Maltoporin (P02943)

Identification
Name:Maltoporin
Synonyms:
  • Lambda receptor protein
  • Maltose-inducible porin
Gene Name:lamB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in porin activity
Specific Function:Involved in the transport of maltose and maltodextrins, indispensable for translocation of dextrins containing more than three glucosyl moieties. A hydrophobic path ("greasy slide") of aromatic residues serves to guide and select the sugars for transport through the channel. Also acts as a receptor for several bacteriophages including lambda
Cellular Location:Cell outer membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB211101,4-alpha-D-glucanMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB00163D-MaltoseMetaboCard
ECMDB21243MaltohexaoseMetaboCard
ECMDB21244MaltopentaoseMetaboCard
ECMDB01296MaltotetraoseMetaboCard
ECMDB01262MaltotrioseMetaboCard
GO Classification:
Component
cell part
membrane
Function
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b4036
Gene OrientationClockwise
Centisome Percentage:91.51
Left Sequence End4245994
Right Sequence End4247334
Gene Sequence:
>1341 bp
GTGACTACATTAACGCTTGTTTTAACAGCAGTAGGGTCTGTTTTACTGCTGCTGTTTTTA
GTCATGAAGGCGCGTATGCACGCTTTCCTGGCTTTAATGGTGGTGTCCATGGGGGCTGGC
CTTTTTTCTGGTATGCCGCTCGATAAAATCGCAGCGACGATGGAAAAAGGGATGGGAGGC
ACCCTCGGCTTCCTGGCGGTGGTTGTCGCCCTGGGAGCTATGTTTGGCAAGATCTTACAT
GAAACCGGCGCAGTCGATCAGATTGCCGTCAAAATGCTCAAATCCTTCGGTCACAGCCGC
GCGCATTATGCCATCGGCCTTGCGGGGCTGGTCTGTGCGCTACCGCTGTTCTTTGAAGTG
GCGATAGTTCTGCTGATTAGCGTTGCTTTCTCAATGGCGCGCCACACCGGTACGAACCTG
GTGAAGCTGGTAATCCCATTATTTGCAGGCGTGGCGGCAGCGGCGGCATTTCTGGTGCCT
GGACCAGCGCCGATGCTGCTGGCATCGCAGATGAATGCCGACTTTGGCTGGATGATCCTG
ATTGGCCTGTGTGCGGCAATTCCGGGAATGATTATTGCCGGGCCGCTGTGGGGTAACTTC
ATCAGCCGTTACGTTGAGCTGCATATTCCTGACGACATCAGCGAACCGCATCTCGGCGAA
GGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTA
GGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAGGATCAACTGCTTACGAATGGTTT
GAGTTTATCGGTCATCCGTTTACCGCGATTCTGGTTGCTTGTCTGGTGGCGATTTACGGT
CTGGCAATGCGTCAGGGCATGCCAAAAGACAAAGTGATGGAGATTTGCGGTCACGCGCTG
CAACCGGCGGGGATCATTCTGCTGGTGATTGGTGCGGGCGGCGTGTTCAAACAGGTGCTG
GTTGACTCTGGCGTAGGTCCGGCACTGGGCGAAGCGTTAACCGGCATGGGCCTGCCGATT
GCTATCACCTGCTTCGTGCTGGCAGCTGCAGTGCGCATCATTCAGGGTTCTGCCACCGTA
GCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAACAACTGAACTACTCCGGT
GCGCAAATGGCGGCGCTGTCGATTTGTATCGCTGGTGGTTCGATTGTTGTCAGCCACGTT
AACGACGCCGGTTTCTGGTTGTTCGGTAAATTTACCGGCGCGACCGAAGCCGAAACGCTG
AAAACCTGGACCATGATGGAAACCATCCTCGGCACTGTCGGTGCCATCGTTGGGATGATT
GCGTTCCAGCTGTTGAGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:446
Protein Molecular Weight:49912
Protein Theoretical pI:5
PDB File:1MPQ
Signaling Regions:
  • 1-25
Transmembrane Regions:
  • 27-35
  • 65-78
  • 81-93
  • 105-115
  • 123-130
  • 149-159
  • 162-173
  • 192-205
  • 210-222
  • 235-248
  • 251-263
  • 291-305
  • 308-323
  • 327-341
  • 344-359
  • 363-378
  • 386-400
  • 431-445
Protein Sequence:
>Maltoporin
MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGEQQCFQTTGAQSKYRLG
NECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEATDPAFREANVQGKNLIEWL
PGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVGFGKLSLAATRSSEAGGSSSF
ASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDYGRANLRDNYRLVDGASKDGWLF
TAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSGVAFDNEKFAYNINNNGHMLRILDH
GAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVGIRPMYKWTPIMSTVMEIGYDNVESQR
TGDKNNQYKITLAQQWQAGDSIWSRPAIRVFATYAKWDEKWGYDYTGNADNNANFGKAVP
ADFNGGSFGRGDSDEWTFGAQMEIWW
References
External Links:
ResourceLink
Uniprot ID:P02943
Uniprot Name:LAMB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676607
PDB ID:1MPQ
Ecogene ID:EG10528
Ecocyc:EG10528
ColiBase:b4036
Kegg Gene:b4036
EchoBASE ID:EB0523
CCDB:LAMB_ECOLI
BacMap:16131862
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Charbit, A., Ronco, J., Michel, V., Werts, C., Hofnung, M. (1991). "Permissive sites and topology of an outer membrane protein with a reporter epitope." J Bacteriol 173:262-275. Pubmed: 1702781
  • Clement, J. M., Hedgpeth, J., Hofnung, M. (1979). "[DNA sequence encoding the signal peptide of the lambda receptor in E. coli K 12]." C R Seances Acad Sci D 289:1033-1036. Pubmed: 161974
  • Clement, J. M., Hofnung, M. (1981). "Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12." Cell 27:507-514. Pubmed: 6086106
  • Dutzler, R., Wang, Y. F., Rizkallah, P., Rosenbusch, J. P., Schirmer, T. (1996). "Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway." Structure 4:127-134. Pubmed: 8805519
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hedgpeth, J., Clement, J. M., Marchal, C., Perrin, D., Hofnung, M. (1980). "DNA sequence encoding the NH2-terminal peptide involved in transport of lambda receptor, an Escherichia coli secretory protein." Proc Natl Acad Sci U S A 77:2621-2625. Pubmed: 6446717
  • Heine, H. G., Francis, G., Lee, K. S., Ferenci, T. (1988). "Genetic analysis of sequences in maltoporin that contribute to binding domains and pore structure." J Bacteriol 170:1730-1738. Pubmed: 2832377
  • Heine, H. G., Kyngdon, J., Ferenci, T. (1987). "Sequence determinants in the lamB gene of Escherichia coli influencing the binding and pore selectivity of maltoporin." Gene 53:287-292. Pubmed: 3301537
  • Klebba, P. E., Hofnung, M., Charbit, A. (1994). "A model of maltodextrin transport through the sugar-specific porin, LamB, based on deletion analysis." EMBO J 13:4670-4675. Pubmed: 7925308
  • Luckey, M., Ling, R., Dose, A., Malloy, B. (1991). "Role of a disulfide bond in the thermal stability of the LamB protein trimer in Escherichia coli outer membrane." J Biol Chem 266:1866-1871. Pubmed: 1988451
  • Schirmer, T., Keller, T. A., Wang, Y. F., Rosenbusch, J. P. (1995). "Structural basis for sugar translocation through maltoporin channels at 3.1 A resolution." Science 267:512-514. Pubmed: 7824948
  • Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137
  • Van Gelder, P., Dutzler, R., Dumas, F., Koebnik, R., Schirmer, T. (2001). "Sucrose transport through maltoporin mutants of Escherichia coli." Protein Eng 14:943-948. Pubmed: 11742115
  • Wang, Y. F., Dutzler, R., Rizkallah, P. J., Rosenbusch, J. P., Schirmer, T. (1997). "Channel specificity: structural basis for sugar discrimination and differential flux rates in maltoporin." J Mol Biol 272:56-63. Pubmed: 9299337