N-acetylmuramoyl-L-alanine amidase AmiC (P63883)

Identification
Name:N-acetylmuramoyl-L-alanine amidase AmiC
Synonyms:Not Available
Gene Name:amiC
Enzyme Class:
Biological Properties
General Function:Involved in N-acetylmuramoyl-L-alanine amidase activity
Specific Function:Cell-wall hydrolase probably involved in cell-wall hydrolysis, septation or recycling
Cellular Location:Secreted (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:
  • Cationic antimicrobial peptide (CAMP) resistance eco01503
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0N-Acetylmuramoyl-Ala + 1.0Water ↔ 1.0N-Acetyl-D-muramoate + 1.0L-Alanine
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + 1.0Water → 1.0L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + 1.0Water → 1.0L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00161L-AlanineMetaboCard
ECMDB21240L-alanine-D-glutamate-meso-2,6-diaminoheptanedioateMetaboCard
ECMDB21239L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanineMetaboCard
ECMDB21248N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acidMetaboCard
ECMDB21249N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB21250N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
ECMDB20176N-Acetyl-D-muramoateMetaboCard
ECMDB20178N-Acetylmuramoyl-AlaMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b2817
Gene OrientationCounterclockwise
Centisome Percentage:63.49
Left Sequence End2945779
Right Sequence End2947032
Gene Sequence:
>1254 bp
ATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCC
AGCCGCGAAAAAAATCGCGTGCTGGAAAAAATCGCCGATGAACTGGAAGCACAAAGCGAA
ATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAA
GCGATGCTTGACCGTCTGGCACTGACGCCCGCACGGCTGAAAGGCATTGCCGACGATGTA
CGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTACTGGAC
AGCGGCCTGCGTCTTGAGCGTCGTCGCGTACCGCTGGGGGTTATTGGCGTGATTTATGAA
GCGCGCCCGAACGTGACGGTTGATGTCGCTTCGCTGTGCCTGAAAACCGGTAATGCGGTG
ATCCTGCGCGGTGGCAAAGAAACGTGTCGCACTAACGCTGCAACGGTGGCGGTGATTCAG
GACGCCCTGAAATCCTGCGGCTTACCGGCGGGTGCCGTGCAGGCGATTGATAATCCTGAC
CGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGT
GGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGT
GGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAA
GTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTG
GTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGC
GGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAG
GTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTC
AAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACAC
TCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGAT
TCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTG
GGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGGGGCTGGAA
GCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:417
Protein Molecular Weight:45634
Protein Theoretical pI:10
Signaling Regions:
  • 1-31
Transmembrane Regions:
  • None
Protein Sequence:
>N-acetylmuramoyl-L-alanine amidase AmiC
MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRVTVESNRQLKY
KQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELKQN
VKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQPG
KAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTRNE
DIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQN
ASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFA
VLKAPDIPSILVETAFISNVEEERKLKTATFQQEVAESILAGIKAYFADGATLARRG
References
External Links:
ResourceLink
Uniprot ID:P63883
Uniprot Name:AMIC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674395
Ecogene ID:EG13086
Ecocyc:EG13086
ColiBase:b2817
Kegg Gene:b2817
EchoBASE ID:EB2895
CCDB:AMIC_ECOLI
BacMap:90111492
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Tullman-Ercek, D., DeLisa, M. P., Kawarasaki, Y., Iranpour, P., Ribnicky, B., Palmer, T., Georgiou, G. (2007). "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." J Biol Chem 282:8309-8316. Pubmed: 17218314