| Identification |
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| Name: | Ascorbate-specific phosphotransferase enzyme IIB component |
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| Synonyms: | - Ascorbate-specific PTS system EIIB component
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| Gene Name: | ulaB |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in sugar:hydrogen symporter activity |
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| Specific Function: | The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in ascorbate transport |
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| Cellular Location: | Cytoplasm (Potential) |
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| SMPDB Pathways: | |
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| KEGG Pathways: | - Amino sugar and nucleotide sugar metabolism ec00520
- Ascorbate and aldarate metabolism ec00053
- Fructose and mannose metabolism ec00051
- Galactose metabolism ec00052
- Glycolysis / Gluconeogenesis ec00010
- Metabolic pathways eco01100
- Microbial metabolism in diverse environments ec01120
- Pentose phosphate pathway ec00030
- Starch and sucrose metabolism ec00500
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| KEGG Reactions: | |
1.0Protein N(pi)-phospho-L-histidine | + | 1.0 | ↔ | 1.0Protein histidine | + | 1.0 |
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1.0Protein N(pi)-phospho-L-histidine | + | 1.0Sugar | ↔ | 1.0Protein histidine | + | 1.0 |
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| SMPDB Reactions: | |
1.0Ascorbic acid | + | 1.0HPr - phosphorylated | + | 1.0 | → | 1.0L-ascorbate 6-phosphate | + | 1.0HPr | + | 1.0 |
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| EcoCyc Reactions: | |
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| Complex Reactions: | |
1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine | + | 1.0 | → | 1.0protein EIIB | + | 1.0sugar phosphate |
| 1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine + 1.0 Sucrose → 1.0protein EIIB + 1.0sugar phosphate ReactionCard |
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| Metabolites: | |
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| GO Classification: | | Function |
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| carbohydrate transmembrane transporter activity | | cation transmembrane transporter activity | | cation:sugar symporter activity | | ion transmembrane transporter activity | | protein-N(PI)-phosphohistidine-sugar phosphotransferase activity | | solute:cation symporter activity | | substrate-specific transmembrane transporter activity | | sugar:hydrogen symporter activity | | transmembrane transporter activity | | transporter activity | | Process |
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| carbohydrate transport | | establishment of localization | | phosphoenolpyruvate-dependent sugar phosphotransferase system | | transport |
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| Gene Properties |
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| Blattner: | b4194 |
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| Gene Orientation | Clockwise |
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| Centisome Percentage: | 95.25 |
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| Left Sequence End | 4419416 |
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| Right Sequence End | 4419721 |
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| Gene Sequence: | >306 bp
ATGTCATTACAAGTATCGCACTACAACATGCTTAGGGCAAGTCACGAAGGAAGTCAGAAA
GTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCC
ATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACA
GACACCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATT
TTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGACCTGT
CGATAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 101 |
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| Protein Molecular Weight: | 10896 |
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| Protein Theoretical pI: | 7 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >Ascorbate-specific phosphotransferase enzyme IIB component
MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEYKSELSGADIIIASTHI
AGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
- Campos, E., Aguilar, J., Baldoma, L., Badia, J. (2002). "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli." J Bacteriol 184:6065-6068. Pubmed: 12374842
- Campos, E., Baldoma, L., Aguilar, J., Badia, J. (2004). "Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in LaAscorbate dissimilation in Escherichia coli." J Bacteriol 186:1720-1728. Pubmed: 14996803
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Zhang, Z., Aboulwafa, M., Smith, M. H., Saier, M. H. Jr (2003). "The ascorbate transporter of Escherichia coli." J Bacteriol 185:2243-2250. Pubmed: 12644495
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