Galactitol-specific phosphotransferase enzyme IIA component (P69828)

Identification
Name:Galactitol-specific phosphotransferase enzyme IIA component
Synonyms:
  • EIIA-Gat
  • PTS system galactitol-specific EIIA component
Gene Name:gatA
Enzyme Class:
Biological Properties
General Function:Involved in transporter activity
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in galactitol transport
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Protein N(pi)-phospho-L-histidine ↔ 1.0Thumb+1.0Protein histidine
1.0Galactitol + 1.0Protein N(pi)-phospho-L-histidine ↔ 1.0Galactitol 1-phosphate + 1.0Protein histidine
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0HPr - phosphorylated → 1.0Galactose 1-phosphate+1.0HPr+1.0Thumb
1.0Galactitol + 1.0HPr - phosphorylated → 1.0Galactose 1-phosphate + 1.0HPr + 1.0Galactose 1-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Galactitol → 1.0Galactitol 1-phosphate + 1.0Pyruvic acid
ReactionCard
Complex Reactions:
1.0Protein EIIA N(pi)-phospho-L-histidine+1.0protein EIIB → 1.0protein EIIA+1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00107GalactitolMetaboCard
ECMDB20149Galactitol 1-phosphateMetaboCard
ECMDB04011Galactose 1-phosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Function
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b2094
Gene OrientationCounterclockwise
Centisome Percentage:46.83
Left Sequence End2172619
Right Sequence End2173071
Gene Sequence:
>453 bp
ATGAATAACATACAAATAAGAAACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATT
TTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCC
TGGGCGCAGATTGACGAATCTCGCTGGAAGGAGAAACTCGCGAAATCACAAGTGTGGGTT
GCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATG
TTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTG
ATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAA
CGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACT
AATTTTTATATGCGATATAAACCGCAACATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:150
Protein Molecular Weight:16907
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Galactitol-specific phosphotransferase enzyme IIA component
MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAI
PHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKL
QQPDIVETLITLPETQLKEYFTKYVLDSDE
References
External Links:
ResourceLink
Uniprot ID:P69828
Uniprot Name:PTKA_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786428
Ecogene ID:EG12414
Ecocyc:EG12414
ColiBase:b2094
Kegg Gene:b2094
EchoBASE ID:EB2313
CCDB:PTKA_ECOLI
BacMap:16130032
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nobelmann, B., Lengeler, J. W. (1995). "Sequence of the gat operon for galactitol utilization from a wild-type strain EC3132 of Escherichia coli." Biochim Biophys Acta 1262:69-72. Pubmed: 7772602