ECMDB

Identification

Name:

Cation efflux system protein cusB

Synonyms:

Not Available

Gene Name:

cusB

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in transmembrane transport

Specific Function:

Part of a cation efflux system that mediates resistance to copper and silver

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Two-component system ec02020

Transports:

Transport References:

Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831

Metabolites:

ECMDB ID	Name	View
ECMDB00657	Copper	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Component
cell part
membrane
Function
protein transporter activity
substrate-specific transporter activity
transporter activity
Process
establishment of localization
protein secretion
protein transport
transport

Gene Properties

Blattner:

b0574

Gene Orientation

Clockwise

Centisome Percentage:

12.86

Left Sequence End

596702

Right Sequence End

597925

Gene Sequence:

>1224 bp
ATGAAAAAAATCGCGCTTATTATCGGCAGCATGATCGCGGGCGGTATTATTTCTGCGGCA
GGTTTTACCTGGGTTGCAAAGGCGGAACCGCCTGCAGAAAAAACGTCGACCGCAGAACGT
AAAATCTTATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAA
TCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCT
GGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACG
CGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAG
TATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGGTGTATCCGCTTACCGTG
GGCGATAAAGTACAAAAGGGCACACCGCTTCTCGACCTGACCATTCCTGACTGGGTGGAA
GCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGC
ATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCC
ACGCAAAAAATCCAGACTCGCTTTACGCTCAAAGCGCCCATTGATGGCGTGATCACCGCG
TTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGT
ATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGAT
GCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAA
TGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTC
GACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCC
AGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGG
GTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCA
TCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGC
GGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCT
GAAAGTGCTACCCATGCGCATTGA

Protein Properties

Pfam Domain Function:

Not Available

Protein Residues:

407

Protein Molecular Weight:

44304

Protein Theoretical pI:

Signaling Regions:

1-28

Transmembrane Regions:

None

Protein Sequence:

>Cation efflux system protein cusB
MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILFWYDPMYPNTRFDKPGK
SPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQ
YAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEG
ILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQG
MDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEV
DNADEALKPGMNAWLQLNTASEPMLLIPSQALIDTGSEQRVITVDADGRFVPKRVAVFQA
SQGVTALRSGLAEGEKVVSSGLFLIDSEANISGALERMRSESATHAH

References

External Links:

Resource	Link
Uniprot ID:	P77239
Uniprot Name:	CUSB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062198
Ecogene ID:	EG14235
Ecocyc:	EG14235
ColiBase:	b0574
Kegg Gene:	b0574
EchoBASE ID:	EB3986
CCDB:	CUSB_ECOLI
BacMap:	16128557

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Franke, S., Grass, G., Nies, D. H. (2001). "The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions." Microbiology 147:965-972. Pubmed: 11283292
Franke, S., Grass, G., Rensing, C., Nies, D. H. (2003). "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli." J Bacteriol 185:3804-3812. Pubmed: 12813074
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Munson, G. P., Lam, D. L., Outten, F. W., O'Halloran, T. V. (2000). "Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12." J Bacteriol 182:5864-5871. Pubmed: 11004187
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
Outten, F. W., Huffman, D. L., Hale, J. A., O'Halloran, T. V. (2001). "The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli." J Biol Chem 276:30670-30677. Pubmed: 11399769

Cation efflux system protein cusB (P77239)