Putative NADH dehydrogenase/NAD(P)H nitroreductase rutE (P75894)

Identification
Name:Putative NADH dehydrogenase/NAD(P)H nitroreductase rutE
Synonyms:
  • Pyrimidine utilization protein E
Gene Name:rutE
Enzyme Class:Not Available
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Makes part of the rut operon, which is required for the utilization of pyrimidines as sole nitrogen source
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Malonic semialdehyde + 1.0NADPH → 1.03-Hydroxypropanoate + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Hydroxypropanoate + 1.0NADP ↔ 1.0Malonic semialdehyde + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Malonic semialdehyde + 1.0NADPH → 1.03-Hydroxypropanoate + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Hydroxypropanoate + 1.0NADP ← 1.0Malonic semialdehyde + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Hydroxypropanoate + 1.0NADP → 1.0Malonic semialdehyde + 1.0NADPH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211683-HydroxypropanoateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB03015Malonic semialdehydeMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
catalytic activity
oxidoreductase activity
Gene Properties
Blattner:b1008
Gene OrientationCounterclockwise
Centisome Percentage:23.05
Left Sequence End1069588
Right Sequence End1070178
Gene Sequence:
>591 bp
ATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCA
CATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTC
GATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGACTGGAAG
AATATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACAT
GACACGTTAGTGGTGGTTTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGA
TATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTC
AATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGACAAAGAATCATTTGTCCAGATGGGC
TGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGAT
GCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGC
TATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAA
Protein Properties
Pfam Domain Function:
Protein Residues:196
Protein Molecular Weight:21570
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Putative NADH dehydrogenase/NAD(P)H nitroreductase rutE
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFTRTA
EGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEET
AFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFTGSTLKSNLLINIGYGDSSKL
YARLPRLSFEEACGLL
References
External Links:
ResourceLink
Uniprot ID:P75894
Uniprot Name:RUTE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062477
Ecogene ID:EG13855
Ecocyc:EG13855
ColiBase:b1008
Kegg Gene:b1008
EchoBASE ID:EB3615
CCDB:RUTE_ECOLI
BacMap:16128974
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Loh, K. D., Gyaneshwar, P., Markenscoff Papadimitriou, E., Fong, R., Kim, K. S., Parales, R., Zhou, Z., Inwood, W., Kustu, S. (2006). "A previously undescribed pathway for pyrimidine catabolism." Proc Natl Acad Sci U S A 103:5114-5119. Pubmed: 16540542
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232