Agmatinase (P60651)

Identification
Name:Agmatinase
Synonyms:
  • Agmatine ureohydrolase
  • AUH
Gene Name:speB
Enzyme Class:
Biological Properties
General Function:Involved in agmatinase activity
Specific Function:Catalyzes the formation of putrescine from agmatine
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Agmatine + 1.0Water ↔ 1.0Putrescine + 1.0Urea + 1.0Ethylenediamine
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Agmatine + 1.0Water ↔ 1.0Putrescine + 1.0Urea
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Agmatine + 1.0Water ↔ 1.0Putrescine + 1.0Urea + 1.0Ethylenediamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01432AgmatineMetaboCard
ECMDB20274EthylenediamineMetaboCard
ECMDB01414PutrescineMetaboCard
ECMDB04172UreaMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
agmatinase activity
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines
ion binding
metal ion binding
Process
cellular amino acid and derivative metabolic process
cellular amino acid derivative metabolic process
cellular biogenic amine metabolic process
cellular metabolic process
metabolic process
polyamine biosynthetic process
polyamine metabolic process
Gene Properties
Blattner:b2937
Gene OrientationCounterclockwise
Centisome Percentage:66.40
Left Sequence End3080899
Right Sequence End3081819
Gene Sequence:
>921 bp
ATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTG
AATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTC
GACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATC
GCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGC
TTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGC
AAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCA
GAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCG
GTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGAA
AATGCTCTACAAGATGCATTAAGATTGGCATTTCAGCACGATGAAGAAGTATTGATTGAA
AAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCA
ATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAG
ACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGAGGCCAATTTGCAGGCATTA
GTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTG
GACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCAC
AGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGA
ATTCTGGAACTGGCGGACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:306
Protein Molecular Weight:33557
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Agmatinase
MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIR
QVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRML
SFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEFDHGTMFYTAPKEGLIDPNH
SVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMPVYLTFDIDCLDPAFAP
GTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQ
AAKKGE
References
External Links:
ResourceLink
Uniprot ID:P60651
Uniprot Name:SPEB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321973
Ecogene ID:EG10960
Ecocyc:EG10960
ColiBase:b2937
Kegg Gene:b2937
EchoBASE ID:EB0953
CCDB:SPEB_ECOLI
BacMap:16130838
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carvajal, N., Lopez, V., Salas, M., Uribe, E., Herrera, P., Cerpa, J. (1999). "Manganese is essential for catalytic activity of Escherichia coli agmatinase." Biochem Biophys Res Commun 258:808-811. Pubmed: 10329468
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Moore, R. C., Boyle, S. M. (1990). "Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli." J Bacteriol 172:4631-4640. Pubmed: 2198270
  • Szumanski, M. B., Boyle, S. M. (1990). "Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli." J Bacteriol 172:538-547. Pubmed: 2153656
  • Szumanski, M. B., Boyle, S. M. (1992). "Influence of cyclic AMP, agmatine, and a novel protein encoded by a flanking gene on speB (agmatine ureohydrolase) in Escherichia coli." J Bacteriol 174:758-764. Pubmed: 1310091