Identification
Name:Putative pyruvate formate-lyase 3-activating enzyme
Synonyms:
  • Formate-C-acetyltransferase-activating enzyme 3
  • PFL-activating enzyme 3
Gene Name:ybiY
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB211745'-DeoxyadenosineMetaboCard
ECMDB00696L-MethionineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
4 iron, 4 sulfur cluster binding
binding
catalytic activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
Process
alcohol metabolic process
cellular metabolic process
glucose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
oxidation reduction
oxygen and reactive oxygen species metabolic process
small molecule metabolic process
Gene Properties
Blattner:b0824
Gene OrientationCounterclockwise
Centisome Percentage:18.58
Left Sequence End861835
Right Sequence End862734
Gene Sequence:
>927 bp
ATGCTTGAACGAAATAGAGAGGCAACTATGATTTTCAATATTCAGCGCTACTCGACCCAT
GATGGCCCCGGTATCCGCACGGTCGTATTTCTTAAAGGCTGTTCGCTGGGCTGCCGCTGG
TGTCAGAACCCGGAAAGCCGCGCCCGCACGCAGGATCTGCTGTATGACGCACGACTGTGT
CTGGAAGGCTGCGAGCTGTGCGCTAAGGCCGCGCCGGAAGTGATTGAGCGCGCGCTGAAT
GGTTTGCTTATTCATCGGGAAAAGTTAACCCCGGAGCATCTGACGGCGTTAACCGACTGC
TGTCCGACACAGGCATTAACCGTGTGTGGTGAAGTGAAAAGCGTTGAGGAGATCATGACG
ACCGTTCTGCGCGATAAACCGTTTTACGATCGCAGCGGCGGCGGTTTAACGCTTTCGGGT
GGTGAGCCCTTTATGCAGCCGGAAATGGCGATGGCGCTACTGCAAGCCAGCCACGAGGCA
GGCATTCATACTGCGGTAGAAACCTGTCTGCATGTGCCGTGGAAATATATCGCCCCTTCT
CTGCCCTATATCGATCTGTTTCTTGCCGATTTAAAACACGTTGCCGACGCGCCGTTTAAA
CAGTGGACCGACGGTAACGCCGCCAGAGTGCTGGATAACCTGAAAAAACTCGCCGCAGCG
GGCAAAAAAATCATTATCCGCGTGCCGCTGATTCAGGGCTTTAATGCCGACGAAACCTCT
GTAAAAGCCATTACCGATTTTGCCGCCGACGAGCTGCACGTTGGCGAAATTCATTTTCTG
CCCTACCACACGCTGGGCATCAACAAATATCACTTACTTAATCTGCCCTATGACGCCCCG
GAAAAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCCAGCAGTATGCCTGCCAGAAA
GGGTTAACCGCGACCTTACGAGGATAA
Protein Properties
Pfam Domain Function:
Protein Residues:299
Protein Molecular Weight:33038
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Putative pyruvate formate-lyase 3-activating enzyme
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAK
AAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMTTVLRDKPFY
DRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETCLHVPWKYIAPSLPYIDLFLA
DLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETSVKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
References
External Links:
ResourceLink
Uniprot ID:P75794
Uniprot Name:PFLE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062398
Ecogene ID:EG13470
Ecocyc:EG13470
ColiBase:b0824
Kegg Gene:b0824
EchoBASE ID:EB3243
CCDB:PFLE_ECOLI
BacMap:226524704
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232