Identification |
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Name: | Agmatinase |
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Synonyms: | - Agmatine ureohydrolase
- AUH
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Gene Name: | speB |
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Enzyme Class: | |
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Biological Properties |
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General Function: | Involved in agmatinase activity |
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Specific Function: | Catalyzes the formation of putrescine from agmatine |
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Cellular Location: | Cytoplasmic |
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SMPDB Pathways: | |
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KEGG Pathways: | |
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KEGG Reactions: | |
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EcoCyc Reactions: | |
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Metabolites: | |
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GO Classification: | Function |
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agmatinase activity | binding | catalytic activity | cation binding | hydrolase activity | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines | ion binding | metal ion binding | Process |
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cellular amino acid and derivative metabolic process | cellular amino acid derivative metabolic process | cellular biogenic amine metabolic process | cellular metabolic process | metabolic process | polyamine biosynthetic process | polyamine metabolic process |
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Gene Properties |
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Blattner: | b2937 |
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Gene Orientation | Counterclockwise |
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Centisome Percentage: | 66.40 |
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Left Sequence End | 3080899 |
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Right Sequence End | 3081819 |
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Gene Sequence: | >921 bp
ATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTG
AATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTC
GACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATC
GCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGC
TTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGC
AAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCA
GAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCG
GTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGAA
AATGCTCTACAAGATGCATTAAGATTGGCATTTCAGCACGATGAAGAAGTATTGATTGAA
AAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCA
ATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAG
ACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGAGGCCAATTTGCAGGCATTA
GTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTG
GACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCAC
AGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGA
ATTCTGGAACTGGCGGACTAA |
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Protein Properties |
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Pfam Domain Function: | |
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Protein Residues: | 306 |
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Protein Molecular Weight: | 33557 |
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Protein Theoretical pI: | 5 |
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Signaling Regions: | |
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Transmembrane Regions: | |
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Protein Sequence: | >Agmatinase
MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIR
QVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRML
SFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEFDHGTMFYTAPKEGLIDPNH
SVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMPVYLTFDIDCLDPAFAP
GTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQ
AAKKGE |
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References |
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External Links: | |
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General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Carvajal, N., Lopez, V., Salas, M., Uribe, E., Herrera, P., Cerpa, J. (1999). "Manganese is essential for catalytic activity of Escherichia coli agmatinase." Biochem Biophys Res Commun 258:808-811. Pubmed: 10329468
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Moore, R. C., Boyle, S. M. (1990). "Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli." J Bacteriol 172:4631-4640. Pubmed: 2198270
- Szumanski, M. B., Boyle, S. M. (1990). "Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli." J Bacteriol 172:538-547. Pubmed: 2153656
- Szumanski, M. B., Boyle, S. M. (1992). "Influence of cyclic AMP, agmatine, and a novel protein encoded by a flanking gene on speB (agmatine ureohydrolase) in Escherichia coli." J Bacteriol 174:758-764. Pubmed: 1310091
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