S-formylglutathione hydrolase frmB (P51025)

Identification
Name:S-formylglutathione hydrolase frmB
Synonyms:
  • FGH
Gene Name:frmB
Enzyme Class:
Biological Properties
General Function:Involved in carboxylesterase activity
Specific Function:Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate. Shows also esterase activity against two pNP-esters (pNP- acetate and pNP-propionate), alpha-naphthyl acetate and lactoylglutathione
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
  • Methane metabolism ec00680
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water ↔ 1.0Formic acid + 1.0Glutathione + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water ↔ 1.0Formic acid + 1.0Glutathione
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water ↔ 1.0Formic acid + 1.0Glutathione + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water → 1.0Hydrogen ion + 1.0Formic acid + 1.0Glutathione
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0S-Formylglutathione + 1.0Water → 1.0Glutathione + 1.0Formic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00142Formic acidMetaboCard
ECMDB00125GlutathioneMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01550S-FormylglutathioneMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cytoplasmic membrane-bounded vesicle
cytoplasmic vesicle
organelle
vesicle
Function
carboxylesterase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
S-formylglutathione hydrolase activity
thiolester hydrolase activity
Gene Properties
Blattner:b0355
Gene OrientationCounterclockwise
Centisome Percentage:8.12
Left Sequence End376759
Right Sequence End377592
Gene Sequence:
>834 bp
ATGGAACTCATTGAAAAACATGTCAGCTTTGGCGGCTGGCAAAATATGTATCGGCATTAT
TCCCAATCACTGAAATGTGAAATGAATGTCGGCGTCTATCTCCCACCAAAAGCCGCGAAT
GAAAAATTGCCGGTGCTGTACTGGCTTTCAGGCCTGACCTGCAACGAGCAGAATTTCATT
ACTAAATCGGGGATGCAGCGTTACGCGGCTGAGCACAACATTATTGTTGTTGCGCCGGAC
ACCAGTCCGCGAGGCAGTCATGTCGCAGATGCTGACCGTTACGATCTCGGGCAAGGTGCC
GGGTTTTACCTGAACGCGACGCAAGCGCCGTGGAATGAACATTACAAAATGTATGACTAT
ATCCGCAACGAGCTGCCGGATTTAGTGATGCATCATTTTCCGGCAACGGCCAAAAAGTCT
ATCTCTGGTCATTCTATGGGCGGGCTGGGCGCGCTGGTGCTGGCGTTACGTAACCCAGAT
GAATATGTCAGCGTCTCGGCGTTTTCGCCCATTGTCTCCCCATCGCAAGTGCCGTGGGGA
CAGCAAGCCTTTGCTGCATATCTTGCTGAAAATAAAGATGCCTGGTTGGATTACGACCCG
GTGAGTCTTATTTCACAAGGTCAACGCGTTGCGGAAATCATGGTTGATCAGGGGTTGAGT
GATGATTTTTACGCAGAACAGCTGCGGACTCCAAATCTTGAAAAGATCTGCCAGGAGATG
AATATCAAGACGTTAATCCGTTATCACGAGGGTTATGATCACAGCTATTATTTTGTCTCC
AGTTTTATTGGCGAGCATATTGCCTACCACGCCAATAAACTGAATATGCGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:277
Protein Molecular Weight:31424
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>S-formylglutathione hydrolase frmB
MELIEKHVSFGGWQNMYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFI
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
IRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQVPWG
QQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
References
External Links:
ResourceLink
Uniprot ID:P51025
Uniprot Name:SFGH1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674497
Ecogene ID:EG13295
Ecocyc:EG13295
ColiBase:b0355
Kegg Gene:b0355
EchoBASE ID:EB3080
CCDB:SFGH1_ECOLI
BacMap:16128340
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gonzalez, C. F., Proudfoot, M., Brown, G., Korniyenko, Y., Mori, H., Savchenko, A. V., Yakunin, A. F. (2006). "Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG." J Biol Chem 281:14514-14522. Pubmed: 16567800
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Herring, C. D., Blattner, F. R. (2004). "Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR." J Bacteriol 186:6714-6720. Pubmed: 15466022