Identification
Name:Cytoplasmic trehalase
Synonyms:
  • Alpha,alpha-trehalase
  • Alpha,alpha-trehalose glucohydrolase
Gene Name:treF
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Secondary metabolites: Trehalose Biosynthesis and Metabolism PW000968
  • Starch and sucrose metabolism PW000941
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb2.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Beta-D-Glucose+1.0Thumb
1.0α,α-trehalose + 1.0Water → 1.0alpha-D-Glucose + 1.0Beta-D-Glucose + 1.0b-D-Glucose
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB00975TrehaloseMetaboCard
ECMDB00494WaterMetaboCard
ECMDB24256α,α-trehaloseMetaboCard
GO Classification:
Function
alpha,alpha-trehalase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
trehalase activity
Process
carbohydrate metabolic process
disaccharide metabolic process
metabolic process
oligosaccharide metabolic process
primary metabolic process
trehalose metabolic process
Gene Properties
Blattner:b3519
Gene OrientationClockwise
Centisome Percentage:79.05
Left Sequence End3667615
Right Sequence End3669264
Gene Sequence:
>1650 bp
ATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTC
TGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAA
CCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTC
GAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGAC
TGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAT
CGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCC
AGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCG
GTGCTAACCCGCGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAG
TCATATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATTGGGATTCCTATTTCACC
ATGCTGGGGCTGGCGGAAAGTGGTCGGGAAGATTTGCTGAAATGCATGGCCGATAACTTC
GCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGC
CGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGTTGTTTGAAGAAGATGGTGTACGC
GGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCA
GAATCGTTAATCCCTAATCAGGCCTATCGCCATGTTGTGCGGATGCCGGACGGATCGCTG
CTCAACCGTTACTGGGACGATCGCGACACGCCGCGTGACGAATCCTGGCTTGAGGACGTT
GAAACCGCGAAACATTCTGGTCGCCCGCCCAACGAGGTGTACCGCGATTTACGCGCGGGG
GCGGCCTCCGGTTGGGATTACTCTTCCCGTTGGCTGCGTGATACTGGTCGTCTGGCGAGC
ATTCGTACCACCCAGTTCATCCCCATCGATCTGAATGCCTTCCTGTTTAAACTGGAGAGC
GCCATCGCCAACATCTCGGCGCTGAAAGGCGAGAAAGAGACAGAAGCACTGTTCCGCCAG
AAAGCCAGTGCCCGTCGCGATGCGGTAAACCGTTACCTCTGGGATGATGAAAACGGCATC
TACCGCGATTACGACTGGCGACGCGAACAACTGGCGCTGTTTTCCGCTGCCGCCATTGTG
CCACTCTATGTCGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGC
AGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGG
GATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTAC
GGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTC
TATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAA
GGCGGCGGTGGCGAGTATCCGTTGCAGGATGGGTTTGGCTGGACTAACGGTGTGGTACGC
CGTTTAATTGGTTTGTACGGCGAACCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:549
Protein Molecular Weight:63696
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Cytoplasmic trehalase
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
SEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
RLIGLYGEP
References
External Links:
ResourceLink
Uniprot ID:P62601
Uniprot Name:TREF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676525
Ecogene ID:EG12245
Ecocyc:EG12245
ColiBase:b3519
Kegg Gene:b3519
EchoBASE ID:EB2156
CCDB:TREF_ECOLI
BacMap:16131391
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Horlacher, R., Uhland, K., Klein, W., Ehrmann, M., Boos, W. (1996). "Characterization of a cytoplasmic trehalase of Escherichia coli." J Bacteriol 178:6250-6257. Pubmed: 8892826
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Uhland, K., Mondigler, M., Spiess, C., Prinz, W., Ehrmann, M. (2000). "Determinants of translocation and folding of TreF, a trehalase of Escherichia coli." J Biol Chem 275:23439-23445. Pubmed: 10816581