ECMDB

Identification

Name:

Protein rutD

Synonyms:

Pyrimidine utilization protein D

Gene Name:

rutD

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Specific Function:

Makes part of the rut operon, which is required for the utilization of pyrimidines as sole nitrogen source

Cellular Location:

Not Available

SMPDB Pathways:

Pyrimidine metabolism PW000942
Uracil degradation III PW002026

KEGG Pathways:

Pyrimidine metabolism ec00240

KEGG Reactions:

1.0

↔

1.0

1.03-Aminoacrylate + 1.0Water ↔ 1.0Malonic semialdehyde + 1.0Ammonia
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.02-Aminoacrylic acid + 1.0Water + 1.0Hydrogen ion → 1.0Malonic semialdehyde + 1.0Ammonium
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.03-Aminoacrylate + 1.0Water ↔ 1.0Malonic semialdehyde + 1.0Ammonia
ReactionCard

1.0

→

1.0

1.03-Aminoacrylate + 1.0Water → 1.0Malonic semialdehyde + 1.0Ammonia
ReactionCard

Complex Reactions:

1.0

→

1.0

1.03-Aminoacrylate + 1.0Hydrogen ion + 1.0Water → 1.0Malonic semialdehyde + 1.0Ammonium
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB03609	2-Aminoacrylic acid	MetaboCard
ECMDB21167	3-Aminoacrylate	MetaboCard
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB03015	Malonic semialdehyde	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b1009

Gene Orientation

Counterclockwise

Centisome Percentage:

23.07

Left Sequence End

1070188

Right Sequence End

1070988

Gene Sequence:

>801 bp
GTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTA
CCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACC
GAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTG
TCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACA
ACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCGCCGATCAGGTACCCGCT
TTCGTGCTGCCACAACATCCTTCTGGCGTAATGACCCTTTCGGCGGCACATCCAGTGTAT
TGCTGCCCGTTACGTGAACGAGAACCCATTTTGCGTCTGGCGAAGTCGGCACTGGTGACG
GTCTATCCATTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTC
AGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCAC
CACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAAC
GCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAG
CGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGT
GAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGT
CCCGGCAAGCCTGATAAATAA

Protein Properties

Pfam Domain Function:

Abhydrolase_1 (PF00561 )

Protein Residues:

266

Protein Molecular Weight:

28898

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Protein rutD
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAED
YSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAH
TRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNLLR
RLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSSELHAALPDSQKMVMPYGGHACN
VTDPETFNALLLNGLASLLHHREAAL

References

External Links:

Resource	Link
Uniprot ID:	P75895
Uniprot Name:	RUTD_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674383
Ecogene ID:	EG13856
Ecocyc:	EG13856
ColiBase:	b1009
Kegg Gene:	b1009
EchoBASE ID:	EB3616
CCDB:	RUTD_ECOLI
BacMap:	16128975

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Loh, K. D., Gyaneshwar, P., Markenscoff Papadimitriou, E., Fong, R., Kim, K. S., Parales, R., Zhou, Z., Inwood, W., Kustu, S. (2006). "A previously undescribed pathway for pyrimidine catabolism." Proc Natl Acad Sci U S A 103:5114-5119. Pubmed: 16540542
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232

Protein rutD (P75895)