1-deoxy-D-xylulose-5-phosphate synthase (P77488)

Identification
Name:1-deoxy-D-xylulose-5-phosphate synthase
Synonyms:
  • 1-deoxyxylulose-5-phosphate synthase
  • DXP synthase
  • DXPS
Gene Name:dxs
Enzyme Class:
Biological Properties
General Function:Involved in 1-deoxy-D-xylulose-5-phosphate synthase activity
Specific Function:Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary metabolites: isoprenoid biosynthesis (nonmevalonate pathway) PW000975
  • Secondary metabolites: methylerythritol phosphate and polyisoprenoid biosynthesis PW000958
  • Thiazole Biosynthesis I PW002041
  • Vitamin B6 1430936196 PW000891
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Glyceraldehyde 3-phosphate + 1.0Hydrogen ion + 1.0Pyruvic acid ↔ 1.0Carbon dioxide + 1.01-Deoxy-D-xylulose 5-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Pyruvic acid + 1.0D-Glyceraldehyde 3-phosphate ↔ 1.01-Deoxy-D-xylulose 5-phosphate + 1.0Carbon dioxide
ReactionCard
SMPDB Reactions:
1.0D-Glyceraldehyde 3-phosphate+1.0Thumb+1.0Thumb+1.0Thumb → 1.01-Deoxy-D-xylulose 5-phosphate+1.0Thumb+1.0Thumb
1.0D-Glyceraldehyde 3-phosphate + 1.0Pyruvic acid + 1.0Hydrogen ion + 1.0D-Glyceraldehyde 3-phosphate → 1.01-Deoxy-D-xylulose 5-phosphate + 1.0Carbon dioxide + 1.01-Deoxy-D-xylulose 5-phosphate
ReactionCard
1.0D-Glyceraldehyde 3-phosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.01-Deoxy-D-xylulose 5-phosphate+1.0Thumb
1.0D-Glyceraldehyde 3-phosphate + 1.0Hydrogen ion + 1.0D-Glyceraldehyde 3-phosphate → 1.0Carbon dioxide + 1.01-Deoxy-D-xylulose 5-phosphate + 1.01-Deoxy-D-xylulose 5-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Glyceraldehyde 3-phosphate + 1.0Hydrogen ion + 1.0Pyruvic acid ↔ 1.0Carbon dioxide + 1.01-Deoxy-D-xylulose 5-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Pyruvic acid + 1.0D-Glyceraldehyde 3-phosphate ↔ 1.01-Deoxy-D-xylulose 5-phosphate + 1.0Carbon dioxide
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Pyruvic acid + 1.0D-Glyceraldehyde 3-phosphate → 1.01-Deoxy-D-xylulose 5-phosphate + 1.0Carbon dioxide
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB012131-Deoxy-D-xylulose 5-phosphateMetaboCard
ECMDB04030Carbon dioxideMetaboCard
ECMDB01112D-Glyceraldehyde 3-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Function
1-deoxy-D-xylulose-5-phosphate synthase activity
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
transferase activity
transferase activity, transferring aldehyde or ketonic groups
Process
cellular lipid metabolic process
isoprenoid metabolic process
lipid metabolic process
metabolic process
primary metabolic process
terpenoid biosynthetic process
terpenoid metabolic process
Gene Properties
Blattner:b0420
Gene OrientationCounterclockwise
Centisome Percentage:9.43
Left Sequence End437539
Right Sequence End439401
Gene Sequence:
>1863 bp
ATGAGTTTTGATATTGCCAAATACCCGACCCTGGCACTGGTCGACTCCACCCAGGAGTTA
CGACTGTTGCCGAAAGAGAGTTTACCGAAACTCTGCGACGAACTGCGCCGCTATTTACTC
GACAGCGTGAGCCGTTCCAGCGGGCACTTCGCCTCCGGGCTGGGCACGGTCGAACTGACC
GTGGCGCTGCACTATGTCTACAACACCCCGTTTGACCAATTGATTTGGGATGTGGGGCAT
CAGGCTTATCCGCATAAAATTTTGACCGGACGCCGCGACAAAATCGGCACCATCCGTCAG
AAAGGCGGTCTGCACCCGTTCCCGTGGCGCGGCGAAAGCGAATATGACGTATTAAGCGTC
GGGCATTCATCAACCTCCATCAGTGCCGGAATTGGTATTGCGGTTGCTGCCGAAAAAGAA
GGCAAAAATCGCCGCACCGTCTGTGTCATTGGCGATGGCGCGATTACCGCAGGCATGGCG
TTTGAAGCGATGAATCACGCGGGCGATATCCGTCCTGATATGCTGGTGATTCTCAACGAC
AATGAAATGTCGATTTCCGAAAATGTCGGCGCGCTCAACAACCATCTGGCACAGCTGCTT
TCCGGTAAGCTTTACTCTTCACTGCGCGAAGGCGGGAAAAAAGTTTTCTCTGGCGTGCCG
CCAATTAAAGAGCTGCTCAAACGCACCGAAGAACATATTAAAGGCATGGTAGTGCCTGGC
ACGTTGTTTGAAGAGCTGGGCTTTAACTACATCGGCCCGGTGGACGGTCACGATGTGCTG
GGGCTTATCACCACGCTAAAGAACATGCGCGACCTGAAAGGCCCGCAGTTCCTGCATATC
ATGACCAAAAAAGGTCGTGGTTATGAACCGGCAGAAAAAGACCCGATCACTTTCCACGCC
GTGCCTAAATTTGATCCCTCCAGCGGTTGTTTGCCGAAAAGTAGCGGCGGTTTGCCGAGC
TATTCAAAAATCTTTGGCGACTGGTTGTGCGAAACGGCAGCGAAAGACAACAAGCTGATG
GCGATTACTCCGGCGATGCGTGAAGGTTCCGGCATGGTCGAGTTTTCACGTAAATTCCCG
GATCGCTACTTCGACGTGGCAATTGCCGAGCAACACGCGGTGACCTTTGCTGCGGGTCTG
GCGATTGGTGGGTACAAACCCATTGTCGCGATTTACTCCACTTTCCTGCAACGCGCCTAT
GATCAGGTGCTGCATGACGTGGCGATTCAAAAGCTTCCGGTCCTGTTCGCCATCGACCGC
GCGGGCATTGTTGGTGCTGACGGTCAAACCCATCAGGGTGCTTTTGATCTCTCTTACCTG
CGCTGCATACCGGAAATGGTCATTATGACCCCGAGCGATGAAAACGAATGTCGCCAGATG
CTCTATACCGGCTATCACTATAACGATGGCCCGTCAGCGGTGCGCTACCCGCGTGGCAAC
GCGGTCGGCGTGGAACTGACGCCGCTGGAAAAACTACCAATTGGCAAAGGCATTGTGAAG
CGTCGTGGCGAGAAACTGGCGATCCTTAACTTTGGTACGCTGATGCCAGAAGCGGCGAAA
GTCGCCGAATCGCTGAACGCCACGCTGGTCGATATGCGTTTTGTGAAACCGCTTGATGAA
GCGTTAATTCTGGAAATGGCCGCCAGCCATGAAGCGCTGGTCACCGTAGAAGAAAACGCC
ATTATGGGCGGCGCAGGCAGCGGCGTGAACGAAGTGCTGATGGCCCATCGTAAACCAGTA
CCCGTGCTGAACATTGGCCTGCCGGACTTCTTTATTCCGCAAGGAACTCAGGAAGAAATG
CGCGCCGAACTCGGCCTCGATGCCGCTGGTATGGAAGCCAAAATCAAGGCCTGGCTGGCA
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:620
Protein Molecular Weight:67616
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>1-deoxy-D-xylulose-5-phosphate synthase
MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVSRSSGHFASGLGTVELT
VALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKGGLHPFPWRGESEYDVLSV
GHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMAFEAMNHAGDIRPDMLVILND
NEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSGVPPIKELLKRTEEHIKGMVVPG
TLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQFLHIMTKKGRGYEPAEKDPITFHA
VPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAAKDNKLMAITPAMREGSGMVEFSRKFP
DRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIYSTFLQRAYDQVLHDVAIQKLPVLFAIDR
AGIVGADGQTHQGAFDLSYLRCIPEMVIMTPSDENECRQMLYTGYHYNDGPSAVRYPRGN
AVGVELTPLEKLPIGKGIVKRRGEKLAILNFGTLMPEAAKVAESLNATLVDMRFVKPLDE
ALILEMAASHEALVTVEENAIMGGAGSGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEM
RAELGLDAAGMEAKIKAWLA
References
External Links:
ResourceLink
Uniprot ID:P77488
Uniprot Name:DXS_ECOLI
GenBank Gene ID:AF035440
Genebank Protein ID:2665586
Ecogene ID:EG13612
Ecocyc:EG13612
ColiBase:b0420
Kegg Gene:b0420
EchoBASE ID:EB3378
CCDB:DXS_ECOLI
BacMap:16128405
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kuzuyama, T., Takagi, M., Takahashi, S., Seto, H. (2000). "Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp. Strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis." J Bacteriol 182:891-897. Pubmed: 10648511
  • Lois, L. M., Campos, N., Putra, S. R., Danielsen, K., Rohmer, M., Boronat, A. (1998). "Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis." Proc Natl Acad Sci U S A 95:2105-2110. Pubmed: 9482846
  • Sprenger, G. A., Schorken, U., Wiegert, T., Grolle, S., de Graaf, A. A., Taylor, S. V., Begley, T. P., Bringer-Meyer, S., Sahm, H. (1997). "Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol." Proc Natl Acad Sci U S A 94:12857-12862. Pubmed: 9371765