Glycerophosphoryl diester phosphodiesterase (P09394)

Identification
Name:Glycerophosphoryl diester phosphodiesterase
Synonyms:
  • Glycerophosphodiester phosphodiesterase
Gene Name:glpQ
Enzyme Class:
Biological Properties
General Function:Involved in glycerophosphodiester phosphodiesterase activity
Specific Function:Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols
Cellular Location:Periplasm
SMPDB Pathways:
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glycerophosphocholine + 1.0Water ↔ 1.0Choline + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glycerylphosphorylethanolamine + 1.0Water ↔ 1.0Ethanolamine + 1.0Glycerol 3-phosphate
ReactionCard
1.0Glycerophosphodiester+1.0Thumb ↔ 1.0Alcohol+1.0Thumb
1.0Glycerophosphodiester + 1.0Water ↔ 1.0Alcohol + 1.0Glycerol 3-phosphate
ReactionCard
SMPDB Reactions:
1.0a glycerophosphodiester+1.0Thumb → 1.0Thumb+1.0Alcohol+1.0Thumb
1.0a glycerophosphodiester + 1.0Water → 1.0Hydrogen ion + 1.0Alcohol + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0sn-glycero-3-phosphocholine + 1.0Water → 1.0Hydrogen ion + 1.0Benzyl alcohol + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0sn-glycero-3-phosphoethanolamine + 1.0Water → 1.0Benzyl alcohol + 1.0Hydrogen ion + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerophosphoglycerol + 1.0Water → 1.0Benzyl alcohol + 1.0Hydrogen ion + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0 glycerophosphoserine + 1.0Water → 1.0Hydrogen ion + 1.0Benzyl alcohol + 1.0Glycerol 3-phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerophosphocholine + 1.0Water → 1.0Choline + 1.0Glycerol 3-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerylphosphorylethanolamine + 1.0Water → 1.0Ethanolamine + 1.0Glycerol 3-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerophosphoglycerol + 1.0Water → 1.0Glycerol + 1.0Glycerol 3-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerophosphoserine + 1.0Water → 1.0Glycerol 3-phosphate + 1.0Hydrogen ion + 1.0L-Serine
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Sn-Glycero-3-phospho-1-inositol + 1.0Water → 1.0Glycerol 3-phosphate + 1.0Hydrogen ion + 1.0Inositol
ReactionCard
1.0A glycerophosphodiester+1.0Thumb → 1.0an alcohol+1.0Thumb
1.0A glycerophosphodiester + 1.0Water → 1.0an alcohol + 1.0Glycerol 3-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24250 glycerophosphoserineMetaboCard
ECMDB24002Benzyl alcoholMetaboCard
ECMDB00097CholineMetaboCard
ECMDB00149EthanolamineMetaboCard
ECMDB00131GlycerolMetaboCard
ECMDB00126Glycerol 3-phosphateMetaboCard
ECMDB00086GlycerophosphocholineMetaboCard
ECMDB21223GlycerophosphoglycerolMetaboCard
ECMDB21224GlycerophosphoserineMetaboCard
ECMDB00114GlycerylphosphorylethanolamineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00160InositolMetaboCard
ECMDB00187L-SerineMetaboCard
ECMDB21357Sn-Glycero-3-phospho-1-inositolMetaboCard
ECMDB24248sn-glycero-3-phosphocholineMetaboCard
ECMDB24249sn-glycero-3-phosphoethanolamineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
glycerophosphodiester phosphodiesterase activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphoric diester hydrolase activity
phosphoric ester hydrolase activity
Process
alcohol metabolic process
alditol metabolic process
glycerol metabolic process
lipid metabolic process
metabolic process
polyol metabolic process
primary metabolic process
small molecule metabolic process
Gene Properties
Blattner:b2239
Gene OrientationCounterclockwise
Centisome Percentage:50.61
Left Sequence End2347957
Right Sequence End2349033
Gene Sequence:
>1077 bp
ATGAAATTGACGCTGAAAAACCTTAGCATGGCGATCATGATGAGCACTATAGTCATGGGA
AGCAGTGCAATGGCGGCGGACAGCAACGAAAAAATAGTCATCGCCCATCGCGGTGCCAGT
GGATATTTGCCGGAGCATACGCTGCCAGCAAAAGCGATGGCGTATGCGCAGGGAGCGGAT
TATCTGGAACAGGATTTGGTGATGACCAAAGACGACAATCTGGTTGTTCTGCATGACCAT
TACCTCGATCGTGTTACTGATGTTGCCGATCGTTTCCCGGATCGGGCGCGCAAAGACGGT
CGTTACTACGCGATAGATTTCACGCTGGATGAAATTAAGTCGTTGAAATTTACCGAAGGT
TTCGATATTGAAAACGGTAAAAAAGTGCAGACTTATCCGGGGCGTTTCCCAATGGGTAAG
TCCGACTTCCGGGTGCACACCTTTGAAGAAGAGATTGAATTTGTTCAGGGGTTAAATCAC
TCTACCGGGAAAAATATCGGTATTTATCCAGAAATCAAAGCGCCGTGGTTCCATCATCAG
GAAGGGAAGGATATTGCGGCAAAAACGCTGGAAGTGCTGAAGAAATATGGTTACACCGGT
AAAGACGATAAAGTTTATTTGCAATGTTTTGATGCTGATGAGCTGAAGCGTATTAAGAAT
GAGCTGGAACCCAAAATGGGCATGGAGCTCAATCTGGTACAGCTGATTGCCTATACCGAC
TGGAATGAAACGCAGCAGAAACAGCCGGATGGAAGCTGGGTTAATTACAACTACGACTGG
ATGTTTAAGCCGGGTGCCATGAAACAGGTGGCGGAATATGCAGATGGTATTGGTCCGGAT
TACCATATGTTGATTGAGGAGACATCGCAGCCGGGTAATATCAAACTCACTGGCATGGTG
CAAGATGCTCAGCAGAATAAACTGGTAGTGCATCCTTATACCGTGCGGTCAGATAAACTG
CCTGAATACACTCCTGATGTGAATCAGTTATATGATGCTCTGTATAACAAAGCGGGTGTA
AATGGGCTGTTTACTGATTTCCCTGATAAGGCAGTAAAATTTCTTAATAAAGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:358
Protein Molecular Weight:40843
Protein Theoretical pI:5
PDB File:1YDY
Signaling Regions:
  • 1-25
Transmembrane Regions:
  • None
Protein Sequence:
>Glycerophosphoryl diester phosphodiesterase
MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLPEHTLPAKAMAYAQGAD
YLEQDLVMTKDDNLVVLHDHYLDRVTDVADRFPDRARKDGRYYAIDFTLDEIKSLKFTEG
FDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNHSTGKNIGIYPEIKAPWFHHQ
EGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRIKNELEPKMGMELNLVQLIAYTD
WNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADGIGPDYHMLIEETSQPGNIKLTGMV
QDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDALYNKAGVNGLFTDFPDKAVKFLNKE
References
External Links:
ResourceLink
Uniprot ID:P09394
Uniprot Name:GLPQ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1799586
PDB ID:1YDY
Ecogene ID:EG10399
Ecocyc:EG10399
ColiBase:b2239
Kegg Gene:b2239
EchoBASE ID:EB0394
CCDB:GLPQ_ECOLI
BacMap:16130174
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Eiglmeier, K., Boos, W., Cole, S. T. (1987). "Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system." Mol Microbiol 1:251-258. Pubmed: 3329281
  • Gonzalez-Gil, G., Bringmann, P., Kahmann, R. (1996). "FIS is a regulator of metabolism in Escherichia coli." Mol Microbiol 22:21-29. Pubmed: 8899705
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Larson, T. J., van Loo-Bhattacharya, A. T. (1988). "Purification and characterization of glpQ-encoded glycerophosphodiester phosphodiesterase from Escherichia coli K-12." Arch Biochem Biophys 260:577-584. Pubmed: 2829735
  • Tommassen, J., Eiglmeier, K., Cole, S. T., Overduin, P., Larson, T. J., Boos, W. (1991). "Characterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli." Mol Gen Genet 226:321-327. Pubmed: 1851953
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837