Anthranilate synthase component 1 (P00895)

Identification
Name:Anthranilate synthase component 1
Synonyms:
  • Anthranilate synthase component I
Gene Name:trpE
Enzyme Class:
Biological Properties
General Function:Involved in biosynthetic process
Specific Function:Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0L-Glutamine ↔ 1.02-Aminobenzoic acid + 1.0L-Glutamate + 1.0Hydrogen ion + 1.0Pyruvic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0Ammonia ↔ 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0Water
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0L-Glutamine ↔ 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0L-Glutamine → 1.0Hydrogen ion + 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0L-Glutamic acid+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0L-Glutamine → 1.0L-Glutamic acid + 1.0Pyruvic acid + 1.0Hydrogen ion + 1.02-Aminobenzoic acid + 1.0L-Glutamate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0L-Glutamine ↔ 1.02-Aminobenzoic acid + 1.0L-Glutamate + 1.0Hydrogen ion + 1.0Pyruvic acid
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0L-Glutamine → 1.0Hydrogen ion + 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Chorismate + 1.0L-Glutamine → 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB011232-Aminobenzoic acidMetaboCard
ECMDB00051AmmoniaMetaboCard
ECMDB12199ChorismateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB00641L-GlutamineMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
anthranilate synthase activity
carbon-carbon lyase activity
catalytic activity
lyase activity
oxo-acid-lyase activity
Process
biosynthetic process
metabolic process
Gene Properties
Blattner:b1264
Gene OrientationCounterclockwise
Centisome Percentage:28.44
Left Sequence End1319408
Right Sequence End1320970
Gene Sequence:
>1563 bp
ATGCAAACACAAAAACCGACTCTCGAACTGCTAACCTGCGAAGGCGCTTATCGCGACAAT
CCCACCGCGCTTTTTCACCAGTTGTGTGGGGATCGTCCGGCAACGCTGCTGCTGGAATCC
GCAGATATCGACAGCAAAGATGATTTAAAAAGCCTGCTGCTGGTAGACAGTGCGCTGCGC
ATTACAGCTTTAGGTGACACTGTCACAATCCAGGCACTTTCCGGCAACGGCGAAGCCCTC
CTGGCACTACTGGATAACGCCCTGCCTGCGGGTGTGGAAAGTGAACAATCACCAAACTGC
CGTGTGCTGCGCTTCCCCCCTGTCAGTCCACTGCTGGATGAAGACGCCCGCTTATGCTCC
CTTTCGGTTTTTGACGCTTTCCGTTTATTGCAGAATCTGTTGAATGTACCGAAGGAAGAA
CGAGAAGCCATGTTCTTCGGCGGCCTGTTCTCTTATGACCTTGTGGCGGGATTTGAAGAT
TTACCGCAACTGTCAGCGGAAAATAACTGCCCTGATTTCTGTTTTTATCTCGCTGAAACG
CTGATGGTGATTGACCATCAGAAAAAAAGCACCCGTATTCAGGCCAGCCTGTTTGCTCCG
AATGAAGAAGAAAAACAACGTCTCACTGCTCGCCTGAACGAACTACGTCAGCAACTGACC
GAAGCCGCGCCGCCGCTGCCAGTGGTTTCCGTGCCGCATATGCGTTGTGAATGTAATCAG
AGCGATGAAGAGTTCGGTGGCGTAGTGCGTTTGTTGCAAAAAGCGATTCGCGCTGGAGAA
ATTTTCCAGGTGGTGCCATCTCGCCGTTTCTCTCTGCCCTGCCCGTCACCGCTGGCGGCC
TATTACGTGCTGAAAAAGAGTAATCCCAGCCCGTACATGTTTTTTATGCAGGATAATGAT
TTCACCCTATTTGGCGCGTCGCCGGAAAGCTCGCTCAAGTATGATGCCACCAGCCGCCAG
ATTGAGATCTACCCGATTGCCGGAACACGCCCACGCGGTCGTCGCGCCGATGGTTCACTG
GACAGAGATCTCGACAGCCGTATTGAACTGGAAATGCGTACCGATCATAAAGAGCTGTCT
GAACATCTGATGCTGGTTGATCTCGCCCGTAATGATCTGGCACGCATTTGCACCCCCGGC
AGCCGCTACGTCGCCGATCTCACCAAAGTTGACCGTTATTCCTATGTGATGCACCTCGTC
TCTCGCGTAGTCGGCGAACTGCGTCACGATCTTGACGCCCTGCACGCTTATCGCGCCTGT
ATGAATATGGGGACGTTAAGCGGTGCGCCGAAAGTACGCGCTATGCAGTTAATTGCCGAG
GCGGAAGGTCGTCGCCGCGGCAGCTACGGCGGCGCGGTAGGTTATTTCACCGCGCATGGC
GATCTCGACACCTGCATTGTGATCCGCTCGGCGCTGGTGGAAAACGGTATCGCCACCGTG
CAAGCGGGTGCTGGTGTAGTCCTTGATTCTGTTCCGCAGTCGGAAGCCGACGAAACCCGT
AACAAAGCCCGCGCTGTACTGCGCGCTATTGCCACCGCGCATCATGCACAGGAGACTTTC
TGA
Protein Properties
Pfam Domain Function:
Protein Residues:520
Protein Molecular Weight:57494
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Anthranilate synthase component 1
MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALR
ITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRVLRFPPVSPLLDEDARLCS
LSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
LMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQLTEAAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENGIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF
References
External Links:
ResourceLink
Uniprot ID:P00895
Uniprot Name:TRPE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742057
Ecogene ID:EG11028
Ecocyc:EG11028
ColiBase:b1264
Kegg Gene:b1264
EchoBASE ID:EB1021
CCDB:TRPE_ECOLI
BacMap:16129225
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lee, F., Bertrand, K., Bennett, G., Yanofsky, C. (1978). "Comparison of the nucleotide sequences of the initial transcribed regions of the tryptophan operons of Escherichia coli and Salmonella typhimurium." J Mol Biol 121:193-217. Pubmed: 351195
  • Li, S. L., Hanlon, J., Yanofsky, C. (1974). "Separation of anthranilate synthetase components I and II of Escherichia coli, Salmonella typhimurium, and Serratia marcescens and determination of their amino-terminal sequences by automatic Edman degradation." Biochemistry 13:1736-1744. Pubmed: 4598537
  • Nichols, B. P., van Cleemput, M., Yanofsky, C. (1981). "Nucleotide sequence of Escherichia coli trpE. Anthranilate synthetase component I contains no tryptophan residues." J Mol Biol 146:45-54. Pubmed: 7021857
  • Yanofsky, C., Platt, T., Crawford, I. P., Nichols, B. P., Christie, G. E., Horowitz, H., VanCleemput, M., Wu, A. M. (1981). "The complete nucleotide sequence of the tryptophan operon of Escherichia coli." Nucleic Acids Res 9:6647-6668. Pubmed: 7038627