ECMDB: Putative NADH dehydrogenase/NAD(P)H nitroreductase rutE (P75894)

Identification

Name:

Putative NADH dehydrogenase/NAD(P)H nitroreductase rutE

Synonyms:

Pyrimidine utilization protein E

Gene Name:

rutE

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in oxidoreductase activity

Specific Function:

Makes part of the rut operon, which is required for the utilization of pyrimidines as sole nitrogen source

Cellular Location:

Not Available

SMPDB Pathways:

Pyrimidine metabolism PW000942
Uracil degradation III PW002026

KEGG Pathways:

Pyrimidine metabolism ec00240

KEGG Reactions:

1.0

→

1.0

1.0Hydrogen ion + 1.0Malonic semialdehyde + 1.0NADPH → 1.03-Hydroxypropanoate + 1.0NADP
ReactionCard

1.0

↔

1.0

1.03-Hydroxypropanoate + 1.0NADP ↔ 1.0Malonic semialdehyde + 1.0NADPH + 1.0Hydrogen ion
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0Hydrogen ion + 1.0Malonic semialdehyde + 1.0NADPH → 1.03-Hydroxypropanoate + 1.0NADP
ReactionCard

1.0

←

1.0

1.03-Hydroxypropanoate + 1.0NADP ← 1.0Malonic semialdehyde + 1.0NADPH + 1.0Hydrogen ion
ReactionCard

Complex Reactions:

1.0

→

1.0

1.03-Hydroxypropanoate + 1.0NADP → 1.0Malonic semialdehyde + 1.0NADPH
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21168	3-Hydroxypropanoate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB03015	Malonic semialdehyde	MetaboCard
ECMDB00217	NADP	MetaboCard
ECMDB04111	NADPH	MetaboCard

GO Classification:

Function
catalytic activity
oxidoreductase activity

Gene Properties

Blattner:

b1008

Gene Orientation

Counterclockwise

Centisome Percentage:

23.05

Left Sequence End

1069588

Right Sequence End

1070178

Gene Sequence:

>591 bp
ATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCA
CATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTC
GATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGACTGGAAG
AATATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACAT
GACACGTTAGTGGTGGTTTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGA
TATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATGGGCACAAATTACCATTC
AATAAAGTTCGTTGGGTGGCGCTGGTTGGAGGAGACAAAGAATCATTTGTCCAGATGGGC
TGGGAAAAAAATATAAGCGATTATTTAAAAAATATGTGCAGTTATCTTGGTATTGAAGAT
GCCGATGTCACTTTCTTGTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGC
TATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAA

Protein Properties

Pfam Domain Function:

Nitroreductase (PF00881 )

Protein Residues:

196

Protein Molecular Weight:

21570

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Putative NADH dehydrogenase/NAD(P)H nitroreductase rutE
MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFTRTA
EGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEET
AFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFTGSTLKSNLLINIGYGDSSKL
YARLPRLSFEEACGLL

References

External Links:

Resource	Link
Uniprot ID:	P75894
Uniprot Name:	RUTE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062477
Ecogene ID:	EG13855
Ecocyc:	EG13855
ColiBase:	b1008
Kegg Gene:	b1008
EchoBASE ID:	EB3615
CCDB:	RUTE_ECOLI
BacMap:	16128974

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Loh, K. D., Gyaneshwar, P., Markenscoff Papadimitriou, E., Fong, R., Kim, K. S., Parales, R., Zhou, Z., Inwood, W., Kustu, S. (2006). "A previously undescribed pathway for pyrimidine catabolism." Proc Natl Acad Sci U S A 103:5114-5119. Pubmed: 16540542
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232