ECMDB: Anthranilate synthase component 1 (P00895)

Identification

Name:

Anthranilate synthase component 1

Synonyms:

Anthranilate synthase component I

Gene Name:

trpE

Enzyme Class:

4.1.3.27

Biological Properties

General Function:

Involved in biosynthetic process

Specific Function:

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate

Cellular Location:

Not Available

SMPDB Pathways:

Trp Operon PW000965
Tryptophan metabolism PW000815
tryptophan metabolism II PW001916

KEGG Pathways:

Metabolic pathways eco01100
Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
Tryptophan metabolism ec00380

KEGG Reactions:

1.0

↔

1.0

1.0Chorismate + 1.0L-Glutamine ↔ 1.02-Aminobenzoic acid + 1.0L-Glutamate + 1.0Hydrogen ion + 1.0Pyruvic acid
ReactionCard

1.0

↔

1.0

1.0Chorismate + 1.0Ammonia ↔ 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0Water
ReactionCard

1.0

↔

1.0

1.0Chorismate + 1.0L-Glutamine ↔ 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard

1.0

→

1.0

1.0Chorismate + 1.0L-Glutamine → 1.0Hydrogen ion + 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard

SMPDB Reactions:

1.0

→

1.0L-Glutamic acid

1.0

1.0Chorismate + 1.0L-Glutamine → 1.0L-Glutamic acid + 1.0Pyruvic acid + 1.0Hydrogen ion + 1.02-Aminobenzoic acid + 1.0L-Glutamate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Chorismate + 1.0L-Glutamine ↔ 1.02-Aminobenzoic acid + 1.0L-Glutamate + 1.0Hydrogen ion + 1.0Pyruvic acid
ReactionCard

1.0

→

1.0

1.0Chorismate + 1.0L-Glutamine → 1.0Hydrogen ion + 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Chorismate + 1.0L-Glutamine → 1.02-Aminobenzoic acid + 1.0Pyruvic acid + 1.0L-Glutamate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01123	2-Aminobenzoic acid	MetaboCard
ECMDB00051	Ammonia	MetaboCard
ECMDB12199	Chorismate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB00641	L-Glutamine	MetaboCard
ECMDB00243	Pyruvic acid	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
anthranilate synthase activity
carbon-carbon lyase activity
catalytic activity
lyase activity
oxo-acid-lyase activity
Process
biosynthetic process
metabolic process

Gene Properties

Blattner:

b1264

Gene Orientation

Counterclockwise

Centisome Percentage:

28.44

Left Sequence End

1319408

Right Sequence End

1320970

Gene Sequence:

>1563 bp
ATGCAAACACAAAAACCGACTCTCGAACTGCTAACCTGCGAAGGCGCTTATCGCGACAAT
CCCACCGCGCTTTTTCACCAGTTGTGTGGGGATCGTCCGGCAACGCTGCTGCTGGAATCC
GCAGATATCGACAGCAAAGATGATTTAAAAAGCCTGCTGCTGGTAGACAGTGCGCTGCGC
ATTACAGCTTTAGGTGACACTGTCACAATCCAGGCACTTTCCGGCAACGGCGAAGCCCTC
CTGGCACTACTGGATAACGCCCTGCCTGCGGGTGTGGAAAGTGAACAATCACCAAACTGC
CGTGTGCTGCGCTTCCCCCCTGTCAGTCCACTGCTGGATGAAGACGCCCGCTTATGCTCC
CTTTCGGTTTTTGACGCTTTCCGTTTATTGCAGAATCTGTTGAATGTACCGAAGGAAGAA
CGAGAAGCCATGTTCTTCGGCGGCCTGTTCTCTTATGACCTTGTGGCGGGATTTGAAGAT
TTACCGCAACTGTCAGCGGAAAATAACTGCCCTGATTTCTGTTTTTATCTCGCTGAAACG
CTGATGGTGATTGACCATCAGAAAAAAAGCACCCGTATTCAGGCCAGCCTGTTTGCTCCG
AATGAAGAAGAAAAACAACGTCTCACTGCTCGCCTGAACGAACTACGTCAGCAACTGACC
GAAGCCGCGCCGCCGCTGCCAGTGGTTTCCGTGCCGCATATGCGTTGTGAATGTAATCAG
AGCGATGAAGAGTTCGGTGGCGTAGTGCGTTTGTTGCAAAAAGCGATTCGCGCTGGAGAA
ATTTTCCAGGTGGTGCCATCTCGCCGTTTCTCTCTGCCCTGCCCGTCACCGCTGGCGGCC
TATTACGTGCTGAAAAAGAGTAATCCCAGCCCGTACATGTTTTTTATGCAGGATAATGAT
TTCACCCTATTTGGCGCGTCGCCGGAAAGCTCGCTCAAGTATGATGCCACCAGCCGCCAG
ATTGAGATCTACCCGATTGCCGGAACACGCCCACGCGGTCGTCGCGCCGATGGTTCACTG
GACAGAGATCTCGACAGCCGTATTGAACTGGAAATGCGTACCGATCATAAAGAGCTGTCT
GAACATCTGATGCTGGTTGATCTCGCCCGTAATGATCTGGCACGCATTTGCACCCCCGGC
AGCCGCTACGTCGCCGATCTCACCAAAGTTGACCGTTATTCCTATGTGATGCACCTCGTC
TCTCGCGTAGTCGGCGAACTGCGTCACGATCTTGACGCCCTGCACGCTTATCGCGCCTGT
ATGAATATGGGGACGTTAAGCGGTGCGCCGAAAGTACGCGCTATGCAGTTAATTGCCGAG
GCGGAAGGTCGTCGCCGCGGCAGCTACGGCGGCGCGGTAGGTTATTTCACCGCGCATGGC
GATCTCGACACCTGCATTGTGATCCGCTCGGCGCTGGTGGAAAACGGTATCGCCACCGTG
CAAGCGGGTGCTGGTGTAGTCCTTGATTCTGTTCCGCAGTCGGAAGCCGACGAAACCCGT
AACAAAGCCCGCGCTGTACTGCGCGCTATTGCCACCGCGCATCATGCACAGGAGACTTTC
TGA

Protein Properties

Pfam Domain Function:

Anth_synt_I_N (PF04715 )
Chorismate_bind (PF00425 )

Protein Residues:

520

Protein Molecular Weight:

57494

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Anthranilate synthase component 1
MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALR
ITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRVLRFPPVSPLLDEDARLCS
LSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
LMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQLTEAAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENGIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF

References

External Links:

Resource	Link
Uniprot ID:	P00895
Uniprot Name:	TRPE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1742057
Ecogene ID:	EG11028
Ecocyc:	EG11028
ColiBase:	b1264
Kegg Gene:	b1264
EchoBASE ID:	EB1021
CCDB:	TRPE_ECOLI
BacMap:	16129225

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Lee, F., Bertrand, K., Bennett, G., Yanofsky, C. (1978). "Comparison of the nucleotide sequences of the initial transcribed regions of the tryptophan operons of Escherichia coli and Salmonella typhimurium." J Mol Biol 121:193-217. Pubmed: 351195
Li, S. L., Hanlon, J., Yanofsky, C. (1974). "Separation of anthranilate synthetase components I and II of Escherichia coli, Salmonella typhimurium, and Serratia marcescens and determination of their amino-terminal sequences by automatic Edman degradation." Biochemistry 13:1736-1744. Pubmed: 4598537
Nichols, B. P., van Cleemput, M., Yanofsky, C. (1981). "Nucleotide sequence of Escherichia coli trpE. Anthranilate synthetase component I contains no tryptophan residues." J Mol Biol 146:45-54. Pubmed: 7021857
Yanofsky, C., Platt, T., Crawford, I. P., Nichols, B. P., Christie, G. E., Horowitz, H., VanCleemput, M., Wu, A. M. (1981). "The complete nucleotide sequence of the tryptophan operon of Escherichia coli." Nucleic Acids Res 9:6647-6668. Pubmed: 7038627