Methylisocitrate lyase (P77541)

Identification
Name:Methylisocitrate lyase
Synonyms:
  • 2-methylisocitrate lyase
Gene Name:prpB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Methylisocitric acid ↔ 1.0Pyruvic acid + 1.0Succinic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Methylisocitric acid ↔ 1.0Pyruvic acid + 1.0Succinic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB06471Methylisocitric acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB00254Succinic acidMetaboCard
GO Classification:
Function
carbon-carbon lyase activity
catalytic activity
lyase activity
methylisocitrate lyase activity
oxo-acid-lyase activity
Process
carboxylic acid metabolic process
cellular metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
propionate catabolic process
propionate catabolic process, 2-methylcitrate cycle
propionate metabolic process
Gene Properties
Blattner:b0331
Gene OrientationClockwise
Centisome Percentage:7.50
Left Sequence End347906
Right Sequence End348796
Gene Sequence:
>891 bp
ATGAGCGGCTTACCTCTTATTTCGCGCCGTCGACTGTTAACGGCGATGGCGCTTTCTCCG
TTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCG
CTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCG
GATACCATCAACTATCGCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGAC
GTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATG
GTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTCGTATTGCGCCGGGTCGC
GGATTTAACTTCAGTGACGGCAAACAGCCGTTGGCGATGGCGCGTAAATCGCTGACGGAA
ATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGAC
TTTATCCGCAGCATGAAACCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACG
ACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCAGGAAATT
CTTGATGAGTACGGCATCCCAAATGCCTGGCAAGGGGAAACCAACTTCTGGGGCAGTACC
GCCGTCAGTATCGATCGTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCAC
GACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTT
GTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCG
GCAATGCACTTTGTGCGCGTTCTGGATAACGCCATCGGAGGTAAAGCGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:296
Protein Molecular Weight:32134
Protein Theoretical pI:5
PDB File:1OQF
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Methylisocitrate lyase
MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYLSGGGVAAGSLGLPDLG
ISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVKSMIKAGAAGLHIEDQVGA
KRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTDALAVEGLDAAIERAQAYVEA
GAEMLFPEAITELAMYRQFADAVQVPILANITEFGATPLFTTDELRSAHVAMALYPLSAF
RAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYESINYYQYEEKLDNLFARSQVK
References
External Links:
ResourceLink
Uniprot ID:P77541
Uniprot Name:PRPB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674358
PDB ID:1OQF
Ecogene ID:EG13601
Ecocyc:EG13601
ColiBase:b0331
Kegg Gene:b0331
EchoBASE ID:EB3370
CCDB:PRPB_ECOLI
BacMap:16128316
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Textor, S., Wendisch, V. F., De Graaf, A. A., Muller, U., Linder, M. I., Linder, D., Buckel, W. (1997). "Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria." Arch Microbiol 168:428-436. Pubmed: 9325432