ECMDB

Identification

Name:

Thermoresistant gluconokinase

Synonyms:

Gluconate kinase 2

Gene Name:

gntK

Enzyme Class:

2.7.1.12

Biological Properties

General Function:

Involved in shikimate kinase activity

Specific Function:

ATP + D-gluconate = ADP + 6-phospho-D- gluconate

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Pentose phosphate pathway ec00030

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.06-Phosphogluconic acid + 1.0ADP + 1.0Hydrogen ion
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.0ADP + 1.06-Phosphogluconic acid
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.06-Phosphogluconic acid + 1.0ADP + 1.0Hydrogen ion
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid ↔ 1.0ADP + 1.06-Phosphogluconic acid
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Gluconic acid → 1.0ADP + 1.06-Phosphogluconic acid
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01316	6-Phosphogluconic acid	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB00625	Gluconic acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
nucleoside binding
purine nucleoside binding
shikimate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
carbohydrate metabolic process
metabolic process
primary metabolic process

Gene Properties

Blattner:

b3437

Gene Orientation

Counterclockwise

Centisome Percentage:

77.05

Left Sequence End

3575088

Right Sequence End

3575615

Gene Sequence:

>528 bp
TTGAGCACGACTAACCATGATCACCACATTTACGTCTTGATGGGCGTATCGGGCAGCGGC
AAATCTGCGGTCGCCAGTGAAGTGGCGCATCAACTTCATGCCGCGTTTCTTGATGGCGAT
TTCCTCCATCCACGGCGCAATATCGAAAAAATGGCGTCTGGCGAACCACTGAATGACGAC
GATCGCAAACCGTGGTTGCAGGCGCTGAACGACGCCGCGTTTGCTATGCAGCGCACTAAT
AAAGTGTCGCTGATCGTCTGTTCTGCATTGAAAAAACACTATCGCGACTTGCTGCGTGAA
GGTAATCCGAATCTCTCTTTCATCTATTTGAAAGGCGATTTTGATGTGATTGAAAGCCGC
CTGAAAGCGCGCAAAGGCCATTTCTTTAAAACCCAAATGTTGGTGACGCAGTTTGAAACG
CTGCAGGAGCCGGGTGCGGACGAAACCGATGTACTGGTGGTGGATATCGATCAACCGCTG
GAAGGTGTTGTGGCAAGCACCATTGAGGTTATTAAAAAAGGCAAATAA

Protein Properties

Pfam Domain Function:

SKI (PF01202 )

Protein Residues:

175

Protein Molecular Weight:

19543

Protein Theoretical pI:

PDB File:

1KOF

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Thermoresistant gluconokinase
MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHPRRNIEKMASGEPLNDD
DRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGNPNLSFIYLKGDFDVIESR
LKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPLEGVVASTIEVIKKGK

References

External Links:

Resource	Link
Uniprot ID:	P46859
Uniprot Name:	GNTK_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85676606
PDB ID:	1KOF
Ecogene ID:	EG12629
Ecocyc:	EG12629
ColiBase:	b3437
Kegg Gene:	b3437
EchoBASE ID:	EB2513
CCDB:	GNTK_ECOLI
BacMap:	90111591

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Izu, H., Adachi, O., Yamada, M. (1997). "Gene organization and transcriptional regulation of the gntRKU operon involved in gluconate uptake and catabolism of Escherichia coli." J Mol Biol 267:778-793. Pubmed: 9135111
Kraft, L., Sprenger, G. A., Lindqvist, Y. (2001). "Crystallization and preliminary X-ray crystallographic studies of recombinant thermoresistant gluconate kinase GntK from Escherichia coli." Acta Crystallogr D Biol Crystallogr 57:1159-1161. Pubmed: 11468405
Tong, S., Porco, A., Isturiz, T., Conway, T. (1996). "Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism." J Bacteriol 178:3260-3269. Pubmed: 8655507

Thermoresistant gluconokinase (P46859)