Homoserine O-succinyltransferase (P07623)

Identification
Name:Homoserine O-succinyltransferase
Synonyms:
  • Homoserine O-transsuccinylase
  • HTS
Gene Name:metA
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring acyl groups
Specific Function:Succinyl-CoA + L-homoserine = CoA + O- succinyl-L-homoserine
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Homoserine + 1.0Succinyl-CoA ↔ 1.0Coenzyme A + 1.0O-Succinyl-L-homoserine
ReactionCard
SMPDB Reactions:
1.0L-Homoserine+1.0Succinyl-CoA+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Homoserine + 1.0Succinyl-CoA + 1.0L-Homoserine + 1.0Succinyl-CoA → 1.0Coenzyme A + 1.0O-Succinyl-L-homoserine
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Homoserine + 1.0Succinyl-CoA ↔ 1.0Coenzyme A + 1.0O-Succinyl-L-homoserine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01423Coenzyme AMetaboCard
ECMDB02251L-HomoserineMetaboCard
ECMDB01418O-Succinyl-L-homoserineMetaboCard
ECMDB01022Succinyl-CoAMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acyltransferase activity
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
homoserine metabolic process
L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine
L-methionine biosynthetic process from L-homoserine via cystathionine
metabolic process
serine family amino acid metabolic process
Gene Properties
Blattner:b4013
Gene OrientationClockwise
Centisome Percentage:90.79
Left Sequence End4212303
Right Sequence End4213232
Gene Sequence:
>930 bp
ATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCT
GTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATC
GACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTC
TCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGAT
CGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTC
AGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGG
AAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTG
CCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTG
AAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTC
GCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTC
TGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCG
GCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGG
GACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAG
CTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTC
TCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAA
ACCCTGCTGCAACAGCTCAAGCTTTGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:309
Protein Molecular Weight:35727
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Homoserine O-succinyltransferase
MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLMPKKIETENQFLRLLSN
SPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQDQNFDGLIVTGAPLGLVEFNDVAY
WPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTRTEKLSGVYEHHILHPHALLT
RGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGDAYLFASKDKRIAFVTGHPEYDA
QTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRASWRSHGNLLFTNWLNYYVYQITPYD
LRHMNPTLD
References
External Links:
ResourceLink
Uniprot ID:P07623
Uniprot Name:META_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674412
Ecogene ID:EG10581
Ecocyc:EG10581
ColiBase:b4013
Kegg Gene:b4013
EchoBASE ID:EB0576
CCDB:META_ECOLI
BacMap:16131839
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Born, T. L., Blanchard, J. S. (1999). "Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase." Biochemistry 38:14416-14423. Pubmed: 10572016
  • Byrne, C., Stokes, H. W., Ward, K. A. (1988). "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Nucleic Acids Res 16:9342. Pubmed: 3050899
  • Duclos, B., Cortay, J. C., Bleicher, F., Ron, E. Z., Richaud, C., Saint Girons, I., Cozzone, A. J. (1989). "Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli." Nucleic Acids Res 17:2856. Pubmed: 2654885
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Michaeli, S., Mevarech, M., Ron, E. Z. (1984). "Regulatory region of the metA gene of Escherichia coli K-12." J Bacteriol 160:1158-1162. Pubmed: 6094503