ECMDB

Identification

Name:

Aromatic-amino-acid aminotransferase

Synonyms:

ARAT
AROAT

Gene Name:

tyrB

Enzyme Class:

2.6.1.57

Biological Properties

General Function:

Involved in transferase activity

Specific Function:

An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate

Cellular Location:

Cytoplasm

SMPDB Pathways:

Leucine Biosynthesis PW000811
Lysine biosynthesis PW000771
Phenylalanine metabolism PW000921
Secondary Metabolite: Leucine biosynthesis PW000980
tyrosine biosynthesis PW000806

KEGG Pathways:

Cysteine and methionine metabolism ec00270
Isoquinoline alkaloid biosynthesis ec00950
Lysine biosynthesis ec00300
Metabolic pathways eco01100
Novobiocin biosynthesis ec00401
Phenylalanine metabolism ec00360
Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
Tropane, piperidine and pyridine alkaloid biosynthesis ec00960
Tyrosine metabolism ec00350

KEGG Reactions:

1.0

↔

1.0

1.0alpha-Ketoglutarate + 1.0L-Phenylalanine ↔ 1.0L-Glutamate + 1.0Phenylpyruvic acid
ReactionCard

1.0

↔

1.0

1.0alpha-Ketoglutarate + 1.0L-Tyrosine ↔ 1.04-Hydroxyphenylpyruvic acid + 1.0L-Glutamate
ReactionCard

1.0

↔

1.0

1.02-Oxo-4-methylthiobutanoic acid + 1.0L-Glutamate ↔ 1.0L-Methionine + 1.0alpha-Ketoglutarate
ReactionCard

1.0

↔

1.0

1.0Aromatic amino acid + 1.0alpha-Ketoglutarate ↔ 1.0Aromatic oxo acid + 1.0L-Glutamate
ReactionCard

SMPDB Reactions:

1.04-hydroxyphenylpyruvate

1.0L-Glutamic acid

1.0

→

1.0

1.0L-Tyrosine

1.0

1.04-hydroxyphenylpyruvate + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Tyrosine + 1.0L-Tyrosine
ReactionCard

1.0

1.0L-Glutamic acid

1.0

→

1.0

1.0Ketoleucine + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Leucine
ReactionCard

1.0L-Phenylalanine

1.0

↔

1.0

1.0L-Phenylalanine + 1.0Oxygen + 1.0L-Phenylalanine ↔ 1.0Oxoglutaric acid + 1.0Phenylpyruvic acid
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Ketoleucine + 1.0L-Glutamate → 1.0alpha-Ketoglutarate + 1.0L-Leucine
ReactionCard

1.0An aromatic amino acid

1.0

→

1.0an aromatic oxo acid

1.0

1.0An aromatic amino acid + 1.0Oxoglutaric acid → 1.0an aromatic oxo acid + 1.0L-Glutamate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01553	2-Oxo-4-methylthiobutanoic acid	MetaboCard
ECMDB00707	4-Hydroxyphenylpyruvic acid	MetaboCard
ECMDB02812	alpha-Ketoglutarate	MetaboCard
ECMDB00695	Ketoleucine	MetaboCard
ECMDB00191	L-Aspartic acid	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB00687	L-Leucine	MetaboCard
ECMDB00696	L-Methionine	MetaboCard
ECMDB00159	L-Phenylalanine	MetaboCard
ECMDB00158	L-Tyrosine	MetaboCard
ECMDB00223	Oxalacetic acid	MetaboCard
ECMDB04123	Oxoglutaric acid	MetaboCard
ECMDB04124	Oxygen	MetaboCard
ECMDB00205	Phenylpyruvic acid	MetaboCard
ECMDB12283	Prephenate	MetaboCard

GO Classification:

Function
binding
catalytic activity
cofactor binding
pyridoxal phosphate binding
transaminase activity
transferase activity
transferase activity, transferring nitrogenous groups
Process
biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process

Gene Properties

Blattner:

b4054

Gene Orientation

Clockwise

Centisome Percentage:

91.93

Left Sequence End

4265137

Right Sequence End

4266330

Gene Sequence:

>1194 bp
ATGAGAGCCTGCATTAATAATCAACAGATTCGCCACCATAACAAATGCGTGATTCTGGAA
CTGCTGTACCGGCAAAAGCGCGCCAATAAATCAACGCTGGCCCGGCTGGCGCAAATTTCG
ATTCCGGCAGTCAGTAATATTTTGCAGGAACTGGAAAGCGAAAAACGGGTGGTGAATATT
GACGATGAAAGCCAGACGCGCGGGCATAGTAGCGGTACATGGCTGATTGCGCCGGAAGGT
GACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCT
TGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCG
CTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACC
ATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAACC
ATGCCGCAAGCGCCGTGGACAACGCCGGTGGAGGTGAAATATCTGCTGGAAGAGAAGCTC
GGCATTCGGGTGATGGTCGATAATGACTGCGTGATGCTGGCGCTGGCGGAGAAATGGCAA
AATAATTCGCAGGAACGGGATTTCTGCGTGATCAACGTTGATTACGGCATTGGCTCGTCG
TTCGTGATTAACGAGCAAATTTATCGCGGCAGTTTGTATGGTAGCGGACAGATTGGTCAC
ACCATCGTTAATCCGGATGGCGTCGTCTGCGACTGTGGACGTTATGGCTGCCTGGAAACT
GTCGCCTCGTTAAGCGCATTAAAAAAACAGGCGCGGGTATGGCTAAAATCACAACCGGTT
AGTACTCAACTTGATCCTGAAAAACTGACTACAGCGCAGTTAATCGCTGCCTGGCAAAGT
GGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTG
TATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAGTTGTGCC
TTTGGTGAGAACTGGCTTAATACTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGC
GACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAA
CAGGTGCTGGGAATTGGCTATTTGTATGTTGAGGCGCAGTTACGACAGATTTGA

Protein Properties

Pfam Domain Function:

Aminotran_1_2 (PF00155 )

Protein Residues:

397

Protein Molecular Weight:

43537

Protein Theoretical pI:

PDB File:

3TAT

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Aromatic-amino-acid aminotransferase
MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIPQLQAVAEAEARLNAQP
HGASLYLPMEGLNCYRHAIAPLLFGADHPVLKQQRVATIQTLGGSGALKVGADFLKRYFP
ESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRFNDLLATLKTLPARSIVLLHP
CCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGFGAGMEEDAYAIRAIASAGLPAL
VSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQLKATVRRNYSSPPNFGAQVVAAVLN
DEALKASWLAEVEEMRTRILAMRQELVKVLSTEMPERNFDYLLNQRGMFSYTGLSAAQVD
RLREEFGVYLIASGRMCVAGLNTANVQRVAKAFAAVM

References

External Links:

Resource	Link
Uniprot ID:	P04693
Uniprot Name:	TYRB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85675445
PDB ID:	3TAT
Ecogene ID:	EG11040
Ecocyc:	EG11040
ColiBase:	b4054
Kegg Gene:	b4054
EchoBASE ID:	EB1033
CCDB:	TYRB_ECOLI
BacMap:	16131880

General Reference:

Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fotheringham, I. G., Dacey, S. A., Taylor, P. P., Smith, T. J., Hunter, M. G., Finlay, M. E., Primrose, S. B., Parker, D. M., Edwards, R. M. (1986). "The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes." Biochem J 234:593-604. Pubmed: 3521591
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Ko, T. P., Wu, S. P., Yang, W. Z., Tsai, H., Yuan, H. S. (1999). "Crystallization and preliminary crystallographic analysis of the Escherichia coli tyrosine aminotransferase." Acta Crystallogr D Biol Crystallogr 55:1474-1477. Pubmed: 10417420
Kuramitsu, S., Inoue, K., Ogawa, T., Ogawa, H., Kagamiyama, H. (1985). "Aromatic amino acid aminotransferase of Escherichia coli: nucleotide sequence of the tyrB gene." Biochem Biophys Res Commun 133:134-139. Pubmed: 3907634
Yang, J., Pittard, J. (1987). "Molecular analysis of the regulatory region of the Escherichia coli K-12 tyrB gene." J Bacteriol 169:4710-4715. Pubmed: 3308851

Aromatic-amino-acid aminotransferase (P04693)