Phosphoglycerate kinase (P0A799)

Identification
Name:Phosphoglycerate kinase
Synonyms:Not Available
Gene Name:pgk
Enzyme Class:
Biological Properties
General Function:Involved in phosphoglycerate kinase activity
Specific Function:ATP + 3-phospho-D-glycerate = ADP + 3-phospho- D-glyceroyl phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
  • Carbon fixation in photosynthetic organisms ec00710
  • Glycolysis / Gluconeogenesis ec00010
  • Metabolic pathways eco01100
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.03-Phospho-D-glycerate ↔ 1.0ADP + 1.0Glyceric acid 1,3-biphosphate + 1.03-phospho-D-glyceroyl phosphate
ReactionCard
SMPDB Reactions:
1.0Glyceric acid 1,3-biphosphate+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.03-Phosphoglyceric acid+1.0Thumb
1.0Glyceric acid 1,3-biphosphate + 1.0Adenosine diphosphate + 1.0Glyceric acid 1,3-biphosphate + 1.0ADP → 1.0Adenosine triphosphate + 1.03-Phosphoglyceric acid + 1.03-Phosphoglycerate
ReactionCard
1.03-Phosphoglyceric acid+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Glyceric acid 1,3-biphosphate+1.0Thumb+1.0Thumb
1.03-Phosphoglyceric acid + 1.0Adenosine triphosphate + 1.03-Phosphoglycerate → 1.0Adenosine diphosphate + 1.0Glyceric acid 1,3-biphosphate + 1.0ADP + 1.0Glyceric acid 1,3-biphosphate
ReactionCard
1.03-Phosphoglyceric acid+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb
1.03-Phosphoglyceric acid + 1.0Adenosine triphosphate + 1.03-Phosphoglycerate → 1.03-phospho-D-glyceroyl phosphate + 1.0Adenosine diphosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.03-Phosphoglycerate + 1.0Adenosine triphosphate ↔ 1.0Glyceric acid 1,3-biphosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.03-Phospho-D-glycerate → 1.0ADP + 1.03-phospho-D-glyceroyl phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB216453-Phospho-D-glycerateMetaboCard
ECMDB231413-phospho-D-glyceroyl phosphateMetaboCard
ECMDB041313-PhosphoglycerateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01270Glyceric acid 1,3-biphosphateMetaboCard
GO Classification:
Function
catalytic activity
kinase activity
phosphoglycerate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
alcohol metabolic process
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b2926
Gene OrientationCounterclockwise
Centisome Percentage:66.16
Left Sequence End3069481
Right Sequence End3070644
Gene Sequence:
>1164 bp
ATGTCTGTAATTAAGATGACCGATCTGGATCTTGCTGGGAAACGTGTATTTATCCGTGCG
GATCTGAACGTACCAGTAAAAGACGGGAAAGTAACCAGCGACGCGCGTATCCGTGCTTCT
CTGCCGACCATCGAACTGGCCCTGAAACAAGGCGCAAAAGTGATGGTAACTTCCCACCTG
GGTCGTCCTACCGAAGGCGAGTACAACGAAGAATTCTCTCTGCTGCCGGTTGTTAACTAC
CTGAAAGACAAACTGTCTAACCCGGTTCGTCTGGTTAAAGATTACCTCGACGGCGTTGAC
GTTGCTGAAGGTGAACTGGTTGTTCTGGAAAACGTTCGCTTCAACAAAGGCGAGAAGAAA
GACGACGAAACCCTGTCCAAAAAATACGCTGCACTGTGTGACGTGTTCGTAATGGACGCA
TTCGGTACTGCTCACCGCGCGCAGGCTTCTACTCACGGTATCGGTAAATTCGCTGACGTT
GCGTGCGCAGGCCCGCTGCTGGCAGCTGAACTGGACGCGCTGGGTAAAGCACTGAAAGAA
CCTGCTCGCCCGATGGTGGCTATCGTTGGTGGTTCTAAAGTATCTACCAAACTGACCGTT
CTGGACTCCCTGTCTAAAATCGCTGACCAGCTGATTGTTGGTGGTGGTATCGCTAACACC
TTTATCGCGGCACAAGGCCACGATGTGGGTAAATCCCTGTACGAAGCTGACCTGGTTGAC
GAAGCTAAACGTCTGCTGACCACCTGCAACATCCCGGTTCCGTCTGATGTTCGCGTAGCA
ACCGAGTTCTCTGAAACTGCACCGGCTACCCTGAAATCTGTTAACGATGTGAAAGCTGAC
GAGCAGATCCTGGATATCGGTGATGCTTCCGCTCAGGAACTGGCTGAAATCCTGAAGAAT
GCGAAAACCATTCTGTGGAACGGTCCGGTTGGCGTGTTCGAATTCCCGAACTTCCGCAAA
GGTACTGAAATCGTGGCTAACGCTATCGCAGACAGCGAAGCGTTCTCCATCGCTGGCGGC
GGCGACACTCTGGCAGCAATCGACCTGTTCGGCATTGCTGACAAAATCTCCTACATCTCC
ACTGGCGGCGGCGCATTCCTCGAATTCGTGGAAGGTAAAGTACTGCCTGCAGTAGCGATG
CTCGAAGAGCGCGCTAAGAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:387
Protein Molecular Weight:41118
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphoglycerate kinase
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHL
GRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKK
DDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVACAGPLLAAELDALGKALKE
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
EAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKN
AKTILWNGPVGVFEFPNFRKGTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYIS
TGGGAFLEFVEGKVLPAVAMLEERAKK
References
External Links:
ResourceLink
Uniprot ID:P0A799
Uniprot Name:PGK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675737
Ecogene ID:EG10703
Ecocyc:EG10703
ColiBase:b2926
Kegg Gene:b2926
EchoBASE ID:EB0697
CCDB:PGK_ECOLI
BacMap:16130827
General Reference:
  • Alefounder, P. R., Perham, R. N. (1989). "Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli." Mol Microbiol 3:723-732. Pubmed: 2546007
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841
  • Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842