CTP pyrophosphohydrolase (P77788)

Identification
Name:CTP pyrophosphohydrolase
Synonyms:Not Available
Gene Name:nudG
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order
Cellular Location:Cytoplasmic
SMPDB Pathways:
  • Collection of Reactions without pathways PW001891
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Cytidine triphosphate + 1.0Water → 1.0Cytidine monophosphate + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Cytidine triphosphate + 1.0Water + 1.0dCTP ↔ 1.0Cytidine monophosphate + 1.0Pyrophosphate + 1.0dCMP
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.02-hydroxy-dAMP
1.0Water + 1.02-Hydroxydeoxyadenosine 5'-triphosphate → 1.0Pyrophosphate + 1.02-hydroxy-dAMP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Cytidine triphosphate + 1.0Water → 1.0Cytidine monophosphate + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0dCTP + 1.0Water → 1.0dCMP + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.02-hydroxydeoxyadenosine 5'-monophosphate+1.0Thumb
1.02-Hydroxydeoxyadenosine 5'-triphosphate + 1.0Water → 1.02-hydroxydeoxyadenosine 5'-monophosphate + 1.0Pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Cytidine triphosphate + 1.0Water → 1.0Cytidine monophosphate + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB202262-Hydroxydeoxyadenosine 5'-triphosphateMetaboCard
ECMDB215015-Methyldeoxycytidine triphosphateMetaboCard
ECMDB202468-Hydroxydeoxyguanosine 5'-triphosphateMetaboCard
ECMDB00095Cytidine monophosphateMetaboCard
ECMDB00082Cytidine triphosphateMetaboCard
ECMDB01202dCMPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
Gene Properties
Blattner:b1759
Gene OrientationClockwise
Centisome Percentage:39.65
Left Sequence End1839514
Right Sequence End1839921
Gene Sequence:
>408 bp
ATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTAT
GAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACG
CAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTG
GAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTC
AAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCC
ATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGC
CCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:135
Protein Molecular Weight:15046
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>CTP pyrophosphohydrolase
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDESQRQALVRELREELGIE
ATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIP
LLEAFMALRAARPAD
References
External Links:
ResourceLink
Uniprot ID:P77788
Uniprot Name:NUDG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674626
Ecogene ID:EG14009
Ecocyc:EG14009
ColiBase:b1759
Kegg Gene:b1759
EchoBASE ID:EB3765
CCDB:NUDG_ECOLI
BacMap:16129713
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • O'Handley, S. F., Dunn, C. A., Bessman, M. J. (2001). "Orf135 from Escherichia coli Is a Nudix hydrolase specific for CTP, dCTP, and 5-methyl-dCTP." J Biol Chem 276:5421-5426. Pubmed: 11053429