ECMDB: Succinylornithine transaminase (P77581)

Identification

Name:

Succinylornithine transaminase

Synonyms:

SOAT
Carbon starvation protein C
Succinylornithine aminotransferase

Gene Name:

astC

Enzyme Class:

2.6.1.81

Biological Properties

General Function:

Involved in transaminase activity

Specific Function:

Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase

Cellular Location:

Not Available

SMPDB Pathways:

arginine metabolism PW000790

KEGG Pathways:

Arginine and proline metabolism ec00330

KEGG Reactions:

1.0

↔

1.0

1.0N-Acetylornithine + 1.0alpha-Ketoglutarate ↔ 1.0N-Acetyl-L-glutamate 5-semialdehyde + 1.0L-Glutamate
ReactionCard

1.0

↔

1.0

1.0alpha-Ketoglutarate + 1.0N2-Succinyl-L-ornithine ↔ 1.0L-Glutamate + 1.0N2-Succinyl-L-glutamic acid 5-semialdehyde
ReactionCard

SMPDB Reactions:

1.0

→

1.0L-Glutamic acid

1.0

1.0N2-Succinyl-L-ornithine + 1.0Oxoglutaric acid → 1.0L-Glutamic acid + 1.0N2-Succinyl-L-glutamic acid 5-semialdehyde + 1.0L-Glutamate
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0N2-Succinyl-L-ornithine + 1.0Oxoglutaric acid → 1.0N2-Succinyl-L-glutamic acid 5-semialdehyde + 1.0L-Glutamate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB02812	alpha-Ketoglutarate	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB06488	N-Acetyl-L-glutamate 5-semialdehyde	MetaboCard
ECMDB03357	N-Acetylornithine	MetaboCard
ECMDB01180	N2-Succinyl-L-glutamic acid 5-semialdehyde	MetaboCard
ECMDB01199	N2-Succinyl-L-ornithine	MetaboCard
ECMDB04123	Oxoglutaric acid	MetaboCard

GO Classification:

Function
binding
catalytic activity
cofactor binding
pyridoxal phosphate binding
transaminase activity
transferase activity
transferase activity, transferring nitrogenous groups
Process
arginine metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
metabolic process

Gene Properties

Blattner:

b1748

Gene Orientation

Counterclockwise

Centisome Percentage:

39.42

Left Sequence End

1828786

Right Sequence End

1830006

Gene Sequence:

>1221 bp
ATGTTTAAGTCTTTTTTCCCAAAGCCGGGAACGTTTTTTCTCTCGGCCTTCGTTTGGGCA
TTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGC
GCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTT
TACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCG
CATCGTTGGCAATACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTT
TTGGTGGAAGTCGGGGTCGCCGTCAACGCCTGGTATGCGCCGTTCTATGATCTGATTCAA
ACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAATTTTACCGCGAAGTGGGCGTC
TTTCTGGGGATTGCGCTGATCGCTGTGGTGATCAGTGTGCTGAACAACTTCTTTGTCAGT
CACTACGTGTTCCGCTGGCGTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAA
CTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCA
ACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTG
CCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCG
TATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTG
GTAGGGATCAAACTGCCGGGGCTGGAGTTTAAAAACCAGCGTGTAGAGGCTGCCTACCGT
AAAGAGCTGGTTTATGGTGAAGACGATGCCACGCGCGCGACGCCGCCTACGGTACGCGAG
CTGTTTAGCGCCGTACGGAAAAACTATTTCCGCCTCTATTTTCACTATATGTATTTCAAC
ATCGCCCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCTTGCTGTTTCCG
TCAATTGTTGCCGGTACGATTACGCTCGGCCTGATGACGCAGATTACCAACGTTTTTGGT
CAGGTTCGCGGTGCTTTCCAGTACCTGATTAACTCATGGACCACACTGGTTGAGTTGATG
TCTATCTACAAACGTCTGCGCAGCTTTGAACATGAGCTGGATGGTGACAAAATTCAGGAA
GTAACCCATACCTTGAGCTAA

Protein Properties

Pfam Domain Function:

Aminotran_3 (PF00202 )

Protein Residues:

406

Protein Molecular Weight:

43665

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Succinylornithine transaminase
MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDFAGGIAVNALGHAHPEL
REALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFCNSGAEANEAALKLARKFA
HDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAPLPADIRHAAYNDINSASALI
DDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNALLIFDEVQTGVGRTGELYAYMH
YGVTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISQEAAK
AGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS

References

External Links:

Resource	Link
Uniprot ID:	P77581
Uniprot Name:	ASTC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674518
Ecogene ID:	EG13999
Ecocyc:	EG13999
ColiBase:	b1748
Kegg Gene:	b1748
EchoBASE ID:	EB3755
CCDB:	ASTC_ECOLI
BacMap:	16129702

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fraley, C. D., Kim, J. H., McCann, M. P., Matin, A. (1998). "The Escherichia coli starvation gene cstC is involved in amino acid catabolism." J Bacteriol 180:4287-4290. Pubmed: 9696780
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Schneider, B. L., Kiupakis, A. K., Reitzer, L. J. (1998). "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli." J Bacteriol 180:4278-4286. Pubmed: 9696779