Allantoinase (P77671)

Identification
Name:Allantoinase
Synonyms:
  • Allantoin-utilizing enzyme
Gene Name:allB
Enzyme Class:
Biological Properties
General Function:Involved in allantoinase activity
Specific Function:Catalyzes the conversion of allantoin (5- ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0(S)(+)-Allantoin + 1.0Water ↔ 1.0Allantoic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Allantoin + 1.0Water → 1.0Allantoic acid + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb
1.0(S)(+)-Allantoin + 1.0Water → 1.0Allantoic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20018(S)(+)-AllantoinMetaboCard
ECMDB01209Allantoic acidMetaboCard
ECMDB00462AllantoinMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
allantoinase activity
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
ion binding
metal ion binding
transition metal ion binding
zinc ion binding
Process
allantoin catabolic process
allantoin metabolic process
amine metabolic process
metabolic process
nitrogen compound metabolic process
Gene Properties
Blattner:b0512
Gene OrientationClockwise
Centisome Percentage:11.60
Left Sequence End538371
Right Sequence End539732
Gene Sequence:
>1362 bp
ATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTT
GTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCA
AAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACC
CATATTTCTGAACCGGGTCGTAGCCACTGGGAAGGTTATGAAACCGGTACTCGCGCAGCG
GCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTGCCTGCAACGGTT
GACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCG
GCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTG
GGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGAC
TTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGT
CAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCG
AAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAA
GTGGAAGCAATTCGCCGCGTACTGTATCTGGCGAAAGTTGCTGGTTGCCGTCTGCACGTT
TGCCACGTCAGCAGCCCGGAAGGTGTTGAGGAAGTGACTCGTGCACGTCAGGAAGGTCAG
GACGTTACTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAA
ATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATG
TGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCG
CCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGC
TGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTC
GGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTGCAGCAAAAAGGCCGTATC
GCCCCAGGAAAAGATGCCGACTTCGTCTTCATTCAGCCGAATAGCAGCTATGTTCTTACC
AATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCG
CGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCT
GTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:453
Protein Molecular Weight:49601
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Allantoinase
MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVMDASGLVVSPGMVDAHT
HISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDRASIELKFDAAKGKLTIDA
AQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDNDFRDVNDWQFFKGAQKLGELG
QPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVFTEVEAIRRVLYLAKVAGCRLHV
CHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTDQFEEIGTLAKCSPPIRDLENQKGM
WEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGGIAGLQSCMDVMFDEAVQKRGMSLPMF
GKLMATNAADIFGLQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEYRHKVSPYVGRTIGA
RITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ
References
External Links:
ResourceLink
Uniprot ID:P77671
Uniprot Name:ALLB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674650
Ecogene ID:EG13619
Ecocyc:EG13619
ColiBase:b0512
Kegg Gene:b0512
EchoBASE ID:EB3384
CCDB:ALLB_ECOLI
BacMap:16128496
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cusa, E., Obradors, N., Baldoma, L., Badia, J., Aguilar, J. (1999). "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." J Bacteriol 181:7479-7484. Pubmed: 10601204
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kim, G. J., Lee, D. E., Kim, H. S. (2000). "Functional expression and characterization of the two cyclic amidohydrolase enzymes, allantoinase and a novel phenylhydantoinase, from Escherichia coli." J Bacteriol 182:7021-7028. Pubmed: 11092864
  • Mulrooney, S. B., Hausinger, R. P. (2003). "Metal ion dependence of recombinant Escherichia coli allantoinase." J Bacteriol 185:126-134. Pubmed: 12486048