATTTCCGCCCCGGATCAGGTA
AAAGCAGCGATTGATGCAGGAGCTGCGGGCGCGATTTCTGGTTCGGCCATTGTTAAAATC
ATCGAGCAACATATTAATGAGCCAGAGAAAATGCTGGCGGCACTGAAAGTTTTTGTACAA
CCGATGAAAGCGGCGACGCGCAGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:268
Protein Molecular Weight:28724
Protein Theoretical pI:5
PDB File:1WQ5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Tryptophan synthase alpha chain
MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLAD
GPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEK
VGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRA
GVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKI
IEQHINEPEKMLAALKVFVQPMKAATRS
References
External Links:
ResourceLink
Uniprot ID:P0A877
Uniprot Name:TRPA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742049
PDB ID:1WQ5
Ecogene ID:EG11024
Ecocyc:EG11024
ColiBase:b1260
Kegg Gene:b1260
EchoBASE ID:EB1017
CCDB:TRPA_ECOLI
BacMap:16129221
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Guest, J. R., Drapeau, G. R., Carlton, B. C., Yanofsky, C. (1967). "The amino acid sequence of the A protein (alpha subunit) of the tryptophan synthetase of Escherichia coli." J Biol Chem 242:5442-5446. Pubmed: 4863752
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Milkman, R., Bridges, M. M. (1993). "Molecular evolution of the Escherichia coli chromosome. IV. Sequence comparisons." Genetics 133:455-468. Pubmed: 8095913
  • Nichols, B. P., Yanofsky, C. (1979). "Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison." Proc Natl Acad Sci U S A 76:5244-5248. Pubmed: 388433
  • Tucker, S. D., Murgola, E. J., Pagel, F. T. (1989). "Missense and nonsense suppressors can correct frameshift mutations." Biochimie 71:729-739. Pubmed: 2502189
  • Yanofsky, C., Platt, T., Crawford, I. P., Nichols, B. P., Christie, G. E., Horowitz, H., VanCleemput, M., Wu, A. M. (1981). "The complete nucleotide sequence of the tryptophan operon of Escherichia coli." Nucleic Acids Res 9:6647-6668. Pubmed: 7038627