Ferrous-iron efflux pump fieF (P69380)

Identification
Name:Ferrous-iron efflux pump fieF
Synonyms:Not Available
Gene Name:fieF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in cation transmembrane transporter activity
Specific Function:Iron-efflux transporter responsible for iron detoxification. Also able to transport Zn(2+) in a proton- dependent manner
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21194CadmiumMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00692IronMetaboCard
ECMDB01333ManganeseMetaboCard
ECMDB21391NickelMetaboCard
ECMDB01303ZincMetaboCard
GO Classification:
Component
cell part
membrane
Function
cation transmembrane transporter activity
ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
transmembrane transport
transport
Gene Properties
Blattner:b3915
Gene OrientationClockwise
Centisome Percentage:88.47
Left Sequence End4104492
Right Sequence End4105394
Gene Sequence:
>903 bp
ATGGATGCACCGAGTACCACACCGCATGACGCGGTATTTAAACAATTTTTAATGCATGCG
GAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGAC
CTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGC
ACGGACGTGCTCTATTCCGTGCAAATGCAGGGCAATCCCGGTTATCTGCATGTTGTGATT
GAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCC
GCCATGCACCGGCATCTGGAGGCTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTG
TTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTAC
TCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATC
ACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAA
CATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAA
GGGTACACTAGCGGAAGTCAGTTAGTTGCCATGCAAAACTATATGCTGCAACGCGGTCAT
ACTGAACAAGCGGATTTGTTTTACGGTGTGTTGAGAGACAGGGAAACGGGAGGGGAGTCT
ATGATGACGCTGGCGCAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGA
AGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAA
GACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATT
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:300
Protein Molecular Weight:32927
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 12-34
  • 38-60
  • 80-102
  • 117-139
  • 158-175
  • 180-199
Protein Sequence:
>Ferrous-iron efflux pump fieF
MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALVDSLVDIGASLTNLLVV
RYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGIQHLISPTPMTDPGVGVIV
TIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMMNGAILLALGLSWYGWHRADA
LFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEIIDIVTSWPGVSGAHDLRTRQSG
PTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFPGSDVIIHQDPCSVVPREGKRSMLS
References
External Links:
ResourceLink
Uniprot ID:P69380
Uniprot Name:FIEF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674347
Ecogene ID:EG11873
Ecocyc:EG11873
ColiBase:b3915
Kegg Gene:b3915
EchoBASE ID:EB1819
CCDB:FIEF_ECOLI
BacMap:16131753
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Grass, G., Otto, M., Fricke, B., Haney, C. J., Rensing, C., Nies, D. H., Munkelt, D. (2005). "FieF (YiiP) from Escherichia coli mediates decreased cellular accumulation of iron and relieves iron stress." Arch Microbiol 183:9-18. Pubmed: 15549269
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
  • Wei, Y., Li, H., Fu, D. (2004). "Oligomeric state of the Escherichia coli metal transporter YiiP." J Biol Chem 279:39251-39259. Pubmed: 15258151