Glutamate--cysteine ligase (P0A6W9)

Identification
Name:Glutamate--cysteine ligase
Synonyms:
  • Gamma-ECS
  • GCS
  • Gamma-glutamylcysteine synthetase
Gene Name:gshA
Enzyme Class:
Biological Properties
General Function:Involved in glutamate-cysteine ligase activity
Specific Function:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Cysteine + 1.0L-Glutamate ↔ 1.0ADP + 1.0gamma-Glutamylcysteine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Glutamate + 1.0L-Cysteine ↔ 1.0ADP + 1.0Phosphate + 1.0gamma-Glutamylcysteine
ReactionCard
SMPDB Reactions:
1.0L-Glutamic acid+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Glutamic acid + 1.0Adenosine triphosphate + 1.0L-Cysteine + 1.0L-Glutamate → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0gamma-Glutamylcysteine + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Cysteine + 1.0L-Glutamate ↔ 1.0ADP + 1.0gamma-Glutamylcysteine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Cysteine + 1.0L-Glutamate + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0gamma-Glutamylcysteine + 1.0Phosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Glutamate + 1.0L-Cysteine → 1.0ADP + 1.0Inorganic phosphate + 1.0gamma-Glutamylcysteine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01049gamma-GlutamylcysteineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00574L-CysteineMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Function
acid-amino acid ligase activity
catalytic activity
glutamate-cysteine ligase activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
Process
cellular metabolic process
glutathione biosynthetic process
glutathione metabolic process
metabolic process
peptide metabolic process
Gene Properties
Blattner:b2688
Gene OrientationCounterclockwise
Centisome Percentage:60.63
Left Sequence End2812905
Right Sequence End2814461
Gene Sequence:
>1557 bp
ATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGC
GTAATGATTGCGATACTGGTGAGTTGCCTTCAGTTTTTAGTGGCCTGGCATAAGCACGAA
GTCAAATACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGAC
CTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAC
CCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAA
GATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCAATGAATTG
ATTCCGGCGCTCGACATTAATAAAAACGTCGATATGGCGATCTTACCCGGCACGCCGATG
GTGCCGAACAAACCCGCAATCGTCATCTGGTATCGCAACCCTTTGCTGAAAAATAGCGGC
GTCTTTGCCGCTCTGAATCTCAACCTGACGCCTTCACTCTTTTATAGTTCACGGCAGGAA
GATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGT
TTGATGAACGTTAACGAATTAACCGACATGCCAGTCCGTGAAACTAAAATTGCGGGCATT
CCTCTGACCGTTCGGCTTTATGCAGATGACTGGACATGGAACGATGTGTGGTACGCATTT
TTACTGGGCGGCATGAGTGGAACTGTCGTTGGCCTGCTCTGCTATTACCTGATGAGCGTA
CGTATGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCG
TATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGC
TGGCGGCATCCTGTCGCGGGAGAAATTCCCCCGGATGCCTTCATTAACTTTGCCGAATCG
CAAAAGATGATTGTGCCGCTGACTCAGCACCTGTTTGAGTTAATTGCCCGCGATGCCGCA
GAATTAGAAAAAGTGCTGCCGGTAGGCGTCAAATTTGGTATTAACATTGCGCCGGACCAT
CTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTGCCCGCACAC
CATTTCCAGATTGTGCTGGAAATTACCGAGCGCGATATGTTGAAAGAGCAAGAAGCCACA
CAACTCTTCGCCTGGCTGCACTCGGTCGGCGTAGAAATTGCTATTGATGACTTCGGCACC
GGGCACAGCGCGCTTATCTATCTTGAGCGTTTTACGCTCGATTATCTGAAAATTGACCGT
GGATTTATCAACGCCATCGGTACGGAAACGATCACTTCCCCCGTACTTGACGCGGTGCTG
ACGCTGGCGAAACGCCTCAATATGCTGACGGTTGCTGAAGGGGTCGAAACGCCGGAACAG
GCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCG
TTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGTGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:518
Protein Molecular Weight:58269
Protein Theoretical pI:5
PDB File:1VA6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glutamate--cysteine ligase
MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGHPEALGSALTHKWITTD
FAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPCYIAEGQDIELAQYG
TSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGDISGADAKEKISAGY
FRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPFEKTECGMYYLPYATSLRLSDLG
YTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIENELY
APIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQVRFLDLFMVWCALA
DAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLKRVAQTL
DSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKAFAEAYRNLLREEPL
EILREEDFVAEREASERRQQEMEAADTEPFAVWLEKHA
References
External Links:
ResourceLink
Uniprot ID:P0A6W9
Uniprot Name:GSH1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736841
PDB ID:1VA6
Ecogene ID:EG10418
Ecocyc:EG10418
ColiBase:b2688
Kegg Gene:b2688
EchoBASE ID:EB0413
CCDB:GSH1_ECOLI
BacMap:16130600
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Watanabe, K., Yamano, Y., Murata, K., Kimura, A. (1986). "The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli." Nucleic Acids Res 14:4393-4400. Pubmed: 2872655
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837