Polyphosphate kinase (P0A7B1)

Identification
Name:Polyphosphate kinase
Synonyms:
  • ATP-polyphosphate phosphotransferase
  • Polyphosphoric acid kinase
Gene Name:ppk
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can also act in the reverse direction to form ATP in the presence of excess ADP. Can also use GTP instead of ATP; but the efficiency of GTP is 5% that of ATP
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0Adenosine triphosphate + 1.0Polyphosphate ↔ 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Phosphate ↔ 1.0ADP + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Pyrophosphate ↔ 1.0ADP + 1.0Triphosphate
ReactionCard
1.0Thumb+1.0(phosphate)(n) → 1.0Thumb+1.0(phosphate)(n+1)
1.0Adenosine triphosphate + 1.0(phosphate)(n) → 1.0ADP + 1.0(phosphate)(n+1)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB03379TriphosphateMetaboCard
GO Classification:
Component
macromolecular complex
polyphosphate kinase complex
protein complex
Function
catalytic activity
kinase activity
polyphosphate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular metabolic process
metabolic process
phosphate metabolic process
phosphorus metabolic process
polyphosphate biosynthetic process
polyphosphate metabolic process
Gene Properties
Blattner:b2501
Gene OrientationClockwise
Centisome Percentage:56.49
Left Sequence End2621066
Right Sequence End2623132
Gene Sequence:
>2067 bp
ATGGGTCAGGAAAAGCTATACATCGAAAAAGAGCTCAGTTGGTTATCGTTCAATGAACGC
GTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGG
ATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGC
ATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATT
CAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTG
GAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAA
AACTGGCTGCGTCATTATTTTAAGCAGTATCTGCGTCAGCACATTACGCCGATTTTAATC
AATCCTGACACTGACTTAGTGCAGTTCCTGAAAGATGATTACACCTATCTGGCGGTGGAA
ATTATCCGTGGCGATACCATCCGTTACGCGCTGCTGGAGATCCCATCAGATAAAGTGCCG
CGCTTTGTGAATTTACCGCCAGAAGCGCCGCGTCGACGCAAGCCGATGATTCTTCTGGAT
AACATTCTGCGTTACTGCCTTGATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTG
AATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAA
GCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTG
CGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTG
ACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTT
ATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGC
CATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGAT
GTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCT
TCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCA
CGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTT
GAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAA
GCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTG
ATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTT
AACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATC
ACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTT
GATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGC
GAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTT
GTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAAT
CTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATT
CGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAAT
GGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTAT
CGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATC
ATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAAT
CGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTAC
ATCAAATCACTCGAACAACCTGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:688
Protein Molecular Weight:80431
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Polyphosphate kinase
MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNNLDEFYKVRFAELKRR
IIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEMARNQIFLINERQLSVNQQ
NWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVEIIRGDTIRYALLEIPSDKVP
RFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYDALNAYSMKMTRDAEYDLVHEME
ASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVLREKLTISRYDSIVPGGRYHNFKDF
INFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFDAIRERDVLLYYPYHTFEHVLELLRQA
SFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVVELQARFDEEANIHWAKRLTE
AGVHVIFSAPGLKIHAKLFLISRKENGEVVRYAHIGTGNFNEKTARLYTDYSLLTADARI
TNEVRRVFNFIENPYRPVTFDYLMVSPQNSRRLLYEMVDREIANAQQGLPSGITLKLNNL
VDKGLVDRLYAASSSGVPVNLLVRGMCSLIPNLEGISDNIRAISIVDRYLEHDRVYIFEN
GGDKKVYLSSADWMTRNIDYRIEVATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSN
RYVPRGNRRKVRAQLAIYDYIKSLEQPE
References
External Links:
ResourceLink
Uniprot ID:P0A7B1
Uniprot Name:PPK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1805561
Ecogene ID:EG11510
Ecocyc:EG11510
ColiBase:b2501
Kegg Gene:b2501
EchoBASE ID:EB1472
CCDB:PPK_ECOLI
BacMap:16130426
General Reference:
  • Akiyama, M., Crooke, E., Kornberg, A. (1992). "The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." J Biol Chem 267:22556-22561. Pubmed: 1331061
  • Akiyama, M., Crooke, E., Kornberg, A. (1993). "An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon." J Biol Chem 268:633-639. Pubmed: 8380170
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kumble, K. D., Ahn, K., Kornberg, A. (1996). "Phosphohistidyl active sites in polyphosphate kinase of Escherichia coli." Proc Natl Acad Sci U S A 93:14391-14395. Pubmed: 8962061
  • Smith, J. M., Daum, H. A. 3rd (1987). "Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12." J Biol Chem 262:10565-10569. Pubmed: 3301838
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837