Identification
Name:Aromatic-amino-acid aminotransferase
Synonyms:
  • ARAT
  • AROAT
Gene Name:tyrB
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity
Specific Function:An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.04-hydroxyphenylpyruvate+1.0L-Glutamic acid+1.0Thumb1.0Thumb+1.0L-Tyrosine+1.0Thumb
1.04-hydroxyphenylpyruvate + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Tyrosine + 1.0L-Tyrosine
ReactionCard
1.0Thumb+1.0L-Glutamic acid+1.0Thumb1.0Thumb+1.0Thumb
1.0Ketoleucine + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Leucine
ReactionCard
1.0L-Phenylalanine+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0An aromatic amino acid+1.0Thumb1.0an aromatic oxo acid+1.0Thumb
1.0An aromatic amino acid + 1.0Oxoglutaric acid → 1.0an aromatic oxo acid + 1.0L-Glutamate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB015532-Oxo-4-methylthiobutanoic acidMetaboCard
ECMDB007074-Hydroxyphenylpyruvic acidMetaboCard
ECMDB02812alpha-KetoglutarateMetaboCard
ECMDB00695KetoleucineMetaboCard
ECMDB00191L-Aspartic acidMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB00687L-LeucineMetaboCard
ECMDB00696L-MethionineMetaboCard
ECMDB00159L-PhenylalanineMetaboCard
ECMDB00158L-TyrosineMetaboCard
ECMDB00223Oxalacetic acidMetaboCard
ECMDB04123Oxoglutaric acidMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB00205Phenylpyruvic acidMetaboCard
ECMDB12283PrephenateMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
pyridoxal phosphate binding
transaminase activity
transferase activity
transferase activity, transferring nitrogenous groups
Process
biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b4054
Gene OrientationClockwise
Centisome Percentage:91.93
Left Sequence End4265137
Right Sequence End4266330
Gene Sequence:
>1194 bp
ATGAGAGCCTGCATTAATAATCAACAGATTCGCCACCATAACAAATGCGTGATTCTGGAA
CTGCTGTACCGGCAAAAGCGCGCCAATAAATCAACGCTGGCCCGGCTGGCGCAAATTTCG
ATTCCGGCAGTCAGTAATATTTTGCAGGAACTGGAAAGCGAAAAACGGGTGGTGAATATT
GACGATGAAAGCCAGACGCGCGGGCATAGTAGCGGTACATGGCTGATTGCGCCGGAAGGT
GACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCT
TGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCG
CTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACC
ATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAACC
ATGCCGCAAGCGCCGTGGACAACGCCGGTGGAGGTGAAATATCTGCTGGAAGAGAAGCTC
GGCATTCGGGTGATGGTCGATAATGACTGCGTGATGCTGGCGCTGGCGGAGAAATGGCAA
AATAATTCGCAGGAACGGGATTTCTGCGTGATCAACGTTGATTACGGCATTGGCTCGTCG
TTCGTGATTAACGAGCAAATTTATCGCGGCAGTTTGTATGGTAGCGGACAGATTGGTCAC
ACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACT
GTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTT
AGTACTCAACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGT
GGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTG
TATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAGTTGTGCC
TTTGGTGAGAACTGGCTTAATACTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGC
GACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAA
CAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:397
Protein Molecular Weight:43537
Protein Theoretical pI:5
PDB File:3TAT
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Aromatic-amino-acid aminotransferase
MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIPQLQAVAEAEARLNAQP
HGASLYLPMEGLNCYRHAIAPLLFGADHPVLKQQRVATIQTLGGSGALKVGADFLKRYFP
ESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRFNDLLATLKTLPARSIVLLHP
CCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGFGAGMEEDAYAIRAIASAGLPAL
VSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQLKATVRRNYSSPPNFGAQVVAAVLN
DEALKASWLAEVEEMRTRILAMRQELVKVLSTEMPERNFDYLLNQRGMFSYTGLSAAQVD
RLREEFGVYLIASGRMCVAGLNTANVQRVAKAFAAVM
References
External Links:
ResourceLink
Uniprot ID:P04693
Uniprot Name:TYRB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675445
PDB ID:3TAT
Ecogene ID:EG11040
Ecocyc:EG11040
ColiBase:b4054
Kegg Gene:b4054
EchoBASE ID:EB1033
CCDB:TYRB_ECOLI
BacMap:16131880
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fotheringham, I. G., Dacey, S. A., Taylor, P. P., Smith, T. J., Hunter, M. G., Finlay, M. E., Primrose, S. B., Parker, D. M., Edwards, R. M. (1986). "The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes." Biochem J 234:593-604. Pubmed: 3521591
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ko, T. P., Wu, S. P., Yang, W. Z., Tsai, H., Yuan, H. S. (1999). "Crystallization and preliminary crystallographic analysis of the Escherichia coli tyrosine aminotransferase." Acta Crystallogr D Biol Crystallogr 55:1474-1477. Pubmed: 10417420
  • Kuramitsu, S., Inoue, K., Ogawa, T., Ogawa, H., Kagamiyama, H. (1985). "Aromatic amino acid aminotransferase of Escherichia coli: nucleotide sequence of the tyrB gene." Biochem Biophys Res Commun 133:134-139. Pubmed: 3907634
  • Yang, J., Pittard, J. (1987). "Molecular analysis of the regulatory region of the Escherichia coli K-12 tyrB gene." J Bacteriol 169:4710-4715. Pubmed: 3308851