ECMDB: Lipid kinase yegS (P76407)

Identification

Name:

Lipid kinase yegS

Synonyms:

Not Available

Gene Name:

yegS

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in diacylglycerol kinase activity

Specific Function:

In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown

Cellular Location:

Cytoplasm

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

EcoCyc Reactions:

1.0an L-1-phosphatidyl-glycerol

1.0

→

1.0an L-1-phosphatidylglycerol-phosphate

1.0

1.0an L-1-phosphatidyl-glycerol + 1.0Adenosine triphosphate → 1.0an L-1-phosphatidylglycerol-phosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Function
binding
catalytic activity
cation binding
diacylglycerol kinase activity
ion binding
kinase activity
lipid kinase activity
metal ion binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
cell surface receptor linked signaling pathway
G-protein coupled receptor protein signaling pathway
signaling
signaling pathway

Gene Properties

Blattner:

b2086

Gene Orientation

Clockwise

Centisome Percentage:

46.70

Left Sequence End

2166736

Right Sequence End

2167635

Gene Sequence:

>900 bp
ATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGC
TTCACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGT
CGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCA
ACTGAAGCGGGCGAGCATCTTTTGTCTGTTCTTGGCCCTATGTTGCATGACATAGATTCA
GCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACT
GTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCAT
CCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGT
TTTGATGCAGGCGTCCGTCTGGGTGGGGAGATGGATAAAGATATGATCGCCATTCGAATC
GGGCCAGATATACCAATGGCTATTGTTGGCTCACCGGATTATTTTTCTCGCCGAAGTGTT
CCAACGTCAGTGTCACAATTAATAGATCATCAGGCAATTAATTTGTATCTTCCCACATCG
GGTACAGCAAATCGCTGGAGATTAATACGCGGTGGACGTGAAGTTCGTGTTCGCATGGAA
GGTCAGCTTTTACTGAATACGATAGACCTGATCATTGATGCTGCAATTGATGGGCATGGA
TTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAACTGATACGT
GTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTATCCACACCGTCGA
CATGCTGGCTCGGCATTCTCATTATTTATAGATAGGCTGAAGTATAAAGGTGCTGTTTAG

Protein Properties

Pfam Domain Function:

DAGK_cat (PF00781 )

Protein Residues:

299

Protein Molecular Weight:

32038

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Lipid kinase yegS
MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKGDAARYVEEARKFGVAT
VIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATSVGIPEALDKALKLAIAGD
AIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALGSVSYIIHGLMRMDTLQPDRC
EIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALINDGLLQLRIFTGDEILPALVSTLK
SDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQNFHIEILPAALRCRLPPDCPLLR

References

External Links:

Resource	Link
Uniprot ID:	P76407
Uniprot Name:	YEGS_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1742185
Ecogene ID:	EG14367
Ecocyc:	EG14367
ColiBase:	b2086
Kegg Gene:	b2086
EchoBASE ID:	EB4111
CCDB:	YEGS_ECOLI
BacMap:	16130026

General Reference:

Bakali, H. M., Herman, M. D., Johnson, K. A., Kelly, A. A., Wieslander, A., Hallberg, B. M., Nordlund, P. (2007). "Crystal structure of YegS, a homologue to the mammalian diacylglycerol kinases, reveals a novel regulatory metal binding site." J Biol Chem 282:19644-19652. Pubmed: 17351295
Bakali, M. A., Nordlund, P., Hallberg, B. M. (2006). "Expression, purification, crystallization and preliminary diffraction studies of the mammalian DAG kinase homologue YegS from Escherichia coli." Acta Crystallogr Sect F Struct Biol Cryst Commun 62:295-297. Pubmed: 16511327
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040