NAD(P) transhydrogenase subunit alpha (P07001)

Identification
Name:NAD(P) transhydrogenase subunit alpha
Synonyms:
  • Nicotinamide nucleotide transhydrogenase subunit alpha
  • Pyridine nucleotide transhydrogenase subunit alpha
Gene Name:pntA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • NAD phosphorylation and dephosphorylation PW002081
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NAD + 1.0NADPH ↔ 1.0NADH + 1.0NADP
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADH + 1.0NADP → 1.0NADPH + 1.0NAD
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NAD + 1.0NADPH ↔ 1.0NADH + 1.0NADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+2.0Thumb → 2.0Thumb+1.0Thumb+1.0Thumb
1.0NADH + 1.0NADP + 2.0Hydrogen ion → 2.0Hydrogen ion + 1.0NAD + 1.0NADPH
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0NADPH + 1.0NAD → 1.0NADP + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
binding
catalytic activity
NAD(P)+ transhydrogenase (AB-specific) activity
NAD(P)+ transhydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
oxidoreductase activity, acting on NADH or NADPH, NAD or NADP as acceptor
Process
establishment of localization
hydrogen transport
metabolic process
oxidation reduction
proton transport
transport
Gene Properties
Blattner:b1603
Gene OrientationCounterclockwise
Centisome Percentage:36.09
Left Sequence End1674395
Right Sequence End1675927
Gene Sequence:
>1533 bp
ATGCGAATTGGCATACCAAGAGAACGGTTAACCAATGAAACCCGTGTTGCAGCAACGCCA
AAAACAGTGGAACAGCTGCTGAAACTGGGTTTTACCGTCGCGGTAGAGAGCGGCGCGGGT
CAACTGGCAAGTTTTGACGATAAAGCGTTTGTGCAAGCGGGCGCTGAAATTGTAGAAGGG
AATAGCGTCTGGCAGTCAGAGATCATTCTGAAGGTCAATGCGCCGTTAGATGATGAAATT
GCGTTACTGAATCCTGGGACAACGCTGGTGAGTTTTATCTGGCCTGCGCAGAATCCGGAA
TTAATGCAAAAACTTGCGGAACGTAACGTGACCGTGATGGCGATGGACTCTGTGCCGCGT
ATCTCACGCGCACAATCGCTGGACGCACTAAGCTCGATGGCGAACATCGCCGGTTATCGC
GCCATTGTTGAAGCGGCACATGAATTTGGGCGCTTCTTTACCGGGCAAATTACTGCGGCC
GGGAAAGTGCCACCGGCAAAAGTGATGGTGATTGGTGCGGGTGTTGCAGGTCTGGCCGCC
ATTGGCGCAGCAAACAGTCTCGGCGCGATTGTGCGTGCATTCGACACCCGCCCGGAAGTG
AAAGAACAAGTTCAAAGTATGGGCGCGGAATTCCTCGAGCTGGATTTTAAAGAGGAAGCT
GGCAGCGGCGATGGCTATGCCAAAGTGATGTCGGACGCGTTCATCAAAGCGGAAATGGAA
CTCTTTGCCGCCCAGGCAAAAGAGGTCGATATCATTGTCACCACCGCGCTTATTCCAGGC
AAACCAGCGCCGAAGCTAATTACCCGTGAAATGGTTGACTCCATGAAGGCGGGCAGTGTG
ATTGTCGACCTGGCAGCCCAAAACGGCGGCAACTGTGAATACACCGTGCCGGGTGAAATC
TTCACTACGGAAAATGGTGTCAAAGTGATTGGTTATACCGATCTTCCGGGCCGTCTGCCG
ACGCAATCCTCACAGCTTTACGGCACAAACCTCGTTAATCTGCTGAAACTGTTGTGCAAA
GAGAAAGACGGCAATATCACTGTTGATTTTGATGATGTGGTGATTCGCGGCGTGACCGTG
ATCCGTGCGGGCGAAATTACCTGGCCGGCACCGCCGATTCAGGTATCAGCTCAGCCGCAG
GCGGCACAAAAAGCGGCACCGGAAGTGAAAACTGAGGAAAAATGTACCTGCTCACCGTGG
CGTAAATACGCGTTGATGGCGCTGGCAATCATTCTTTTTGGCTGGATGGCAAGCGTTGCG
CCGAAAGAATTCCTTGGGCACTTCACCGTTTTCGCGCTGGCCTGCGTTGTCGGTTATTAC
GTGGTGTGGAATGTATCGCACGCGCTGCATACACCGTTGATGTCGGTCACCAACGCGATT
TCAGGGATTATTGTTGTCGGAGCACTGTTGCAGATTGGCCAGGGCGGCTGGGTTAGCTTC
CTTAGTTTTATCGCGGTGCTTATAGCCAGCATTAATATTTTCGGTGGCTTCACCGTGACT
CAGCGCATGCTGAAAATGTTCCGCAAAAATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:510
Protein Molecular Weight:54623
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • 402-422
  • 423-443
  • 453-473
  • 477-497
Protein Sequence:
>NAD(P) transhydrogenase subunit alpha
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEG
NSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPR
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
LFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEI
FTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTV
IRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPWRKYALMALAIILFGWMASVA
PKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
References
External Links:
ResourceLink
Uniprot ID:P07001
Uniprot Name:PNTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742643
Ecogene ID:EG10744
Ecocyc:EG10744
ColiBase:b1603
Kegg Gene:b1603
EchoBASE ID:EB0737
CCDB:PNTA_ECOLI
BacMap:16129561
General Reference:
  • Ahmad, S., Glavas, N. A., Bragg, P. D. (1992). "A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change." Eur J Biochem 207:733-739. Pubmed: 1633824
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Clarke, D. M., Loo, T. W., Gillam, S., Bragg, P. D. (1986). "Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli." Eur J Biochem 158:647-653. Pubmed: 3525165
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Johansson, T., Oswald, C., Pedersen, A., Tornroth, S., Okvist, M., Karlsson, B. G., Rydstrom, J., Krengel, U. (2005). "X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli." J Mol Biol 352:299-312. Pubmed: 16083909
  • Tong, R. C., Glavas, N. A., Bragg, P. D. (1991). "Topological analysis of the pyridine nucleotide transhydrogenase of Escherichia coli using proteolytic enzymes." Biochim Biophys Acta 1080:19-28. Pubmed: 1932078