2,5-diketo-D-gluconic acid reductase A (Q46857)

Identification
Name:2,5-diketo-D-gluconic acid reductase A
Synonyms:
  • 2,5-DKG reductase A
  • 2,5-DKGR A
  • 25DKGR-A
  • AKR5C
Gene Name:dkgA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0NADP ↔ 1.02,5-Diketo-D-gluconate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Dehydro-D-gluconate + 1.0NADP ↔ 1.02,5-Diketo-D-gluconate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0NADPH+1.0Thumb → 1.0Thumb+1.02-Keto-L-gluconate+1.0Thumb
1.02,5-Diketo-D-gluconate + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.02-Keto-L-gluconate + 1.02-Dehydro-D-gluconate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Hydroxyacetone+1.0Thumb
1.0Pyruvaldehyde + 1.0NADPH + 1.0Hydrogen ion ↔ 1.0Hydroxyacetone + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Pyruvaldehyde + 1.0NADPH + 1.0Hydrogen ion → 1.0Lactaldehyde + 1.0NADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADPH + 1.02,5-Diketo-D-gluconate ↔ 1.02-Dehydro-D-gluconate + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Ethyl-2-methylacetoacetate + 1.0NADPH ↔ 1.0Ethyl-(2R)-methyl-(3S)-hydroxybutanoate + 1.0NADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 2.0-Dehydro-L-gulonate+1.0Thumb
1.02,5-Diketo-D-gluconate + 1.0Hydrogen ion + 1.0NADPH → 2.0-Dehydro-L-gulonate + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Acetol+1.0Thumb
1.0Hydrogen ion + 1.0Pyruvaldehyde + 1.0NADPH → 1.0Acetol + 1.0NADP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB040622,5-Diketo-D-gluconateMetaboCard
ECMDB040422-Dehydro-D-gluconateMetaboCard
ECMDB20554Ethyl-(2R)-methyl-(3S)-hydroxybutanoateMetaboCard
ECMDB20553Ethyl-2-methylacetoacetateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB03052LactaldehydeMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB01167PyruvaldehydeMetaboCard
GO Classification:
Function
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b3012
Gene OrientationClockwise
Centisome Percentage:67.99
Left Sequence End3154645
Right Sequence End3155472
Gene Sequence:
>828 bp
ATGAGTGTGCTCATTAATGAAAAACTGCATTCGCGGCGGCTGAAATGGCGCTGGCCGCTC
TCGCGTCAGGTGACCTTAAGCATTGGCACGTTAGCGGTTTTACTCACCGTATGGTGGACG
GTGGCGACGCTGCAACTGATTAGCCCGCTATTTTTGCCGCCGCCGCAACAGGTACTGGAA
AAACTACTCACCATTGCCGGACCGCAAGGCTTTATGGACGCCACGCTGTGGCAGCATCTG
GCAGCCAGTCTGACGCGCATTATGCTGGCGCTATTTGCAGCGGTGTTGTTCGGTATTCCG
GTCGGGATCGCGATGGGACTTAGCCCTACGGTACGCGGCATTCTGGATCCGATAATCGAG
CTTTATCGTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATT
GGTGAAACCTCGAAGATCTTACTGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCG
GCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGTTCGCATTCGTGCCGCCCAGTCGCTGGGT
GCCAGCCGTGCGCAGGTGCTGTGGTTTGTCATTTTGCCCGGTGCGCTGCCGGAAATCCTC
ACCGGATTACGTATTGGTCTGGGGGTGGGCTGGTCTACGCTGGTGGCGGCGGAGCTGATT
GCCGCGACGCGCGGTTTAGGATTTATGGTTCAGTCAGCGGGTGAATTTCTCGCAACTGAC
GTGGTGCTGGCGGGGATCGCGGTGATTGCGATTATCGCCTTTCTTTTAGAACTGGGTCTG
CGCGCGTTACAGCGCCGCCTGACGCCCTGGCATGGAGAAGTACAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:275
Protein Molecular Weight:31109
Protein Theoretical pI:6
PDB File:1MZR
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2,5-diketo-D-gluconic acid reductase A
MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRSIDTAAAYKNEEGVGKA
LKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYIDLYLMHWPVPAIDHYVEAW
KGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQIELHPLMQQRQLHAWNATHK
IQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVIRWHLDSGLVVIPKSVTPSRIAE
NFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG
References
External Links:
ResourceLink
Uniprot ID:Q46857
Uniprot Name:DKGA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674508
PDB ID:1MZR
Ecogene ID:EG13015
Ecocyc:EG13015
ColiBase:b3012
Kegg Gene:b3012
EchoBASE ID:EB2835
CCDB:DKGA_ECOLI
BacMap:90111527
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Habrych, M., Rodriguez, S., Stewart, J. D. (2002). "Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE." Biotechnol Prog 18:257-261. Pubmed: 11934293
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jeudy, S., Monchois, V., Maza, C., Claverie, J. M., Abergel, C. (2006). "Crystal structure of Escherichia coli DkgA, a broad-specificity aldo-keto reductase." Proteins 62:302-307. Pubmed: 16284956
  • Yum, D. Y., Lee, B. Y., Pan, J. G. (1999). "Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli." Appl Environ Microbiol 65:3341-3346. Pubmed: 10427017