Ascorbate-specific phosphotransferase enzyme IIA component (P69820)

Identification
Name:Ascorbate-specific phosphotransferase enzyme IIA component
Synonyms:
  • PTS system ascorbate-specific EIIA component
Gene Name:ulaC
Enzyme Class:
Biological Properties
General Function:Involved in transporter activity
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in ascorbate transport
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Protein N(pi)-phospho-L-histidine+1.0Thumb ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Ascorbic acid ↔ 1.0Protein histidine + 1.0L-Ascorbate 6-phosphate
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
SMPDB Reactions:
1.0Ascorbic acid+1.0HPr - phosphorylated+1.0Thumb → 1.0L-ascorbate 6-phosphate+1.0HPr+1.0Thumb
1.0Ascorbic acid + 1.0HPr - phosphorylated + 1.0Ascorbic acid → 1.0L-ascorbate 6-phosphate + 1.0HPr + 1.0L-Ascorbate 6-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Ascorbic acid → 1.0L-Ascorbate 6-phosphate + 1.0Pyruvic acid
ReactionCard
Complex Reactions:
1.0Protein EIIA N(pi)-phospho-L-histidine+1.0protein EIIB → 1.0protein EIIA+1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00044Ascorbic acidMetaboCard
ECMDB20164L-Ascorbate 6-phosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Function
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b4195
Gene OrientationClockwise
Centisome Percentage:95.26
Left Sequence End4419731
Right Sequence End4420195
Gene Sequence:
>465 bp
ATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTA
TTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTT
GCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCGCTGGAT
ACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGC
CTGAAAGATGAAGCTACCCGCTCGCGGCTGAGTGAGTATTTGCCGGAAGTCCGTAGTCGC
TTGCTGTTACTGTTTTCGCGTCAGGATGCTGCCGTACTGGCGACAGAAGAAGGCAAGAAA
AACCTGATTGCCGAGATTAAAACCACACTTTCCACCCCGCTTGTTGCCGGGCAACCGAAA
CAGGATGTCACCGACGTGCTGTATACCGCTTTTATTCTGCGATAA
Protein Properties
Pfam Domain Function:
Protein Residues:154
Protein Molecular Weight:17237
Protein Theoretical pI:4
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ascorbate-specific phosphotransferase enzyme IIA component
MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVI
APGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQ
IVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA
References
External Links:
ResourceLink
Uniprot ID:P69820
Uniprot Name:ULAC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736610
Ecogene ID:EG12495
Ecocyc:EG12495
ColiBase:b4195
Kegg Gene:b4195
EchoBASE ID:EB2388
CCDB:ULAC_ECOLI
BacMap:16132017
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Campos, E., Aguilar, J., Baldoma, L., Badia, J. (2002). "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli." J Bacteriol 184:6065-6068. Pubmed: 12374842
  • Campos, E., Baldoma, L., Aguilar, J., Badia, J. (2004). "Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in LaAscorbate dissimilation in Escherichia coli." J Bacteriol 186:1720-1728. Pubmed: 14996803
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Zhang, Z., Aboulwafa, M., Smith, M. H., Saier, M. H. Jr (2003). "The ascorbate transporter of Escherichia coli." J Bacteriol 185:2243-2250. Pubmed: 12644495